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- Publisher Website: 10.1016/j.jviromet.2007.01.015
- Scopus: eid_2-s2.0-34247163576
- PMID: 17324474
- WOS: WOS:000246675900029
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Article: Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes
Title | Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes |
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Authors | |
Keywords | Consensus primers Influenza virus Polymerase chain reaction Polymerase genes |
Issue Date | 2007 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet |
Citation | Journal Of Virological Methods, 2007, v. 142 n. 1-2, p. 218-222 How to Cite? |
Abstract | Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. © 2007 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/79301 |
ISSN | 2023 Impact Factor: 2.2 2023 SCImago Journal Rankings: 0.510 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, OTW | en_HK |
dc.contributor.author | Barr, I | en_HK |
dc.contributor.author | Leung, CYH | en_HK |
dc.contributor.author | Chen, H | en_HK |
dc.contributor.author | Guan, Y | en_HK |
dc.contributor.author | Peiris, JSM | en_HK |
dc.contributor.author | Poon, LLM | en_HK |
dc.date.accessioned | 2010-09-06T07:53:02Z | - |
dc.date.available | 2010-09-06T07:53:02Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | Journal Of Virological Methods, 2007, v. 142 n. 1-2, p. 218-222 | en_HK |
dc.identifier.issn | 0166-0934 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/79301 | - |
dc.description.abstract | Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. © 2007 Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet | en_HK |
dc.relation.ispartof | Journal of Virological Methods | en_HK |
dc.rights | Journal of Virological Methods. Copyright © Elsevier BV. | en_HK |
dc.subject | Consensus primers | en_HK |
dc.subject | Influenza virus | en_HK |
dc.subject | Polymerase chain reaction | en_HK |
dc.subject | Polymerase genes | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Base Sequence | en_HK |
dc.subject.mesh | Consensus Sequence | en_HK |
dc.subject.mesh | DNA Primers | en_HK |
dc.subject.mesh | Hemagglutinin Glycoproteins, Influenza Virus - classification - genetics | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Influenza A virus - classification - enzymology - genetics | en_HK |
dc.subject.mesh | Molecular Sequence Data | en_HK |
dc.subject.mesh | Polymerase Chain Reaction - methods | en_HK |
dc.subject.mesh | RNA Replicase - chemistry - genetics | en_HK |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction - methods | en_HK |
dc.subject.mesh | Viral Proteins - chemistry - genetics | en_HK |
dc.title | Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-0934&volume=142&issue=1-2&spage=218&epage=22&date=2007&atitle=Reliable+universal+RT-PCR+assays+for+studying+influenza+polymerase+subunit+gene+sequences+from+all+16+haemagglutinin+subtypes | en_HK |
dc.identifier.email | Leung, CYH: cyhleung@hkucc.hku.hk | en_HK |
dc.identifier.email | Chen, H: hlchen@hku.hk | en_HK |
dc.identifier.email | Guan, Y: yguan@hkucc.hku.hk | en_HK |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_HK |
dc.identifier.email | Poon, LLM: llmpoon@hkucc.hku.hk | en_HK |
dc.identifier.authority | Leung, CYH=rp00307 | en_HK |
dc.identifier.authority | Chen, H=rp00383 | en_HK |
dc.identifier.authority | Guan, Y=rp00397 | en_HK |
dc.identifier.authority | Peiris, JSM=rp00410 | en_HK |
dc.identifier.authority | Poon, LLM=rp00484 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jviromet.2007.01.015 | en_HK |
dc.identifier.pmid | 17324474 | - |
dc.identifier.scopus | eid_2-s2.0-34247163576 | en_HK |
dc.identifier.hkuros | 134612 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-34247163576&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 142 | en_HK |
dc.identifier.issue | 1-2 | en_HK |
dc.identifier.spage | 218 | en_HK |
dc.identifier.epage | 222 | en_HK |
dc.identifier.isi | WOS:000246675900029 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Li, OTW=16230718400 | en_HK |
dc.identifier.scopusauthorid | Barr, I=7004651416 | en_HK |
dc.identifier.scopusauthorid | Leung, CYH=26531438300 | en_HK |
dc.identifier.scopusauthorid | Chen, H=26643315400 | en_HK |
dc.identifier.scopusauthorid | Guan, Y=7202924055 | en_HK |
dc.identifier.scopusauthorid | Peiris, JSM=7005486823 | en_HK |
dc.identifier.scopusauthorid | Poon, LLM=7005441747 | en_HK |
dc.identifier.issnl | 0166-0934 | - |