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Article: Evaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat, and hamster sera

TitleEvaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat, and hamster sera
Authors
Perera, RAPMKo, RTsang, OTYHui, DSCKwan, MYMBrackman, CJTo, EMWYen, HLLeung, KCheng, SMSChan, KHChan, KCKLi, KCSaif, LBarrs, VRWu, JTSit, THCPoon, LLMPeiris, M
KeywordsSARS coronavirus 2
COVID-19
serology
neutralization
seroepidemiology
Issue Date2020
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/
Citation
Journal of Clinical Microbiology, 2020, v. 59 n. 2, p. article no. e02504-20 How to Cite?
DOI: http://dx.doi.org/10.1128/JCM.02504-20
AbstractSurrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat, and hamster sera. With PRNT90 as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT90, except for cross-reactivity to SARS-CoV-1 in sVNT.
Persistent Identifierhttp://hdl.handle.net/10722/295231
ISSN
0095-1137
2023 Impact Factor: 6.1
2023 SCImago Journal Rankings: 1.653
PubMed Central ID
PMC8111130
ISI Accession Number ID
WOS:000610235700037

 

DC FieldValueLanguage
dc.contributor.authorPerera, RAPM-
dc.contributor.authorKo, R-
dc.contributor.authorTsang, OTY-
dc.contributor.authorHui, DSC-
dc.contributor.authorKwan, MYM-
dc.contributor.authorBrackman, CJ-
dc.contributor.authorTo, EMW-
dc.contributor.authorYen, HL-
dc.contributor.authorLeung, K-
dc.contributor.authorCheng, SMS-
dc.contributor.authorChan, KH-
dc.contributor.authorChan, KCK-
dc.contributor.authorLi, KC-
dc.contributor.authorSaif, L-
dc.contributor.authorBarrs, VR-
dc.contributor.authorWu, JT-
dc.contributor.authorSit, THC-
dc.contributor.authorPoon, LLM-
dc.contributor.authorPeiris, M-
dc.date.accessioned2021-01-11T13:57:12Z-
dc.date.available2021-01-11T13:57:12Z-
dc.date.issued2020-
dc.identifier.citationJournal of Clinical Microbiology, 2020, v. 59 n. 2, p. article no. e02504-20-
dc.identifier.issn0095-1137-
dc.identifier.urihttp://hdl.handle.net/10722/295231-
dc.description.abstractSurrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat, and hamster sera. With PRNT90 as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT90, except for cross-reactivity to SARS-CoV-1 in sVNT.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/-
dc.relation.ispartofJournal of Clinical Microbiology-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.-
dc.subjectSARS coronavirus 2-
dc.subjectCOVID-19-
dc.subjectserology-
dc.subjectneutralization-
dc.subjectseroepidemiology-
dc.titleEvaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat, and hamster sera-
dc.typeArticle-
dc.identifier.emailPerera, RAPM: mahenp@hku.hk-
dc.identifier.emailKo, R: koronald@hku.hk-
dc.identifier.emailYen, HL: hyen@hku.hk-
dc.identifier.emailLeung, K: ksmleung@hku.hk-
dc.identifier.emailChan, KH: ra98466@hku.hk-
dc.identifier.emailChan, KCK: karlcck@hku.hk-
dc.identifier.emailLi, KC: kc109698@hku.hk-
dc.identifier.emailWu, JT: joewu@hku.hk-
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hk-
dc.identifier.emailPeiris, M: malik@hkucc.hku.hk-
dc.identifier.authorityPerera, RAPM=rp02500-
dc.identifier.authorityYen, HL=rp00304-
dc.identifier.authorityLeung, K=rp02563-
dc.identifier.authorityWu, JT=rp00517-
dc.identifier.authorityPoon, LLM=rp00484-
dc.identifier.authorityPeiris, M=rp00410-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JCM.02504-20-
dc.identifier.pmid33139421-
dc.identifier.pmcidPMC8111130-
dc.identifier.scopuseid_2-s2.0-85099917560-
dc.identifier.hkuros320848-
dc.identifier.volume59-
dc.identifier.issue2-
dc.identifier.spagearticle no. e02504-
dc.identifier.epage20-
dc.identifier.isiWOS:000610235700037-
dc.publisher.placeUnited States-

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