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- Publisher Website: 10.1038/s41541-021-00359-8
- Scopus: eid_2-s2.0-85111989018
- PMID: 34349128
- WOS: WOS:000681310800001
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Article: Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges
Title | Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
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Authors | |
Issue Date | 2021 |
Publisher | Nature Research: Fully Open Access Journals. The Journal's web site is located at http://www.nature.com/npjvaccines/ |
Citation | NPJ Vaccines, 2021, v. 6, p. article no. 95 How to Cite? |
Abstract | We previously developed a temperature-sensitive, and NS1 gene deleted live attenuated influenza vaccine (DelNS1-LAIV) and demonstrated its potent protective efficacy in intranasally vaccinated mice. Here we investigated whether intradermal (i.d.) vaccination induces protective immunity. Our results showed that DelNS1-LAIV intradermal vaccination conferred effective and long-lasting protection against lethal virus challenge in mice. A single intradermal injection of DelNS1-LAIV conferred 100% survival with no weight loss in mice after A(H1N1)09 influenza virus (H1N1/415742Md) challenge. DelNS1-LAIV injection resulted in a significant reduction of lung viral load and reduced airway epithelial cell death and lung inflammatory cytokine responses at day 2 and 4 post challenge. Full protections of mice lasted for 6 months after immunization. In vitro infection of DelNS1-LAIV in monocyte-derived dendritic cells (MoDCs) demonstrated activation of antigen-presenting cells at 33 °C, together with the results of abortive replication of DelNS1-LAIV in skin tissue and strong upregulation of inflammatory cytokines/chemokines expression, our results suggested the strong immunogenicity of this vaccine. Further, we demonstrate that the underlying protection mechanism induced by intradermal DelNS1-LAIV is mainly attributed to antibody responses. Together, this study opens up an alternative route for the administration of LAIV, which may benefit individuals not suitable for intranasal LAIV immunization. |
Persistent Identifier | http://hdl.handle.net/10722/304268 |
ISSN | 2023 Impact Factor: 6.9 2023 SCImago Journal Rankings: 2.127 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lee, ACY | - |
dc.contributor.author | Zhang, AJ | - |
dc.contributor.author | Li, C | - |
dc.contributor.author | Chen, Y | - |
dc.contributor.author | Liu, F | - |
dc.contributor.author | Zhao, Y | - |
dc.contributor.author | Chu, H | - |
dc.contributor.author | Fong, CHY | - |
dc.contributor.author | Wang, P | - |
dc.contributor.author | Lau, SY | - |
dc.contributor.author | To, KKW | - |
dc.contributor.author | Chen, H | - |
dc.contributor.author | Yuen, KY | - |
dc.date.accessioned | 2021-09-23T08:57:38Z | - |
dc.date.available | 2021-09-23T08:57:38Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | NPJ Vaccines, 2021, v. 6, p. article no. 95 | - |
dc.identifier.issn | 2059-0105 | - |
dc.identifier.uri | http://hdl.handle.net/10722/304268 | - |
dc.description.abstract | We previously developed a temperature-sensitive, and NS1 gene deleted live attenuated influenza vaccine (DelNS1-LAIV) and demonstrated its potent protective efficacy in intranasally vaccinated mice. Here we investigated whether intradermal (i.d.) vaccination induces protective immunity. Our results showed that DelNS1-LAIV intradermal vaccination conferred effective and long-lasting protection against lethal virus challenge in mice. A single intradermal injection of DelNS1-LAIV conferred 100% survival with no weight loss in mice after A(H1N1)09 influenza virus (H1N1/415742Md) challenge. DelNS1-LAIV injection resulted in a significant reduction of lung viral load and reduced airway epithelial cell death and lung inflammatory cytokine responses at day 2 and 4 post challenge. Full protections of mice lasted for 6 months after immunization. In vitro infection of DelNS1-LAIV in monocyte-derived dendritic cells (MoDCs) demonstrated activation of antigen-presenting cells at 33 °C, together with the results of abortive replication of DelNS1-LAIV in skin tissue and strong upregulation of inflammatory cytokines/chemokines expression, our results suggested the strong immunogenicity of this vaccine. Further, we demonstrate that the underlying protection mechanism induced by intradermal DelNS1-LAIV is mainly attributed to antibody responses. Together, this study opens up an alternative route for the administration of LAIV, which may benefit individuals not suitable for intranasal LAIV immunization. | - |
dc.language | eng | - |
dc.publisher | Nature Research: Fully Open Access Journals. The Journal's web site is located at http://www.nature.com/npjvaccines/ | - |
dc.relation.ispartof | NPJ Vaccines | - |
dc.rights | NPJ Vaccines. Copyright © Nature Research: Fully Open Access Journals. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges | - |
dc.type | Article | - |
dc.identifier.email | Lee, ACY: cyalee@hku.hk | - |
dc.identifier.email | Zhang, AJ: zhangajx@hkucc.hku.hk | - |
dc.identifier.email | Li, C: canlee@hku.hk | - |
dc.identifier.email | Liu, F: liuts89@HKUCC-COM.hku.hk | - |
dc.identifier.email | Zhao, Y: zoezhao7@hku.hk | - |
dc.identifier.email | Chu, H: hinchu@hku.hk | - |
dc.identifier.email | Fong, CHY: chyfong@hku.hk | - |
dc.identifier.email | Wang, P: puiwang@hkucc.hku.hk | - |
dc.identifier.email | To, KKW: kelvinto@hku.hk | - |
dc.identifier.email | Chen, H: hlchen@hku.hk | - |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | - |
dc.identifier.authority | Zhang, AJ=rp00413 | - |
dc.identifier.authority | Chu, H=rp02125 | - |
dc.identifier.authority | To, KKW=rp01384 | - |
dc.identifier.authority | Chen, H=rp00383 | - |
dc.identifier.authority | Yuen, KY=rp00366 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1038/s41541-021-00359-8 | - |
dc.identifier.pmid | 34349128 | - |
dc.identifier.pmcid | PMC8339132 | - |
dc.identifier.scopus | eid_2-s2.0-85111989018 | - |
dc.identifier.hkuros | 325633 | - |
dc.identifier.volume | 6 | - |
dc.identifier.spage | article no. 95 | - |
dc.identifier.epage | article no. 95 | - |
dc.identifier.isi | WOS:000681310800001 | - |
dc.publisher.place | United Kingdom | - |