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- PMID: 11815675
- WOS: WOS:000173436500006
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Article: Cigarette smoke extracts delay wound healing in the stomach: Involvement of polyamine synthesis
Title | Cigarette smoke extracts delay wound healing in the stomach: Involvement of polyamine synthesis |
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Authors | |
Keywords | Cigarette smoke extracts Gastric ulcer healing Myeloperoxidase Ornithine decarboxylase |
Issue Date | 2002 |
Publisher | Society for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/ |
Citation | Experimental Biology And Medicine, 2002, v. 227 n. 2, p. 114-124 How to Cite? |
Abstract | The association between cigarette smoking and peptic ulcer diseases has been well established. Ornithine decarboxylase (ODC) is crucial for the gastroprotective and mucosal growth promoting effects in gastric ulcer healing. The aim of this study is to elucidate the possible mechanism of how inhibition of ODC activity is involved in the delay of ulcer healing, if any, by cigarette smoke extracts (CSE). CSE were fractionated into chloroform extract (CE) and ethanol extract (EE). In in vivo study, rats with acetic acid-induced ulcers were given CE or EE intragastrically (2.5 or 5 mg/kg) once daily for 3 days. Ulcer sizes were significantly larger after CE or EE administration, followed by an increase in myeloperoxidase activity and a reduction in cell proliferation. However, both CSE stimulated the number of microvessels following the increase of basic fibroblast growth factor. In in vitro studies, the effect of CE or EE (10, 40, or 100 μg/ml) on cell migration and cell proliferation were measured using an in vitro wound model and [3H]-thymidine incorporation assay, respectively. Both CSE delayed cell migration and decreased cell proliferation, which were accompanied with a reduction in ODC activity. Exogenous spermidine (5 or 10 μM) could reverse the inhibitory action on cell proliferation and ODC activity induced by CSE. In conclusion, both CSE significantly delayed ulcer healing as a result of reduction in cell proliferation and cell migration. All these effects are, in part, related to the reduction of polyamine synthesis. |
Persistent Identifier | http://hdl.handle.net/10722/80268 |
ISSN | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Shin, VY | en_HK |
dc.contributor.author | Liu, ESL | en_HK |
dc.contributor.author | Koo, MWL | en_HK |
dc.contributor.author | Wang, JY | en_HK |
dc.contributor.author | Matsui, H | en_HK |
dc.contributor.author | Cho, CH | en_HK |
dc.date.accessioned | 2010-09-06T08:04:25Z | - |
dc.date.available | 2010-09-06T08:04:25Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | Experimental Biology And Medicine, 2002, v. 227 n. 2, p. 114-124 | en_HK |
dc.identifier.issn | 0037-9727 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/80268 | - |
dc.description.abstract | The association between cigarette smoking and peptic ulcer diseases has been well established. Ornithine decarboxylase (ODC) is crucial for the gastroprotective and mucosal growth promoting effects in gastric ulcer healing. The aim of this study is to elucidate the possible mechanism of how inhibition of ODC activity is involved in the delay of ulcer healing, if any, by cigarette smoke extracts (CSE). CSE were fractionated into chloroform extract (CE) and ethanol extract (EE). In in vivo study, rats with acetic acid-induced ulcers were given CE or EE intragastrically (2.5 or 5 mg/kg) once daily for 3 days. Ulcer sizes were significantly larger after CE or EE administration, followed by an increase in myeloperoxidase activity and a reduction in cell proliferation. However, both CSE stimulated the number of microvessels following the increase of basic fibroblast growth factor. In in vitro studies, the effect of CE or EE (10, 40, or 100 μg/ml) on cell migration and cell proliferation were measured using an in vitro wound model and [3H]-thymidine incorporation assay, respectively. Both CSE delayed cell migration and decreased cell proliferation, which were accompanied with a reduction in ODC activity. Exogenous spermidine (5 or 10 μM) could reverse the inhibitory action on cell proliferation and ODC activity induced by CSE. In conclusion, both CSE significantly delayed ulcer healing as a result of reduction in cell proliferation and cell migration. All these effects are, in part, related to the reduction of polyamine synthesis. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Society for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/ | en_HK |
dc.relation.ispartof | Experimental Biology and Medicine | en_HK |
dc.subject | Cigarette smoke extracts | - |
dc.subject | Gastric ulcer healing | - |
dc.subject | Myeloperoxidase | - |
dc.subject | Ornithine decarboxylase | - |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Cell Division | en_HK |
dc.subject.mesh | Cell Movement | en_HK |
dc.subject.mesh | Gastric Mucosa - metabolism - pathology | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Polyamines - metabolism | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.subject.mesh | Smoking - adverse effects - metabolism - physiopathology | en_HK |
dc.subject.mesh | Stomach Ulcer - etiology - metabolism - physiopathology | en_HK |
dc.subject.mesh | Wound Healing | en_HK |
dc.title | Cigarette smoke extracts delay wound healing in the stomach: Involvement of polyamine synthesis | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1535-3702&volume=227 &issue=2&spage=114&epage=124&date=2002&atitle=Cigarette+smoke+extracts+delay+wound+healing+in+the+stomach:+Involvement+of+polyamine+synthesis | en_HK |
dc.identifier.email | Koo, MWL: wlkoo@hku.hk | en_HK |
dc.identifier.authority | Koo, MWL=rp00233 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.pmid | 11815675 | - |
dc.identifier.scopus | eid_2-s2.0-0036486325 | en_HK |
dc.identifier.hkuros | 76683 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036486325&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 227 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 114 | en_HK |
dc.identifier.epage | 124 | en_HK |
dc.identifier.isi | WOS:000173436500006 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Shin, VY=7003491170 | en_HK |
dc.identifier.scopusauthorid | Liu, ESL=7202240071 | en_HK |
dc.identifier.scopusauthorid | Koo, MWL=7004550899 | en_HK |
dc.identifier.scopusauthorid | Wang, JY=8438337300 | en_HK |
dc.identifier.scopusauthorid | Matsui, H=7401527136 | en_HK |
dc.identifier.scopusauthorid | Cho, CH=14067000400 | en_HK |
dc.identifier.issnl | 0037-9727 | - |