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Article: A single-cell transcriptome of mesenchymal stromal cells to fabricate bioactive hydroxyapatite materials for bone regeneration

TitleA single-cell transcriptome of mesenchymal stromal cells to fabricate bioactive hydroxyapatite materials for bone regeneration
Authors
KeywordsSingle-cell RNA sequencing
Bone regeneration microenvironment
MSC heterogeneity
PCDH10
Bone tissue engineering
Issue Date2022
PublisherElsevier B.V. on behalf of KeAi Communications Co. Ltd. The Journal's web site is located at http://www.sciencedirect.com/science/journal/2452199X
Citation
Bioactive Materials, 2022, v. 9, p. 281-298 How to Cite?
AbstractThe osteogenic microenvironment of bone-repairing materials plays a key role in accelerating bone regeneration but remains incompletely defined, which significantly limits the application of such bioactive materials. Here, the transcriptional landscapes of different osteogenic microenvironments, including three-dimensional (3D) hydroxyapatite (HA) scaffolds and osteogenic medium (OM), for mesenchymal stromal cells (MSCs) in vitro were mapped at single-cell resolution. Our findings suggested that an osteogenic process reminiscent of endochondral ossification occurred in HA scaffolds through sequential activation of osteogenic-related signaling pathways, along with inflammation and angiogenesis, but inhibition of adipogenesis and fibrosis. Moreover, we revealed the mechanism during OM-mediated osteogenesis involves the ZBTB16 and WNT signaling pathways. Heterogeneity of MSCs was also demonstrated. In vitro ossification of LRRC75A+ MSCs was shown to have better utilization of WNT-related ossification process, and PCDH10+ MSCs with superiority in hydroxyapatite-related osteogenic process. These findings provided further understanding of the cellular activity modulated by OM conditions and HA scaffolds, providing new insights for the improvement of osteogenic biomaterials. This atlas provides a blueprint for research on MSC heterogeneity and the osteogenic microenvironment of HA scaffolds and a database reference for the application of bioactive materials for bone regeneration.
Persistent Identifierhttp://hdl.handle.net/10722/305741
ISSN
2023 Impact Factor: 18.0
2023 SCImago Journal Rankings: 3.466
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGuo, P-
dc.contributor.authorLiu, X-
dc.contributor.authorZhang, P-
dc.contributor.authorHe, Z-
dc.contributor.authorLi, Z-
dc.contributor.authorAlini, M-
dc.contributor.authorRichards, RG-
dc.contributor.authorGrad, S-
dc.contributor.authorStoddart, MJ-
dc.contributor.authorZhou, G-
dc.contributor.authorZou, X-
dc.contributor.authorChan, D-
dc.contributor.authorTian, W-
dc.contributor.authorChen, D-
dc.contributor.authorGao, M-
dc.contributor.authorZhou, Z-
dc.contributor.authorLiu, S-
dc.date.accessioned2021-10-20T10:13:40Z-
dc.date.available2021-10-20T10:13:40Z-
dc.date.issued2022-
dc.identifier.citationBioactive Materials, 2022, v. 9, p. 281-298-
dc.identifier.issn2452-199X-
dc.identifier.urihttp://hdl.handle.net/10722/305741-
dc.description.abstractThe osteogenic microenvironment of bone-repairing materials plays a key role in accelerating bone regeneration but remains incompletely defined, which significantly limits the application of such bioactive materials. Here, the transcriptional landscapes of different osteogenic microenvironments, including three-dimensional (3D) hydroxyapatite (HA) scaffolds and osteogenic medium (OM), for mesenchymal stromal cells (MSCs) in vitro were mapped at single-cell resolution. Our findings suggested that an osteogenic process reminiscent of endochondral ossification occurred in HA scaffolds through sequential activation of osteogenic-related signaling pathways, along with inflammation and angiogenesis, but inhibition of adipogenesis and fibrosis. Moreover, we revealed the mechanism during OM-mediated osteogenesis involves the ZBTB16 and WNT signaling pathways. Heterogeneity of MSCs was also demonstrated. In vitro ossification of LRRC75A+ MSCs was shown to have better utilization of WNT-related ossification process, and PCDH10+ MSCs with superiority in hydroxyapatite-related osteogenic process. These findings provided further understanding of the cellular activity modulated by OM conditions and HA scaffolds, providing new insights for the improvement of osteogenic biomaterials. This atlas provides a blueprint for research on MSC heterogeneity and the osteogenic microenvironment of HA scaffolds and a database reference for the application of bioactive materials for bone regeneration.-
dc.languageeng-
dc.publisherElsevier B.V. on behalf of KeAi Communications Co. Ltd. The Journal's web site is located at http://www.sciencedirect.com/science/journal/2452199X-
dc.relation.ispartofBioactive Materials-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectSingle-cell RNA sequencing-
dc.subjectBone regeneration microenvironment-
dc.subjectMSC heterogeneity-
dc.subjectPCDH10-
dc.subjectBone tissue engineering-
dc.titleA single-cell transcriptome of mesenchymal stromal cells to fabricate bioactive hydroxyapatite materials for bone regeneration-
dc.typeArticle-
dc.identifier.emailChan, D: chand@hku.hk-
dc.identifier.authorityChan, D=rp00540-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.bioactmat.2021.08.009-
dc.identifier.pmid34820571-
dc.identifier.pmcidPMC8586438-
dc.identifier.scopuseid_2-s2.0-85112531841-
dc.identifier.hkuros327873-
dc.identifier.volume9-
dc.identifier.spage281-
dc.identifier.epage298-
dc.identifier.isiWOS:000736149300002-
dc.publisher.placeChina-

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