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Article: Evaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin

TitleEvaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin
Authors
Wing Cheong YamMaria Li LungMun Hon Ng
Issue Date1992
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 1992, v. 30 n. 9, p. 2518-2520 How to Cite?
DOI: http://dx.doi.org/10.1128/JCM.30.9.2518-2520.1992
AbstractThe effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat- labile toxin) and 75% (for the detection of cholera toxin) to 100%, and the results were confirmed with bioassays and DNA hybridization assays for both clinical and environmental isolates.
Persistent Identifierhttp://hdl.handle.net/10722/78843
ISSN
0095-1137
2023 Impact Factor: 6.1
2023 SCImago Journal Rankings: 1.653
ISI Accession Number ID
WOS:A1992JJ48400061

 

DC FieldValueLanguage
dc.contributor.authorWing Cheong Yamen_HK
dc.contributor.authorMaria Li Lungen_HK
dc.contributor.authorMun Hon Ngen_HK
dc.date.accessioned2010-09-06T07:47:29Z-
dc.date.available2010-09-06T07:47:29Z-
dc.date.issued1992en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 1992, v. 30 n. 9, p. 2518-2520en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78843-
dc.description.abstractThe effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat- labile toxin) and 75% (for the detection of cholera toxin) to 100%, and the results were confirmed with bioassays and DNA hybridization assays for both clinical and environmental isolates.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.subject.meshAgglutination Tests - methodsen_HK
dc.subject.meshBacterial Toxins - analysis - immunologyen_HK
dc.subject.meshBiological Assayen_HK
dc.subject.meshCholera Toxin - analysis - immunologyen_HK
dc.subject.meshEnterotoxins - analysis - immunologyen_HK
dc.subject.meshEscherichia coli Proteinsen_HK
dc.subject.meshEvaluation Studies as Topicen_HK
dc.subject.meshImmunoassay - methodsen_HK
dc.subject.meshMicrospheresen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.titleEvaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=30&issue=9&spage=2518&epage=2520&date=1993&atitle=Evaluation+and+optimization+of+a+latex+agglutination+assay+for+detection+of+cholera+toxin+and+Escherichia+coli+heat-labile+toxinen_HK
dc.identifier.emailMaria Li Lung:mlilung@hku.hken_HK
dc.identifier.authorityMaria Li Lung=rp00300en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JCM.30.9.2518-2520.1992-
dc.identifier.pmid1401032-
dc.identifier.scopuseid_2-s2.0-0026768347en_HK
dc.identifier.hkuros61952en_HK
dc.identifier.volume30en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2518en_HK
dc.identifier.epage2520en_HK
dc.identifier.isiWOS:A1992JJ48400061-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWing Cheong Yam=7409818108en_HK
dc.identifier.scopusauthoridMaria Li Lung=7006411788en_HK
dc.identifier.scopusauthoridMun Hon Ng=7409939170en_HK
dc.identifier.issnl0095-1137-

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