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Article: APLNR marks a cardiac progenitor derived with human induced pluripotent stem cells

TitleAPLNR marks a cardiac progenitor derived with human induced pluripotent stem cells
Authors
KeywordsCardiac stem cell biology
Induced pluripotent stem cells
Pluripotent stem cell derived cardiomyocytes
Stem cell differentiation
Issue Date1-Jul-2023
PublisherElsevier
Citation
Heliyon, 2023, v. 9, n. 7 How to Cite?
Abstract

Cardiomyocytes can be readily derived from human induced pluripotent stem cell (hiPSC) lines,
yet its efficacy varies across different batches of the same and different hiPSC lines. To unravel the
inconsistencies of in vitro cardiac differentiation, we utilized single cell transcriptomics on hiPSCs
undergoing cardiac differentiation and identified cardiac and extra-cardiac lineages throughout
differentiation. We further identified APLNR as a surface marker for in vitro cardiac progenitors
and immunomagnetically isolated them. Differentiation of isolated in vitro APLNR+ cardiac
progenitors derived from multiple hiPSC lines resulted in predominantly cardiomyocytes
accompanied with cardiac mesenchyme. Transcriptomic analysis of differentiating in vitro
APLNR+ cardiac progenitors revealed transient expression of cardiac progenitor markers before
further commitment into cardiomyocyte and cardiac mesenchyme. Analysis of in vivo human and
mouse embryo single cell transcriptomic datasets have identified APLNR expression in early
cardiac progenitors of multiple lineages. This platform enables generation of in vitro cardiac
progenitors from multiple hiPSC lines without genetic manipulation, which has potential applications
in studying cardiac development, disease modelling and cardiac regeneration.


Persistent Identifierhttp://hdl.handle.net/10722/337067
ISSN
2023 Impact Factor: 3.4
2023 SCImago Journal Rankings: 0.617
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLam, Yin Yu-
dc.contributor.authorChan, Chun Ho-
dc.contributor.authorGeng, Lin-
dc.contributor.authorWong, Nicodemus-
dc.contributor.authorKeung, Wendy-
dc.contributor.authorCheung, Yiu Fai-
dc.date.accessioned2024-03-11T10:17:50Z-
dc.date.available2024-03-11T10:17:50Z-
dc.date.issued2023-07-01-
dc.identifier.citationHeliyon, 2023, v. 9, n. 7-
dc.identifier.issn2405-8440-
dc.identifier.urihttp://hdl.handle.net/10722/337067-
dc.description.abstract<p>Cardiomyocytes can be readily derived from human induced pluripotent stem cell (hiPSC) lines,<br>yet its efficacy varies across different batches of the same and different hiPSC lines. To unravel the<br>inconsistencies of in vitro cardiac differentiation, we utilized single cell transcriptomics on hiPSCs<br>undergoing cardiac differentiation and identified cardiac and extra-cardiac lineages throughout<br>differentiation. We further identified APLNR as a surface marker for in vitro cardiac progenitors<br>and immunomagnetically isolated them. Differentiation of isolated in vitro APLNR+ cardiac<br>progenitors derived from multiple hiPSC lines resulted in predominantly cardiomyocytes<br>accompanied with cardiac mesenchyme. Transcriptomic analysis of differentiating in vitro<br>APLNR+ cardiac progenitors revealed transient expression of cardiac progenitor markers before<br>further commitment into cardiomyocyte and cardiac mesenchyme. Analysis of in vivo human and<br>mouse embryo single cell transcriptomic datasets have identified APLNR expression in early<br>cardiac progenitors of multiple lineages. This platform enables generation of in vitro cardiac<br>progenitors from multiple hiPSC lines without genetic manipulation, which has potential applications<br>in studying cardiac development, disease modelling and cardiac regeneration.<br></p>-
dc.languageeng-
dc.publisherElsevier-
dc.relation.ispartofHeliyon-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCardiac stem cell biology-
dc.subjectInduced pluripotent stem cells-
dc.subjectPluripotent stem cell derived cardiomyocytes-
dc.subjectStem cell differentiation-
dc.titleAPLNR marks a cardiac progenitor derived with human induced pluripotent stem cells-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.heliyon.2023.e18243-
dc.identifier.scopuseid_2-s2.0-85166332556-
dc.identifier.volume9-
dc.identifier.issue7-
dc.identifier.eissn2405-8440-
dc.identifier.isiWOS:001039955600001-
dc.identifier.issnl2405-8440-

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