granted patent: Methods for enhancing the production of viral vaccines in PKR-deficient cell culture

TitleMethods for enhancing the production of viral vaccines in PKR-deficient cell culture
Granted PatentUS 5840565
Granted Date1998-11-24
Priority Date1996-08-21 US 08/700198
1995-08-22 US 08/002621P
Inventors
Issue Date1998
Citation
US Patent US 5840565. Washington, DC: US Patent and Trademark Office (USPTO), 1998 How to Cite?
AbstractMethods for enhancing the production of viral vaccines in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain interferon induced antiviral activities, in particular, cellular levels of double-stranded RNA (dsRNA) dependent kinase (PKR), PKR-deficient cells are obtained by a) transfecting a parent cell line with a PKR antisense polynucleotide; b) unaided uptake into a cell line by culturing said cell line in the presence of a PKR antisense polynucleotide; c) transfection of a parent cell line with a PKR dominant negative mutant; or d) culturing a cell line in the presence of 2-aminopurine. In cell cultures deficient for PKR, viral yield is enhanced by several orders of magnitude over cell cultures with normal levels of these proteins making these cell cultures useful for the production of viral vaccines.
Persistent Identifierhttp://hdl.handle.net/10722/177027

 

DC FieldValueLanguage
dc.date.accessioned2012-11-30T08:38:58Z-
dc.date.available2012-11-30T08:38:58Z-
dc.date.issued1998-
dc.identifier.citationUS Patent US 5840565. Washington, DC: US Patent and Trademark Office (USPTO), 1998en_HK
dc.identifier.urihttp://hdl.handle.net/10722/177027-
dc.description.abstractMethods for enhancing the production of viral vaccines in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain interferon induced antiviral activities, in particular, cellular levels of double-stranded RNA (dsRNA) dependent kinase (PKR), PKR-deficient cells are obtained by a) transfecting a parent cell line with a PKR antisense polynucleotide; b) unaided uptake into a cell line by culturing said cell line in the presence of a PKR antisense polynucleotide; c) transfection of a parent cell line with a PKR dominant negative mutant; or d) culturing a cell line in the presence of 2-aminopurine. In cell cultures deficient for PKR, viral yield is enhanced by several orders of magnitude over cell cultures with normal levels of these proteins making these cell cultures useful for the production of viral vaccines.en_HK
dc.relation.isreferencedbyUS 2009017523 (A1) 2009-01-15en_HK
dc.relation.isreferencedbyUS 8124106 (B2) 2012-02-28en_HK
dc.relation.isreferencedbyUS 2004157310 (A1) 2004-08-12en_HK
dc.relation.isreferencedbyUS 7132271 (B2) 2006-11-07en_HK
dc.relation.isreferencedbyUS 7183107 (B2) 2007-02-27en_HK
dc.relation.isreferencedbyUS 2004076612 (A1) 2004-04-22en_HK
dc.relation.isreferencedbyUS 6762038 (B1) 2004-07-13en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License For Public Patent Documentsen_US
dc.titleMethods for enhancing the production of viral vaccines in PKR-deficient cell cultureen_HK
dc.typePatenten_US
dc.description.naturepublished_or_final_versionen_US
dc.contributor.inventorLau, ASYen_HK
patents.identifier.applicationUS 08/700198en_HK
patents.identifier.grantedUS 5840565en_HK
patents.description.assigneeUNIV CALIFORNIA [US]en_HK
patents.description.countryUnited States of Americaen_HK
patents.date.granted1998-11-24en_HK
patents.date.application1996-08-21en_HK
patents.date.priority1996-08-21 US 08/700198en_HK
patents.date.priority1995-08-22 US 08/002621Pen_HK
patents.description.ccUSen_HK
patents.relation.familyAT 289348 (T) 2005-03-15en_HK
patents.relation.familyAU 6860896 (A) 1997-03-19en_HK
patents.relation.familyAU 707130 (B2) 1999-07-01en_HK
patents.relation.familyAU 6857696 (A) 1997-03-19en_HK
patents.relation.familyBR 9610550 (A) 1999-07-06en_HK
patents.relation.familyCA 2229163 (A1) 1997-03-06en_HK
patents.relation.familyCA 2229405 (A1) 1997-03-06en_HK
patents.relation.familyCN 1200148 (A) 1998-11-25en_HK
patents.relation.familyCN 1161469 (C) 2004-08-11en_HK
patents.relation.familyDE 69634360 (T2) 2005-12-29en_HK
patents.relation.familyEP 0846160 (A1) 1998-06-10en_HK
patents.relation.familyEP 0846174 (A1) 1998-06-10en_HK
patents.relation.familyJP H11511324 (A) 1999-10-05en_HK
patents.relation.familyJP 3432519 (B2) 2003-08-04en_HK
patents.relation.familyJP H11514344 (A) 1999-12-07en_HK
patents.relation.familyJP 4267693 (B2) 2009-05-27en_HK
patents.relation.familyMX 9801423 (A) 1998-05-31en_HK
patents.relation.familyUS 5840565 (A) 1998-11-24en_HK
patents.relation.familyUS 6159712 (A) 2000-12-12en_HK
patents.relation.familyUS 6489144 (B1) 2002-12-03en_HK
patents.relation.familyUS 2001001290 (A1) 2001-05-17en_HK
patents.relation.familyUS 6673591 (B2) 2004-01-06en_HK
patents.relation.familyUS 2001001709 (A1) 2001-05-24en_HK
patents.relation.familyUS 6686190 (B2) 2004-02-03en_HK
patents.relation.familyUS 2001055791 (A1) 2001-12-27en_HK
patents.relation.familyUS 6855519 (B2) 2005-02-15en_HK
patents.relation.familyUS 2004157310 (A1) 2004-08-12en_HK
patents.relation.familyUS 7132271 (B2) 2006-11-07en_HK
patents.relation.familyUS 2004219642 (A1) 2004-11-04en_HK
patents.relation.familyWO 9708292 (A1) 1997-03-06en_HK
patents.relation.familyWO 9708324 (A1) 1997-03-06en_HK
patents.description.kindAen_HK
patents.typePatent_granteden_HK

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