Quantitative assessment of magnetically labeled luciferase positive cells using multimodality imaging

Abstract

Cellular imaging using magnetically labeled luciferase positive cells with multimodality imaging has found numerous biological applications. However, studies only involve imaging dual-labeled cells, tracking their migrations without quantification of local density of the dual-labeled cells. Quantitative analysis of cells after transplantation may allow more accurate assessment of cell delivery and subsequent distribution and migration, which can lead to more effective monitoring and optimization of therapeutic paradigms. In this study, we demonstrated that dual-labeled cells exhibited excellent linear correlations between cell concentration with photon flux and δR2* in vitro, illustrating that quantitative assessment of dual-labeled cells can be achieved.