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- Publisher Website: 10.1128/JVI.00735-09
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- PMID: 19494010
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Article: Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein
Title | Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein | ||||||||||||
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Authors | |||||||||||||
Issue Date | 2009 | ||||||||||||
Publisher | American Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/ | ||||||||||||
Citation | Journal Of Virology, 2009, v. 83 n. 16, p. 7850-7861 How to Cite? | ||||||||||||
Abstract | Interactions between host factors and the viral replication complex play important roles in host adaptation and regulation of influenza virus replication. A cellular protein, nuclear factor 90 (NF90), was copurified with H5N1 viral nucleoprotein (NP) from human cells in which NP was transiently expressed and identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. In vitro coimmunoprecipitation of NF90 and NP coexpressed in HEK 293T cells or individually expressed in bacterial and HEK 293T cells, respectively, confirmed a direct interaction between NF90 and NP, independent of other subunits of the ribonucleoprotein complex. This interaction was prevented by a mutation, F412A, in the C-terminal region of the NP, indicating that the C-terminal of NP is required for NF90 binding. RNase V treatment did not prevent coprecipitation of NP and NF90, which demonstrates that the interaction is RNA binding independent. After small interfering RNA knockdown of NF90 expression in A549 and HeLa cells, viral polymerase complex activity and virus replication were significantly increased, suggesting that NF90 negatively affects viral replication. Both NP and NF90 colocalized in the nucleus of virus-infected cells during the early phase of infection, suggesting that the interaction between NF90 and NP is an early event in virus replication. Quantitative reverse transcription-PCR showed that NF90 downregulates both viral genome replication and mRNA transcription in infected cells. These results suggest that NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP. Copyright © 2009, American Society for Microbiology. All Rights Reserved. | ||||||||||||
Persistent Identifier | http://hdl.handle.net/10722/59386 | ||||||||||||
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.378 | ||||||||||||
PubMed Central ID | |||||||||||||
ISI Accession Number ID |
Funding Information: This study was supported by the Research Grants Council of the Hong Kong Special Administrative Region (SAR), China (HKU 74888/05M and 7500/06M), the Area of Excellence Scheme of the University Grants Committee (grant AoE/M-12/06), the National Institutes of Health (NIAID contract HHSN2662007 00005C), the Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau of the Hong Kong SAR, and the Li Ka Shing Foundation. | ||||||||||||
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Grants |
DC Field | Value | Language |
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dc.contributor.author | Wang, P | en_HK |
dc.contributor.author | Song, W | en_HK |
dc.contributor.author | Mok, BWY | en_HK |
dc.contributor.author | Zhao, P | en_HK |
dc.contributor.author | Qin, K | en_HK |
dc.contributor.author | Lai, A | en_HK |
dc.contributor.author | Smith, GJD | en_HK |
dc.contributor.author | Zhang, J | en_HK |
dc.contributor.author | Lin, T | en_HK |
dc.contributor.author | Guan, Y | en_HK |
dc.contributor.author | Chen, H | en_HK |
dc.date.accessioned | 2010-05-31T03:49:03Z | - |
dc.date.available | 2010-05-31T03:49:03Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Journal Of Virology, 2009, v. 83 n. 16, p. 7850-7861 | en_HK |
dc.identifier.issn | 0022-538X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/59386 | - |
