Concurrently Probing the Mechanical and Electrical Characteristics of Living Cells via an Integrated Microdevice

Abstract

The mechanical and electrical properties of cells serve as critical indicators of their physiological and pathological state. Currently, distinct setups are required to measure the electrical and mechanical responses of cells. In addition, most existing methods such as optical trapping (OT) and atomic force microscopy (AFM) are labor-intensive, expensive, and low-throughput. Here, we developed a microdevice that integrates automated cell trapping, deformation, and electric impedance spectroscopy to overcome these limitations. Our device enables parallel aspiration of tens of trapped cells in a highly scalable manner by simply adjusting the applied pressures, allowing for rapid probing of the dynamic viscoelastic properties of cells. Furthermore, embedded microelectrodes enable concurrent investigations of the electrical impedance of the cells. Through testing on different cell types, our platform demonstrated superior capabilities in comprehensive cell characterization and phenotyping, highlighting its great potential as a versatile tool for single cell analysis, drug screening, and disease detection.