The Intrahepatic Presence Of The Specific Bacterial Species Is Associated With The Alleviation Of Hepatic Steatosis In A Human MicrobiotaAssociated Murine Model

Abstract

Background The Occurrence Of Metabolic Dysfunction-Associated Fatty Liver Disease (Mafld), Highly Associated With Obesity, Is Not Rare In Lean People. To Date, It Has Been Discovered That Bacterial Dna Is Present In The Liver Of Mafld Patients. Whether There Are Differential Intrahepatic Bacterial Profiles In Lean And Obese Mafld, And Their Functional Roles Are Yet Uncertain. Methods A Human Microbiota-Associated (Hma) Murine Model Was Established By Transplanting Fecal Microbiota Into C57bl/6j Mice Individually Using Fecal Slurry From Lean And Obese Mafld Donors (N=8 In Each Group). 16s Rrna Sequencing On V3-V4 Regions Was Used To Target The Bacterial Dna Extracted From The Liver Of Hma Mice. Microbiome Analysis On Bacterial Composition At Different Taxonomic Levels And The Microbial Functional Prediction Was Performed. Results Lean Recipient Mice Had Significantly Lower Intrahepatic Triglyceride Content (46.89±3.86 Mg/G Vs. 68.51±4.18 Mg/G, P=0.002) And Microbial Diversity (Chao 1 Richness: 207.00±15.21 Vs. 282.20±16.03, P=0.002) Than Obese Recipient Mice. Biomarker Discovery Analysis Showed That Streptococcus Oralis (Log₁₀ Lda Score 4,80, P=0.002) And Bacteroides Acidifaciens (Log₁₀ Lda Score 4.75, P=0.001) Were Significantly Enriched In The Liver Of Lean Recipient Mice When Compared To Obese Recipient Mice At The Species Level, Which All Correlated Negatively With Intrahepatic Triglyceride Levels (R=-0.755, P=0.001 And R=- 0.801, P<0.001). The Predicted Flavin Biosynthesis I Pathway Was Significantly Enriched In Lean Recipient Mice When Compared To Obese Recipient Mice With The Highest Log₁₀ Lda Score Of 4.83 (P=0.046); And Was Correlated Positively With Bacteroides Acidifaciens (R=0.557, P=0.025). Conclusion The Hma Mafld Murine Model Demonstrated That Intrahepatic Bacterial Abundance Might Be Associated With Intrahepatic Triglycerides. The Presence Of Bacteroides Acidifaciens In The Liver, Previously Known For Beneficial Effects On Obesity, May Play A Beneficial Role In Preventing Hepatic Steatosis In Part Through The Mediation Of Riboflavin Production.