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  Patent History
  • Application
    US 10/892579 2004-07-16
  • Publication
    US 2005084970 2005-04-21
Supplementary

published patent: Method for constructing and modifying large DNA molecules

TitleMethod for constructing and modifying large DNA molecules
Priority Date2004-07-16 US 10/892579
2003-07-24 US 10/490025P
Inventors
Issue Date2005
Citation
US Published patent application US 2005084970. Washington, DC: US Patent and Trademark Office (USPTO), 2005 How to Cite?
AbstractThis invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.
Persistent Identifierhttp://hdl.handle.net/10722/176892

 

DC FieldValueLanguage
dc.date.accessioned2012-11-30T08:38:41Z-
dc.date.available2012-11-30T08:38:41Z-
dc.date.issued2005-
dc.identifier.citationUS Published patent application US 2005084970. Washington, DC: US Patent and Trademark Office (USPTO), 2005en_HK
dc.identifier.urihttp://hdl.handle.net/10722/176892-
dc.description.abstractThis invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.en_HK
dc.relation.isreferencedbyUS 7781190 (B2) 2010-08-24en_HK
dc.relation.isreferencedbyUS 2005084970 (A1) 2005-04-21en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License For Public Patent Documentsen_US
dc.titleMethod for constructing and modifying large DNA moleculesen_HK
dc.typePatenten_US
dc.description.naturepublished_or_final_versionen_US
dc.contributor.inventorHuang, Jen_HK
dc.contributor.inventorZhang Xin-Meien_HK
dc.contributor.inventorTanner, JAen_HK
patents.identifier.applicationUS 10/892579en_HK
patents.description.assigneeHUANG JIAN-DONG, ; ZHANG XIN-MEI, ; TANNER JULIAN A, ; THE UNIVERSITY OF HONG KONGen_HK
patents.description.countryUnited States of Americaen_HK
patents.date.publication2005-04-21en_HK
patents.date.application2004-07-16en_HK
patents.date.priority2004-07-16 US 10/892579en_HK
patents.date.priority2003-07-24 US 10/490025Pen_HK
patents.description.ccUSen_HK
patents.identifier.publicationUS 2005084970en_HK
patents.relation.familyUS 2005084970 (A1) 2005-04-21en_HK
patents.relation.familyUS 7781190 (B2) 2010-08-24en_HK
patents.relation.familyWO 2005010179 (A1) 2005-02-03en_HK
patents.description.kindA1en_HK
patents.typePatent_publisheden_HK

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