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Article: Evaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat, and hamster sera

TitleEvaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat, and hamster sera
Authors
KeywordsSARS coronavirus 2
COVID-19
serology
neutralization
seroepidemiology
Issue Date2020
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/
Citation
Journal of Clinical Microbiology, 2020, v. 59 n. 2, p. article no. e02504-20 How to Cite?
AbstractSurrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat, and hamster sera. With PRNT90 as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT90, except for cross-reactivity to SARS-CoV-1 in sVNT.
Persistent Identifierhttp://hdl.handle.net/10722/295231
ISSN
2020 Impact Factor: 5.948
2020 SCImago Journal Rankings: 2.349
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPerera, RAPM-
dc.contributor.authorKo, R-
dc.contributor.authorTsang, OTY-
dc.contributor.authorHui, DSC-
dc.contributor.authorKwan, MYM-
dc.contributor.authorBrackman, CJ-
dc.contributor.authorTo, EMW-
dc.contributor.authorYen, HL-
dc.contributor.authorLeung, K-
dc.contributor.authorCheng, SMS-
dc.contributor.authorChan, KH-
dc.contributor.authorChan, KCK-
dc.contributor.authorLi, KC-
dc.contributor.authorSaif, L-
dc.contributor.authorBarrs, VR-
dc.contributor.authorWu, JT-
dc.contributor.authorSit, THC-
dc.contributor.authorPoon, LLM-
dc.contributor.authorPeiris, M-
dc.date.accessioned2021-01-11T13:57:12Z-
dc.date.available2021-01-11T13:57:12Z-
dc.date.issued2020-
dc.identifier.citationJournal of Clinical Microbiology, 2020, v. 59 n. 2, p. article no. e02504-20-
dc.identifier.issn0095-1137-
dc.identifier.urihttp://hdl.handle.net/10722/295231-
dc.description.abstractSurrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat, and hamster sera. With PRNT90 as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT90, except for cross-reactivity to SARS-CoV-1 in sVNT.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/-
dc.relation.ispartofJournal of Clinical Microbiology-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.-
dc.subjectSARS coronavirus 2-
dc.subjectCOVID-19-
dc.subjectserology-
dc.subjectneutralization-
dc.subjectseroepidemiology-
dc.titleEvaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat, and hamster sera-
dc.typeArticle-
dc.identifier.emailPerera, RAPM: mahenp@hku.hk-
dc.identifier.emailKo, R: koronald@hku.hk-
dc.identifier.emailYen, HL: hyen@hku.hk-
dc.identifier.emailLeung, K: ksmleung@hku.hk-
dc.identifier.emailChan, KH: ra98466@hku.hk-
dc.identifier.emailChan, KCK: karlcck@hku.hk-
dc.identifier.emailLi, KC: kc109698@hku.hk-
dc.identifier.emailWu, JT: joewu@hku.hk-
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hk-
dc.identifier.emailPeiris, M: malik@hkucc.hku.hk-
dc.identifier.authorityPerera, RAPM=rp02500-
dc.identifier.authorityYen, HL=rp00304-
dc.identifier.authorityLeung, K=rp02563-
dc.identifier.authorityWu, JT=rp00517-
dc.identifier.authorityPoon, LLM=rp00484-
dc.identifier.authorityPeiris, M=rp00410-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JCM.02504-20-
dc.identifier.pmid33139421-
dc.identifier.pmcidPMC8111130-
dc.identifier.scopuseid_2-s2.0-85099917560-
dc.identifier.hkuros320848-
dc.identifier.volume59-
dc.identifier.issue2-
dc.identifier.spagearticle no. e02504-
dc.identifier.epage20-
dc.identifier.isiWOS:000610235700037-
dc.publisher.placeUnited States-

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