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Article: β1 integrin regulates convergent extension in mouse notogenesis, ensures notochord integrity and the morphogenesis of vertebrae and intervertebral discs

Titleβ1 integrin regulates convergent extension in mouse notogenesis, ensures notochord integrity and the morphogenesis of vertebrae and intervertebral discs
Authors
Keywordsβ1 integrin
Notochord
Convergent extension
Intervertebral discs
Hemi-vertebrae
Planar cell polarity
Issue Date2020
PublisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
Citation
Development, 2020, v. 147 n. 22, article no. dev192724 How to Cite?
AbstractThe notochord drives longitudinal growth of the body axis by convergent extension, a highly conserved developmental process that depends on non-canonical Wnt/planar cell polarity (PCP) signaling. However, the role of cell-matrix interactions mediated by integrins in the development of the notochord is unclear. We developed transgenic Cre mice, in which the β1 integrin gene (Itgb1) is ablated at E8.0 in the notochord only or in the notochord and tail bud. These Itgb1 conditional mutants display misaligned, malformed vertebral bodies, hemi-vertebrae and truncated tails. From early somite stages, the notochord was interrupted and displaced in these mutants. Convergent extension of the notochord was impaired with defective cell movement. Treatment of E7.25 wild-type embryos with anti-β1 integrin blocking antibodies, to target node pit cells, disrupted asymmetric localization of VANGL2. Our study implicates pivotal roles of β1 integrin for the establishment of PCP and convergent extension of the developing notochord, its structural integrity and positioning, thereby ensuring development of the nucleus pulposus and the proper alignment of vertebral bodies and intervertebral discs. Failure of this control may contribute to human congenital spine malformations.
Persistent Identifierhttp://hdl.handle.net/10722/293491
ISSN
2020 Impact Factor: 6.868
2020 SCImago Journal Rankings: 3.754
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGuo, SS-
dc.contributor.authorAu, TYK-
dc.contributor.authorWynn, S-
dc.contributor.authorAszodi, A-
dc.contributor.authorChan, D-
dc.contributor.authorFässler, R-
dc.contributor.authorCheah, KSE-
dc.date.accessioned2020-11-23T08:17:33Z-
dc.date.available2020-11-23T08:17:33Z-
dc.date.issued2020-
dc.identifier.citationDevelopment, 2020, v. 147 n. 22, article no. dev192724-
dc.identifier.issn0950-1991-
dc.identifier.urihttp://hdl.handle.net/10722/293491-
dc.description.abstractThe notochord drives longitudinal growth of the body axis by convergent extension, a highly conserved developmental process that depends on non-canonical Wnt/planar cell polarity (PCP) signaling. However, the role of cell-matrix interactions mediated by integrins in the development of the notochord is unclear. We developed transgenic Cre mice, in which the β1 integrin gene (Itgb1) is ablated at E8.0 in the notochord only or in the notochord and tail bud. These Itgb1 conditional mutants display misaligned, malformed vertebral bodies, hemi-vertebrae and truncated tails. From early somite stages, the notochord was interrupted and displaced in these mutants. Convergent extension of the notochord was impaired with defective cell movement. Treatment of E7.25 wild-type embryos with anti-β1 integrin blocking antibodies, to target node pit cells, disrupted asymmetric localization of VANGL2. Our study implicates pivotal roles of β1 integrin for the establishment of PCP and convergent extension of the developing notochord, its structural integrity and positioning, thereby ensuring development of the nucleus pulposus and the proper alignment of vertebral bodies and intervertebral discs. Failure of this control may contribute to human congenital spine malformations.-
dc.languageeng-
dc.publisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org-
dc.relation.ispartofDevelopment-
dc.subjectβ1 integrin-
dc.subjectNotochord-
dc.subjectConvergent extension-
dc.subjectIntervertebral discs-
dc.subjectHemi-vertebrae-
dc.subjectPlanar cell polarity-
dc.titleβ1 integrin regulates convergent extension in mouse notogenesis, ensures notochord integrity and the morphogenesis of vertebrae and intervertebral discs-
dc.typeArticle-
dc.identifier.emailChan, D: chand@hku.hk-
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hk-
dc.identifier.authorityChan, D=rp00540-
dc.identifier.authorityCheah, KSE=rp00342-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1242/dev.192724-
dc.identifier.pmid33051257-
dc.identifier.scopuseid_2-s2.0-85096456827-
dc.identifier.hkuros318749-
dc.identifier.volume147-
dc.identifier.issue22-
dc.identifier.spagearticle no. dev192724-
dc.identifier.epagearticle no. dev192724-
dc.identifier.isiWOS:000596028000024-
dc.publisher.placeUnited Kingdom-

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