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Article: Salivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome

TitleSalivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome
Authors
KeywordsSaliva
bacteriome
oral microbiome
leukoplakia
oral cancer
Issue Date2021
PublisherTaylor & Francis: Open Access Journals. The Journal's web site is located at http://www.journaloforalmicrobiology.net
Citation
Journal of Oral Microbiology, 2021, v. 13 n. 1, p. article no. 1857998 How to Cite?
AbstractObjective: While some oral carcinomas appear to arise de novo, others develop within long-standing conditions of the oral cavity that have malignant potential, now known as oral potentially malignant disorders (OPMDs). The oral bacteriome associated with OPMD has been studied to a lesser extent than that associated with oral cancer. To characterize the association in detail we compared the bacteriome in whole mouth fluid (WMF) in patients with oral leukoplakia, oral cancer and healthy controls. Methods: WMF bacteriome from 20 leukoplakia patients, 31 patients with oral cancer and 23 healthy controls were profiled using the Illumina MiSeq platform. Sequencing reads were processed using DADA2, and taxonomical classification was performed using the phylogenetic placement method. Sparse Partial Least Squares Regression Discriminant Analysis model was used to identify bacterial taxa that best discriminate the studied groups. Results: We found considerable overlap between the WMF bacteriome of leukoplakia and oral cancer while a clearer separation between healthy controls and the former two disorders was observed. Specifically, the separation was attributed to 14 taxa belonging to the genera Megaspheara, unclassified enterobacteria, Prevotella, Porphyromonas, Rothia and Salmonella, Streptococcus, and Fusobacterium. The most discriminative bacterial genera between leukoplakia and oral cancer were Megasphaera, unclassified Enterobacteriae, Salmonella and Prevotella. Conclusion: Oral bacteria may play a role in the early stages of oral carcinogenesis as a dysbiotic bacteriome is associated with oral leukoplakia and this resembles that of oral cancer more than healthy controls. Our findings may have implications for developing oral cancer prevention strategies targeting early microbial drivers of oral carcinogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/298747
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 0.919
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGOPINATH, D-
dc.contributor.authorKUNNATH MENON, R-
dc.contributor.authorCHUN WIE, C-
dc.contributor.authorBANERJEE, M-
dc.contributor.authorPANDA, S-
dc.contributor.authorMANDAL, D-
dc.contributor.authorBEHERA, PK-
dc.contributor.authorROYCHOUDHURY, S-
dc.contributor.authorKHEUR, S-
dc.contributor.authorBotelho, MG-
dc.contributor.authorJohnson, NW-
dc.date.accessioned2021-04-12T03:02:49Z-
dc.date.available2021-04-12T03:02:49Z-
dc.date.issued2021-
dc.identifier.citationJournal of Oral Microbiology, 2021, v. 13 n. 1, p. article no. 1857998-
dc.identifier.issn2000-2297-
dc.identifier.urihttp://hdl.handle.net/10722/298747-
dc.description.abstractObjective: While some oral carcinomas appear to arise de novo, others develop within long-standing conditions of the oral cavity that have malignant potential, now known as oral potentially malignant disorders (OPMDs). The oral bacteriome associated with OPMD has been studied to a lesser extent than that associated with oral cancer. To characterize the association in detail we compared the bacteriome in whole mouth fluid (WMF) in patients with oral leukoplakia, oral cancer and healthy controls. Methods: WMF bacteriome from 20 leukoplakia patients, 31 patients with oral cancer and 23 healthy controls were profiled using the Illumina MiSeq platform. Sequencing reads were processed using DADA2, and taxonomical classification was performed using the phylogenetic placement method. Sparse Partial Least Squares Regression Discriminant Analysis model was used to identify bacterial taxa that best discriminate the studied groups. Results: We found considerable overlap between the WMF bacteriome of leukoplakia and oral cancer while a clearer separation between healthy controls and the former two disorders was observed. Specifically, the separation was attributed to 14 taxa belonging to the genera Megaspheara, unclassified enterobacteria, Prevotella, Porphyromonas, Rothia and Salmonella, Streptococcus, and Fusobacterium. The most discriminative bacterial genera between leukoplakia and oral cancer were Megasphaera, unclassified Enterobacteriae, Salmonella and Prevotella. Conclusion: Oral bacteria may play a role in the early stages of oral carcinogenesis as a dysbiotic bacteriome is associated with oral leukoplakia and this resembles that of oral cancer more than healthy controls. Our findings may have implications for developing oral cancer prevention strategies targeting early microbial drivers of oral carcinogenesis.-
dc.languageeng-
dc.publisherTaylor & Francis: Open Access Journals. The Journal's web site is located at http://www.journaloforalmicrobiology.net-
dc.relation.ispartofJournal of Oral Microbiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectSaliva-
dc.subjectbacteriome-
dc.subjectoral microbiome-
dc.subjectleukoplakia-
dc.subjectoral cancer-
dc.titleSalivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome-
dc.typeArticle-
dc.identifier.emailBotelho, MG: botelho@hkucc.hku.hk-
dc.identifier.authorityBotelho, MG=rp00033-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1080/20002297.2020.1857998-
dc.identifier.pmid33391629-
dc.identifier.pmcidPMC7734041-
dc.identifier.scopuseid_2-s2.0-85097387814-
dc.identifier.hkuros321990-
dc.identifier.volume13-
dc.identifier.issue1-
dc.identifier.spagearticle no. 1857998-
dc.identifier.epagearticle no. 1857998-
dc.identifier.isiWOS:000596861100001-
dc.publisher.placeUnited Kingdom-

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