Conference Paper: Polymorphisms of the ACE2 and CD13 (ANPEP) genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong

TitlePolymorphisms of the ACE2 and CD13 (ANPEP) genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong
Authors
Issue Date2004
Citation
HUGO HGM2004 - Human Genome Meeting, Berlin, Germay, 4-7 April 2004, In Poster Abstracts of HUGO HGM2004, p. Poster 159 How to Cite?
AbstractAngiotensin-converting enzyme 2 (ACE2), mapped on Xp22, has been recently found to be a receptor for the coronavirus causing severe acute respiratory syndrome (SARS-CoV). CD13 (ANPEP), alanyl (membrane) aminopeptidase mapped on 15q25-q26, is also a receptor for a number of coronaviruses. We investigated whether genetic polymorphisms of ACE2 and ANPEP might be associated with the susceptibility SARS-CoV infection in Chinese in Hong Kong. DNA extracted from 215 (female:127, male:88) SARS-CoV patients confirmed by either serology or RT-PCR assay, 177 (female:142, male:35) unaffected health care workers (HCW), and 264 (female:174, male:90) unaffected normal controls (NC) were genotyped for single nucleotide polymorphisms (SNPs) of ACE2 and ANPEP by the high-throughput Sequenom system. 4/12 and 8/12 SNPs of ACE2 and ANPEP respectively were found to have allele frequency >5% in 90 NC samples. These SNPs were further genotyped in the three populations. All the studied SNPs are in Hardy-Weinberg equilibrium in the three populations. As ACE2 is located on sex chromosome, statistical analysis was performed on female and male subgroups. Except for ACE2 SNP rs879922 in female, no significant association was found for the other SNPs tested, both in male and female (p>0.05). The C-allele carriers of SNP rs879922 (dbSNP cluster id) appeared to be associated with lower risk to develop SARS (p=0.004); however, the number of C-allele carriers is too small (6.4% in female, and 2.5% in male in the overall populations) to draw proper conclusions. The G-allele carriers of ANPEP SNP A603G appeared associated with risk (p=0.008). However, allowing for multiple testing, this p value does not yet reach statistical significance. No significant association was found for all the other ANPEP SNPs tested. The presence of other SNPs in the promoters or coding regions of ACE2 and ANPEP are being explored by direct sequencing on the pooled DNA with view to genotyping.
Persistent Identifierhttp://hdl.handle.net/10722/96106

 

DC FieldValueLanguage
dc.contributor.authorChan, YKen_HK
dc.contributor.authorSong, Yen_HK
dc.contributor.authorMak, WKen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorYip, SPen_HK
dc.contributor.authorTin, PCen_HK
dc.contributor.authorLiu, Wen_HK
dc.contributor.authorXue, Wen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorKhoo, USen_HK
dc.date.accessioned2010-09-25T16:23:34Z-
dc.date.available2010-09-25T16:23:34Z-
dc.date.issued2004en_HK
dc.identifier.citationHUGO HGM2004 - Human Genome Meeting, Berlin, Germay, 4-7 April 2004, In Poster Abstracts of HUGO HGM2004, p. Poster 159-
dc.identifier.urihttp://hdl.handle.net/10722/96106-
dc.description.abstractAngiotensin-converting enzyme 2 (ACE2), mapped on Xp22, has been recently found to be a receptor for the coronavirus causing severe acute respiratory syndrome (SARS-CoV). CD13 (ANPEP), alanyl (membrane) aminopeptidase mapped on 15q25-q26, is also a receptor for a number of coronaviruses. We investigated whether genetic polymorphisms of ACE2 and ANPEP might be associated with the susceptibility SARS-CoV infection in Chinese in Hong Kong. DNA extracted from 215 (female:127, male:88) SARS-CoV patients confirmed by either serology or RT-PCR assay, 177 (female:142, male:35) unaffected health care workers (HCW), and 264 (female:174, male:90) unaffected normal controls (NC) were genotyped for single nucleotide polymorphisms (SNPs) of ACE2 and ANPEP by the high-throughput Sequenom system. 4/12 and 8/12 SNPs of ACE2 and ANPEP respectively were found to have allele frequency >5% in 90 NC samples. These SNPs were further genotyped in the three populations. All the studied SNPs are in Hardy-Weinberg equilibrium in the three populations. As ACE2 is located on sex chromosome, statistical analysis was performed on female and male subgroups. Except for ACE2 SNP rs879922 in female, no significant association was found for the other SNPs tested, both in male and female (p>0.05). The C-allele carriers of SNP rs879922 (dbSNP cluster id) appeared to be associated with lower risk to develop SARS (p=0.004); however, the number of C-allele carriers is too small (6.4% in female, and 2.5% in male in the overall populations) to draw proper conclusions. The G-allele carriers of ANPEP SNP A603G appeared associated with risk (p=0.008). However, allowing for multiple testing, this p value does not yet reach statistical significance. No significant association was found for all the other ANPEP SNPs tested. The presence of other SNPs in the promoters or coding regions of ACE2 and ANPEP are being explored by direct sequencing on the pooled DNA with view to genotyping.-
dc.languageengen_HK
dc.relation.ispartofPoster Abstracts of HUGO HGM2004en_HK
dc.titlePolymorphisms of the ACE2 and CD13 (ANPEP) genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kongen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChan, YK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailSong, Y: songy@hkucc.hku.hken_HK
dc.identifier.emailMak, WK: wkmaka@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailTin, PC: pctin@hkucc.hku.hken_HK
dc.identifier.emailChan, LC: chanlc@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailKhoo, US: uskhoo@pathology.hku.hken_HK
dc.identifier.authorityChan, YK=rp00453en_HK
dc.identifier.authoritySong, Y=rp00488en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.hkuros97804en_HK
dc.identifier.hkuros88192-
dc.identifier.hkuros87736-
dc.identifier.spagePoster 159-
dc.identifier.epagePoster 159-
dc.description.otherHUGO HGM2004 - Human Genome Meeting, Berlin, Germay, 4-7 April 2004, In Poster Abstracts of HUGO HGM2004, p. Poster 159-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats