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Article: Bacterial Cytochrome P450 Catalyzed Post‐translational Macrocyclization of Ribosomal Peptides

TitleBacterial Cytochrome P450 Catalyzed Post‐translational Macrocyclization of Ribosomal Peptides
Authors
Issue Date28-Sep-2023
PublisherWiley
Citation
Angewandte Chemie International Edition, 2023 How to Cite?
Abstract

Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a fascinating group of natural products that exhibit diverse structural features and bioactivities. P450-catalyzed RiPPs stand out as a unique but underexplored family. Here, we introduce a rule-based genome mining strategy that harnesses the intrinsic biosynthetic principles of RiPPs, including the co-occurrence, co-conservation, and interactions between precursors and P450s, successfully facilitating the identification of diverse P450 catalyzed RiPPs. Intensive BGC characterization revealed four new P450s, KstB, ScnB, MciB, and SgrB, that can respectively catalyze Trp-Trp-Tyr (one C-C and two C-N bonds), Tyr-Trp (C-C bond), Trp-Trp (C-N bond), and His-His (ether bond) crosslinks within three or four residues. KstB, ScnB, and MciB could accept non-native precursors, suggesting they could be promising starting templates for bioengineering to construct macrocycles. Our study highlights the potential of P450s in expanding the chemical diversity of strained macrocyclic peptides and enriching biocatalytic tools for peptide macrocyclization.


Persistent Identifierhttp://hdl.handle.net/10722/337394
ISSN
2023 Impact Factor: 16.1
2023 SCImago Journal Rankings: 5.300

 

DC FieldValueLanguage
dc.contributor.authorHe, Bei-Bei-
dc.contributor.authorLiu, Jing-
dc.contributor.authorCheng, Zhuo-
dc.contributor.authorLiu, Runze-
dc.contributor.authorZhong, Zheng-
dc.contributor.authorGao, Ying-
dc.contributor.authorLiu, Hongyan-
dc.contributor.authorSong, Zhi-Man-
dc.contributor.authorTian, Yongqi-
dc.contributor.authorLi, Yong-Xin-
dc.contributor.authorLiu, Jing-
dc.contributor.authorCheng, Zhuo-
dc.contributor.authorLiu, Runze-
dc.contributor.authorZhong, Zheng-
dc.contributor.authorGao, Ying-
dc.contributor.authorLiu, Hongyan-
dc.contributor.authorSong, Zhi-Man-
dc.contributor.authorTian, Yongqi-
dc.contributor.authorLi, Yong-Xin -
dc.date.accessioned2024-03-11T10:20:32Z-
dc.date.available2024-03-11T10:20:32Z-
dc.date.issued2023-09-28-
dc.identifier.citationAngewandte Chemie International Edition, 2023-
dc.identifier.issn1433-7851-
dc.identifier.urihttp://hdl.handle.net/10722/337394-
dc.description.abstract<p>Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a fascinating group of natural products that exhibit diverse structural features and bioactivities. P450-catalyzed RiPPs stand out as a unique but underexplored family. Here, we introduce a rule-based genome mining strategy that harnesses the intrinsic biosynthetic principles of RiPPs, including the co-occurrence, co-conservation, and interactions between precursors and P450s, successfully facilitating the identification of diverse P450 catalyzed RiPPs. Intensive BGC characterization revealed four new P450s, KstB, ScnB, MciB, and SgrB, that can respectively catalyze Trp-Trp-Tyr (one C-C and two C-N bonds), Tyr-Trp (C-C bond), Trp-Trp (C-N bond), and His-His (ether bond) crosslinks within three or four residues. KstB, ScnB, and MciB could accept non-native precursors, suggesting they could be promising starting templates for bioengineering to construct macrocycles. Our study highlights the potential of P450s in expanding the chemical diversity of strained macrocyclic peptides and enriching biocatalytic tools for peptide macrocyclization.<br></p>-
dc.languageeng-
dc.publisherWiley-
dc.relation.ispartofAngewandte Chemie International Edition-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleBacterial Cytochrome P450 Catalyzed Post‐translational Macrocyclization of Ribosomal Peptides-
dc.typeArticle-
dc.identifier.doi10.1002/anie.202311533-
dc.identifier.eissn1521-3773-
dc.identifier.issnl1433-7851-

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