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- Publisher Website: 10.1093/carcin/bgaa025
- Scopus: eid_2-s2.0-85092414662
- PMID: 32415843
- WOS: WOS:000593339000014
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Article: MicroRNA-135a-induced formation of CD133+ subpopulation with cancer stem cell properties in cervical cancer
Title | MicroRNA-135a-induced formation of CD133+ subpopulation with cancer stem cell properties in cervical cancer |
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Authors | |
Issue Date | 2020 |
Publisher | Oxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/ |
Citation | Carcinogenesis, 2020, v. 41 n. 11, p. 1592-1604 How to Cite? |
Abstract | Cancer stem cells (CSCs) play significant roles in tumor initiation. MicroRNA-135a (miR-135a) induced the formation of a CD133+ subpopulation from a human papillomavirus-immortalized cervical epithelial cell line. Compared with the CD133− cells, the CD133+ cells expressed higher levels of miR-135a and OCT4, exhibited significantly higher tumorsphere forming capacity and the time required for tumorsphere formation was shortened in the second generation. Serum induction suppressed the expression of CD133, OCT4 and miR-135a, but increased expression of involucrin in the miR-135a-induced CD133+ cells. The miR-135a-induced CD133+ cells were tumorigenic in a limiting dilution approach in vivo. The cells expressed significantly higher level of active β-catenin and OCT4 than the CD133− counterpart. Wnt3a enhanced the expression of OCT4 and CD133 in cervical cancer cells but failed to enhance CD133 transcription in normal cervical cells. Wnt3a stimulation also increased tumorsphere size and self-renewal of miR-135a-induced CD133+ subpopulation. Wnt/β-catenin inhibition suppressed tumorsphere formation while Wnt3a partially nullified the inhibitory effect. Taken together, miR-135a induced the formation of a subpopulation of cells with CSC properties both in vitro and in vivo and the Wnt/β-catenin signaling pathway is essential to maintain its tumorigenicity. |
Persistent Identifier | http://hdl.handle.net/10722/294015 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 1.074 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Leung, CON | - |
dc.contributor.author | Deng, W | - |
dc.contributor.author | Ye, TM | - |
dc.contributor.author | Ngan, HYS | - |
dc.contributor.author | Tsao, SW | - |
dc.contributor.author | Cheung, ANY | - |
dc.contributor.author | Niu, Z | - |
dc.contributor.author | Yuen, DCK | - |
dc.contributor.author | Pang, RTK | - |
dc.contributor.author | Yeung, WSB | - |
dc.date.accessioned | 2020-11-23T08:25:08Z | - |
dc.date.available | 2020-11-23T08:25:08Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Carcinogenesis, 2020, v. 41 n. 11, p. 1592-1604 | - |
dc.identifier.issn | 0143-3334 | - |
dc.identifier.uri | http://hdl.handle.net/10722/294015 | - |
dc.description.abstract | Cancer stem cells (CSCs) play significant roles in tumor initiation. MicroRNA-135a (miR-135a) induced the formation of a CD133+ subpopulation from a human papillomavirus-immortalized cervical epithelial cell line. Compared with the CD133− cells, the CD133+ cells expressed higher levels of miR-135a and OCT4, exhibited significantly higher tumorsphere forming capacity and the time required for tumorsphere formation was shortened in the second generation. Serum induction suppressed the expression of CD133, OCT4 and miR-135a, but increased expression of involucrin in the miR-135a-induced CD133+ cells. The miR-135a-induced CD133+ cells were tumorigenic in a limiting dilution approach in vivo. The cells expressed significantly higher level of active β-catenin and OCT4 than the CD133− counterpart. Wnt3a enhanced the expression of OCT4 and CD133 in cervical cancer cells but failed to enhance CD133 transcription in normal cervical cells. Wnt3a stimulation also increased tumorsphere size and self-renewal of miR-135a-induced CD133+ subpopulation. Wnt/β-catenin inhibition suppressed tumorsphere formation while Wnt3a partially nullified the inhibitory effect. Taken together, miR-135a induced the formation of a subpopulation of cells with CSC properties both in vitro and in vivo and the Wnt/β-catenin signaling pathway is essential to maintain its tumorigenicity. | - |
dc.language | eng | - |
dc.publisher | Oxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/ | - |
dc.relation.ispartof | Carcinogenesis | - |
dc.rights | Pre-print: Journal Title] ©: [year] [owner as specified on the article] Published by Oxford University Press [on behalf of xxxxxx]. All rights reserved. Pre-print (Once an article is published, preprint notice should be amended to): This is an electronic version of an article published in [include the complete citation information for the final version of the Article as published in the print edition of the Journal.] Post-print: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: xxxxxxx [insert URL that the author will receive upon publication here]. | - |
dc.title | MicroRNA-135a-induced formation of CD133+ subpopulation with cancer stem cell properties in cervical cancer | - |
dc.type | Article | - |
dc.identifier.email | Deng, W: wdeng@hku.hk | - |
dc.identifier.email | Ngan, HYS: hysngan@hkucc.hku.hk | - |
dc.identifier.email | Tsao, SW: gswtsao@hku.hk | - |
dc.identifier.email | Cheung, ANY: anycheun@hkucc.hku.hk | - |
dc.identifier.email | Yeung, WSB: wsbyeung@hku.hk | - |
dc.identifier.authority | Deng, W=rp01640 | - |
dc.identifier.authority | Ngan, HYS=rp00346 | - |
dc.identifier.authority | Tsao, SW=rp00399 | - |
dc.identifier.authority | Cheung, ANY=rp00542 | - |
dc.identifier.authority | Pang, RTK=rp01761 | - |
dc.identifier.authority | Yeung, WSB=rp00331 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1093/carcin/bgaa025 | - |
dc.identifier.pmid | 32415843 | - |
dc.identifier.scopus | eid_2-s2.0-85092414662 | - |
dc.identifier.hkuros | 319446 | - |
dc.identifier.hkuros | 316308 | - |
dc.identifier.volume | 41 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 1592 | - |
dc.identifier.epage | 1604 | - |
dc.identifier.isi | WOS:000593339000014 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0143-3334 | - |