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  Patent History
  • Application
    PC T/CN2004000858 2004-07-23
  • Publication
    WO 2005010179 2005-02-03
Supplementary

published patent: METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES

TitleMETHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES
Priority Date2003-07-24 US 10/490025P
Inventors
Issue Date2005
Citation
WO Published patent application WO 2005010179. World Intellectual Property Organization (WIPO), PatentScope, 2005 How to Cite?
AbstractThis invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.
Persistent Identifierhttp://hdl.handle.net/10722/177093
References

 

DC FieldValueLanguage
dc.date.accessioned2012-11-30T08:39:04Z-
dc.date.available2012-11-30T08:39:04Z-
dc.date.issued2005-
dc.identifier.citationWO Published patent application WO 2005010179. World Intellectual Property Organization (WIPO), PatentScope, 2005en_HK
dc.identifier.urihttp://hdl.handle.net/10722/177093-
dc.description.abstractThis invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License For Public Patent Documentsen_US
dc.titleMETHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULESen_HK
dc.typePatenten_US
dc.description.naturepublished_or_final_versionen_US
dc.contributor.inventorHuang, Jen_HK
dc.contributor.inventorTanner, JAen_HK
dc.contributor.inventorZhang, Xinmeien_HK
patents.identifier.applicationPC T/CN2004000858en_HK
patents.description.assigneeUNIV HONG KONG [CN]en_HK
patents.description.countryWorld Intellectual Property Organization (WIPO)en_HK
patents.date.publication2005-02-03en_HK
dc.relation.referencesWO 9808964 (A1) 1998-03-05en_HK
dc.relation.referencesWO 0026396 (A1) 2000-05-11en_HK
patents.date.application2004-07-23en_HK
patents.date.priority2003-07-24 US 10/490025Pen_HK
patents.description.ccWOen_HK
patents.identifier.publicationWO 2005010179en_HK
patents.relation.familyUS 2005084970 (A1) 2005-04-21en_HK
patents.relation.familyUS 7781190 (B2) 2010-08-24en_HK
patents.relation.familyWO 2005010179 (A1) 2005-02-03en_HK
patents.description.kindA1en_HK
patents.typePatent_publisheden_HK

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