dc.description.abstract | Interactions between host factors and the viral replication complex play important roles in host adaptation and regulation of influenza virus replication. A cellular protein, nuclear factor 90 (NF90), was copurified with H5N1 viral nucleoprotein (NP) from human cells in which NP was transiently expressed and identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. In vitro coimmunoprecipitation of NF90 and NP coexpressed in HEK 293T cells or individually expressed in bacterial and HEK 293T cells, respectively, confirmed a direct interaction between NF90 and NP, independent of other subunits of the ribonucleoprotein complex. This interaction was prevented by a mutation, F412A, in the C-terminal region of the NP, indicating that the C-terminal of NP is required for NF90 binding. RNase V treatment did not prevent coprecipitation of NP and NF90, which demonstrates that the interaction is RNA binding independent. After small interfering RNA knockdown of NF90 expression in A549 and HeLa cells, viral polymerase complex activity and virus replication were significantly increased, suggesting that NF90 negatively affects viral replication. Both NP and NF90 colocalized in the nucleus of virus-infected cells during the early phase of infection, suggesting that the interaction between NF90 and NP is an early event in virus replication. Quantitative reverse transcription-PCR showed that NF90 downregulates both viral genome replication and mRNA transcription in infected cells. These results suggest that NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP. Copyright © 2009, American Society for Microbiology. All Rights Reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/ | en_HK |
dc.relation.ispartof | Journal of Virology | en_HK |
dc.rights | Journal of Virology. Copyright © American Society for Microbiology. | en_HK |
dc.rights | Copyright © American Society for Microbiology, Journal of Virology, 2009, v. 83 n. 16, p. 7850-7861 | - |
dc.subject.mesh | Down-Regulation | - |
dc.subject.mesh | Influenza A Virus, H5N1 Subtype - genetics - physiology | - |
dc.subject.mesh | Influenza, Human - metabolism - virology | - |
dc.subject.mesh | Nuclear Factor 90 Proteins - chemistry - genetics - metabolism | - |
dc.subject.mesh | Nucleoproteins - genetics - metabolism | - |
dc.title | Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=83&spage=7850&epage=61&date=2009&atitle=Nuclear+factor+90,+NF90,+negatively+regulates+influenza+virus+replication+by+interacting+with+viral+nucleoprotein | en_HK |
dc.identifier.email | Smith, GJD: gjsmith@hkucc1.hku.hk | en_HK |
dc.identifier.email | Zhang, J: zhangajx@hkucc.hku.hk | en_HK |
dc.identifier.email | Guan, Y: yguan@hkucc.hku.hk | en_HK |
dc.identifier.email | Chen, H: hlchen@hku.hk | en_HK |
dc.identifier.authority | Smith, GJD=rp00444 | en_HK |
dc.identifier.authority | Zhang, J=rp00413 | en_HK |
dc.identifier.authority | Guan, Y=rp00397 | en_HK |
dc.identifier.authority | Chen, H=rp00383 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1128/JVI.00735-09 | en_HK |
dc.identifier.pmid | 19494010 | - |
dc.identifier.pmcid | PMC2715781 | - |
dc.identifier.scopus | eid_2-s2.0-67749127718 | en_HK |
dc.identifier.hkuros | 160812 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-67749127718&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 83 | en_HK |
dc.identifier.issue | 16 | en_HK |
dc.identifier.spage | 7850 | en_HK |
dc.identifier.epage | 7861 | en_HK |
dc.identifier.eissn | 1098-5514 | - |
dc.identifier.isi | WOS:000268208700006 | - |
dc.publisher.place | United States | en_HK |
dc.relation.project | Control of Pandemic and Inter-pandemic Influenza | - |
dc.identifier.scopusauthorid | Wang, P=13907209900 | en_HK |
dc.identifier.scopusauthorid | Song, W=23490429800 | en_HK |
dc.identifier.scopusauthorid | Mok, BWY=36786012800 | en_HK |
dc.identifier.scopusauthorid | Zhao, P=34972423500 | en_HK |
dc.identifier.scopusauthorid | Qin, K=8512090900 | en_HK |
dc.identifier.scopusauthorid | Lai, A=36769811700 | en_HK |
dc.identifier.scopusauthorid | Smith, GJD=8344015800 | en_HK |
dc.identifier.scopusauthorid | Zhang, J=12752135600 | en_HK |
dc.identifier.scopusauthorid | Lin, T=7404860987 | en_HK |
dc.identifier.scopusauthorid | Guan, Y=7202924055 | en_HK |
dc.identifier.scopusauthorid | Chen, H=26643315400 | en_HK |
dc.identifier.citeulike | 4768934 | - |
dc.identifier.issnl | 0022-538X | - |