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Article: Epigenetic markers for noninvasive early detection of nasopharyngeal carcinoma by methylation-sensitive high resolution melting

TitleEpigenetic markers for noninvasive early detection of nasopharyngeal carcinoma by methylation-sensitive high resolution melting
Authors
KeywordsBiomarker
DNA methylation
NPC
MS‐HRM
Early diagnosis
Issue Date2015
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal of Cancer, 2015, v. 136 n. 4, p. E127-E135 How to Cite?
AbstractNasopharyngeal carcinoma (NPC) is a human malignancy that is closely associated with Epstein‐Barr Virus (EBV). Early diagnosis of NPC will greatly improve the overall survival. However, current EBV DNA marker detection still lacks the predictive value to perform well in high‐risk populations for early detection of NPC. Since aberrant promoter hypermethylation of tumor suppressor genes (TSGs) is widely considered to be an important epigenetic change in early carcinogenesis, this study identified a panel of methylation markers for early detection of NPC and also assessed the clinical usefulness of these markers with noninvasive plasma specimens instead of biopsies. MS‐HRM assays were carried out to assess the methylation status of a selected panel of four TSGs (RASSF1A, WIF1, DAPK1 and RARβ2) in biopsies, NP brushings and cell‐free plasma from NPC patients. High‐risk and cancer‐free groups were used as controls. DNA methylation panel showed higher sensitivity and specificity than EBV DNA marker in cell‐free plasma from NPC patients at early Stages (I and II) and in addition to the EBV DNA marker, MS‐HRM test for plasma and NP brushing DNA methylation significantly increased the detection rate at all NPC stages as well as local recurrence, using this selected four‐gene panel (p < 0.05). MS‐HRM assay on a selected gene panel has great potential to become a noninvasive and complementary test for NPC early and recurrent detection in combination with the EBV DNA test to increase the sensitivity for NPC detection at an early stage.
Persistent Identifierhttp://hdl.handle.net/10722/202757
ISSN
2019 Impact Factor: 5.145
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, X-
dc.contributor.authorDai, W-
dc.contributor.authorKwong, DLW-
dc.contributor.authorSzeto, CYY-
dc.contributor.authorWong, EHW-
dc.contributor.authorNg, WT-
dc.contributor.authorLee, AWM-
dc.contributor.authorNgan, KC-
dc.contributor.authorYau, CC-
dc.contributor.authorTung, SY-
dc.contributor.authorLung, ML-
dc.date.accessioned2014-09-19T09:33:36Z-
dc.date.available2014-09-19T09:33:36Z-
dc.date.issued2015-
dc.identifier.citationInternational Journal of Cancer, 2015, v. 136 n. 4, p. E127-E135-
dc.identifier.issn0020-7136-
dc.identifier.urihttp://hdl.handle.net/10722/202757-
dc.description.abstractNasopharyngeal carcinoma (NPC) is a human malignancy that is closely associated with Epstein‐Barr Virus (EBV). Early diagnosis of NPC will greatly improve the overall survival. However, current EBV DNA marker detection still lacks the predictive value to perform well in high‐risk populations for early detection of NPC. Since aberrant promoter hypermethylation of tumor suppressor genes (TSGs) is widely considered to be an important epigenetic change in early carcinogenesis, this study identified a panel of methylation markers for early detection of NPC and also assessed the clinical usefulness of these markers with noninvasive plasma specimens instead of biopsies. MS‐HRM assays were carried out to assess the methylation status of a selected panel of four TSGs (RASSF1A, WIF1, DAPK1 and RARβ2) in biopsies, NP brushings and cell‐free plasma from NPC patients. High‐risk and cancer‐free groups were used as controls. DNA methylation panel showed higher sensitivity and specificity than EBV DNA marker in cell‐free plasma from NPC patients at early Stages (I and II) and in addition to the EBV DNA marker, MS‐HRM test for plasma and NP brushing DNA methylation significantly increased the detection rate at all NPC stages as well as local recurrence, using this selected four‐gene panel (p < 0.05). MS‐HRM assay on a selected gene panel has great potential to become a noninvasive and complementary test for NPC early and recurrent detection in combination with the EBV DNA test to increase the sensitivity for NPC detection at an early stage.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home-
dc.relation.ispartofInternational Journal of Cancer-
dc.subjectBiomarker-
dc.subjectDNA methylation-
dc.subjectNPC-
dc.subjectMS‐HRM-
dc.subjectEarly diagnosis-
dc.titleEpigenetic markers for noninvasive early detection of nasopharyngeal carcinoma by methylation-sensitive high resolution melting-
dc.typeArticle-
dc.identifier.emailDai, W: weidai2@hku.hk-
dc.identifier.emailKwong, DLW: dlwkwong@hku.hk-
dc.identifier.emailSzeto, CYY: cyyszeto@hku.hk-
dc.identifier.emailWong, EHW: elibe@hku.hk-
dc.identifier.emailNg, WT: ngwt1@hkucc.hku.hk-
dc.identifier.emailLee, AWM: awmlee@hkucc.hku.hk-
dc.identifier.emailNgan, KC: rkcngan@hkucc.hku.hk-
dc.identifier.emailYau, CC: yaucc@hkucc.hku.hk-
dc.identifier.emailLung, ML: mlilung@hku.hk-
dc.identifier.authorityDai, W=rp02146-
dc.identifier.authorityKwong, DLW=rp00414-
dc.identifier.authorityNg, WT=rp02671-
dc.identifier.authorityLee, AWM=rp02056-
dc.identifier.authorityNgan, KC=rp02371-
dc.identifier.authorityLung, ML=rp00300-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/ijc.29192-
dc.identifier.pmid25196065-
dc.identifier.scopuseid_2-s2.0-84918526343-
dc.identifier.hkuros240234-
dc.identifier.volume136-
dc.identifier.issue4-
dc.identifier.spageE127-
dc.identifier.epageE135-
dc.identifier.isiWOS:000346089900012-
dc.publisher.placeUnited States-

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