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Article: Isolation and Detection of Proteins with Nano-Particles and Microchips for Analyzing Proteomes on a Large Scale Basis
| Title | Isolation and Detection of Proteins with Nano-Particles and Microchips for Analyzing Proteomes on a Large Scale Basis |
|---|---|
| Authors | |
| Keywords | Capillary electrochromatography Lab-on-a-chip Micro-total-analysis systems Nanoparticles |
| Issue Date | 1-Apr-2011 |
| Publisher | Bentham Science Publishers |
| Citation | Protein and Peptide Letters, 2011, v. 18, n. 4, p. 423-433 How to Cite? |
| Abstract | The advent of sugar-immobilized gold nano-particles (SGNPs), lipid-based nanoparticles, nanochromatography and nano-electrophoresis has revolutionized the methodology for proteins purification and proteomic research. This review provides an overview on the effective method developed for fast purification of proteins from extracts using SGNPs. In addition, the current application of microfluidic systems for analytical purposes in biochemistry will also be explored that include the micro total analysis systems (μ-TAS) and lab-on-a-chip (LOC) analyses which are capable of isolation and detection of proteins at the nanogram level. Finally, we describe why the lipid-based nano-particles (LBNPs) can enable the analysis in microchip electroseparation and how anionic and cationic LBNPs can be used for proteins separation. |
| Persistent Identifier | http://hdl.handle.net/10722/358342 |
| ISSN | 2023 Impact Factor: 1.0 2023 SCImago Journal Rankings: 0.349 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Chan, Ki | - |
| dc.contributor.author | Ng, Tzi Bun | - |
| dc.date.accessioned | 2025-08-06T00:30:04Z | - |
| dc.date.available | 2025-08-06T00:30:04Z | - |
| dc.date.issued | 2011-04-01 | - |
| dc.identifier.citation | Protein and Peptide Letters, 2011, v. 18, n. 4, p. 423-433 | - |
| dc.identifier.issn | 0929-8665 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/358342 | - |
| dc.description.abstract | <p>The advent of sugar-immobilized gold nano-particles (SGNPs), lipid-based nanoparticles, nanochromatography and nano-electrophoresis has revolutionized the methodology for proteins purification and proteomic research. This review provides an overview on the effective method developed for fast purification of proteins from extracts using SGNPs. In addition, the current application of microfluidic systems for analytical purposes in biochemistry will also be explored that include the micro total analysis systems (μ-TAS) and lab-on-a-chip (LOC) analyses which are capable of isolation and detection of proteins at the nanogram level. Finally, we describe why the lipid-based nano-particles (LBNPs) can enable the analysis in microchip electroseparation and how anionic and cationic LBNPs can be used for proteins separation.<br></p> | - |
| dc.language | eng | - |
| dc.publisher | Bentham Science Publishers | - |
| dc.relation.ispartof | Protein and Peptide Letters | - |
| dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
| dc.subject | Capillary electrochromatography | - |
| dc.subject | Lab-on-a-chip | - |
| dc.subject | Micro-total-analysis systems | - |
| dc.subject | Nanoparticles | - |
| dc.title | Isolation and Detection of Proteins with Nano-Particles and Microchips for Analyzing Proteomes on a Large Scale Basis | - |
| dc.type | Article | - |
| dc.identifier.doi | 10.2174/092986611794653978 | - |
| dc.identifier.scopus | eid_2-s2.0-79951657926 | - |
| dc.identifier.volume | 18 | - |
| dc.identifier.issue | 4 | - |
| dc.identifier.spage | 423 | - |
| dc.identifier.epage | 433 | - |
| dc.identifier.eissn | 1875-5305 | - |
| dc.identifier.issnl | 0929-8665 | - |