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Patent History
- ApplicationUS 11/892579 2004-07-16
- PublicationUS 20050084970 2005-04-21
- GrantedUS 7781190 2010-08-24
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granted patent: Method For Constructing And Modifying Large Dna Molecules
Title | Method For Constructing And Modifying Large Dna Molecules |
---|---|
Granted Patent | US 7781190 |
Granted Date | 2010-08-24 |
Priority Date | 2004-07-16 US 11/892579 2003-07-24 US 10/490025P |
Inventors | |
Issue Date | 2010 |
Citation | US Patent 7781190. Washington, DC: US Patent and Trademark Office (USPTO), 2010 How to Cite? |
Abstract | This Invention Provides A Method For Combining Overlapping Dna Molecules Comprising: (A) Providing First And Second Dna Fragments, The First Having A Region Homologous To A Region In The Second; (B) Tagging The First Dna Fragment With A Selectable Marker; (C) Cloning The First Dna Sequence Into A Retrieval Vector To Form A Dna-Vector Complex; (D) Linearizing The Dna-Vector Complex; And (E) Inserting The First Dna Fragment From The Dna-Vector Complex Into The Second Dna Fragment Using Homologous Recombination To Form A Combined Dna Molecule; And (F) Removing The Selectable Marker, Thereby Generating A Combined Dna Molecule. The Invention Further Provides A Vector For Retrieving And Inserting A Selected Dna Molecule Into A Target Dna Molecule. |
Persistent Identifier | http://hdl.handle.net/10722/142070 |
References |
DC Field | Value | Language |
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dc.date.accessioned | 2011-10-19T06:29:09Z | - |
dc.date.available | 2011-10-19T06:29:09Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | US Patent 7781190. Washington, DC: US Patent and Trademark Office (USPTO), 2010 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/142070 | - |
dc.description.abstract | This Invention Provides A Method For Combining Overlapping Dna Molecules Comprising: (A) Providing First And Second Dna Fragments, The First Having A Region Homologous To A Region In The Second; (B) Tagging The First Dna Fragment With A Selectable Marker; (C) Cloning The First Dna Sequence Into A Retrieval Vector To Form A Dna-Vector Complex; (D) Linearizing The Dna-Vector Complex; And (E) Inserting The First Dna Fragment From The Dna-Vector Complex Into The Second Dna Fragment Using Homologous Recombination To Form A Combined Dna Molecule; And (F) Removing The Selectable Marker, Thereby Generating A Combined Dna Molecule. The Invention Further Provides A Vector For Retrieving And Inserting A Selected Dna Molecule Into A Target Dna Molecule. | en_HK |
dc.relation.isreferencedby | US 2005084970 (A1) 2005-04-21 | en_HK |
dc.relation.isreferencedby | US 7781190 (B2) 2010-08-24 | en_HK |
dc.title | Method For Constructing And Modifying Large Dna Molecules | en_HK |
dc.type | Patent | en_US |
dc.identifier.email | Huang, Jiandong:jdhuang@hku.hk | en_US |
dc.identifier.email | Tanner, Julian Alexander:jatanner@hkucc.hku.hk | en_US |
dc.identifier.email | Zhang, Xin Mei: | en_US |
dc.description.nature | published_or_final_version | - |
dc.identifier.hkuros | 100401 | en_US |
dc.contributor.inventor | Huang, Jiandong | en_US |
dc.contributor.inventor | Tanner, Julian Alexander | en_US |
dc.contributor.inventor | Zhang, Xin Mei | en_US |
patents.identifier.application | US 11/892579 | en_HK |
patents.identifier.granted | US 7781190 | en_HK |
patents.description.assignee | Univ Hong Kong [Cn] | en_HK |
patents.description.country | United States of America | en_HK |
patents.date.publication | 2005-04-21 | en_HK |
patents.date.granted | 2010-08-24 | en_HK |
dc.relation.references | US 6355412 (B1) 2002-03-12 | en_HK |
dc.relation.references | US 2005084970 (A1) 2005-04-21 | en_HK |
dc.relation.references | US 7781190 (B2) 2010-08-24 | en_HK |
patents.identifier.hkutechid | Bioch-2003-00136-1 | en_US |
patents.date.application | 2004-07-16 | en_HK |
patents.date.priority | 2004-07-16 US 11/892579 | en_HK |
patents.date.priority | 2003-07-24 US 10/490025P | en_HK |
patents.description.cc | US | en_HK |
patents.identifier.publication | US 20050084970 | en_HK |
patents.relation.family | US 2005084970 (A1) 2005-04-21 | en_HK |
patents.relation.family | US 7781190 (B2) 2010-08-24 | en_HK |
patents.relation.family | WO 2005010179 (A1) 2005-02-03 | en_HK |
patents.description.kind | B2 | en_HK |
patents.type | Patent_granted | en_HK |