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Article: Flavonoids reduces lipopolysaccharide-induced release of inflammatory mediators in human bronchial epithelial cells: Structure-activity relationship

TitleFlavonoids reduces lipopolysaccharide-induced release of inflammatory mediators in human bronchial epithelial cells: Structure-activity relationship
Authors
KeywordsBronchial epithelial cells
Cyclic adenosine monophosphate
Flavonoids
Inflammation
Lipopolysaccharide
Issue Date2019
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal of Pharmacology, 2019, v. 865, p. article no. 172731 How to Cite?
AbstractFlavonoids are polyphenolic compounds that are widely present in food and Chinese medicine. The aim of the present study was to identify the flavonoids with anti-inflammatory effects in the airway; and to determine the role of anti-oxidant and cyclic adenosine monophosphate (cAMP) in the anti-inflammatory effect. Human bronchial epithelial BEAS-2B cells were exposed to bacterial endotoxin lipopolysaccharide (LPS) in the absence or presence of different flavonoids, which are categorized according to their chemical structures in seven subclasses [anthocyanidins, chalcones, flavanes, flavanones, flavones, flavonols, isoflavones]. Among the 17 flavonoids tested, only apigenin (flavones), luteolin (flavones), daidzein (isoflavones) and genistein (isoflavones) reduced LPS-induced release of inflammatory cytokines/chemokines interleukin (IL)-6, IL-8 and monocyte chemoattractant protein-1 in BEAS-2B cells. Quercetin caused further increase in LPS-induced IL-6 and IL-8 levels. It alone significantly increased nuclear factor-kappa B (NF-κB) p65 activity and the cellular oxidative stress marker malondialdehyde (MDA) level in BEAS-2B cells. By contrast, apigenin and genistein reduced LPS-induced increases in nuclear NF-κB activity and MDA level. Apigenin and genistein, but not quercetin, increased the cAMP level in BEAS-2B cells, and the cell-permeable cAMP analogue, 8-Br-cAMP, inhibited LPS-induced increase of IL-8 level. These findings suggest that the presence of C5-OH, C7-OH, C2=C3 and C4=O functional groups in the flavonoids is associated with greater anti-inflammatory effect, while that of C3-OH or glycosylation group at the A-ring greatly decreased the anti-inflammatory effect. The anti-inflammatory effect of these flavonoids may be related to their anti-oxidant properties, and partly to their ability in increasing cAMP level.
Persistent Identifierhttp://hdl.handle.net/10722/290933
ISSN
2021 Impact Factor: 5.195
2020 SCImago Journal Rankings: 1.046
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZHANG, P-
dc.contributor.authorMak, JCW-
dc.contributor.authorMan, RYK-
dc.contributor.authorLeung, SWS-
dc.date.accessioned2020-11-02T05:49:09Z-
dc.date.available2020-11-02T05:49:09Z-
dc.date.issued2019-
dc.identifier.citationEuropean Journal of Pharmacology, 2019, v. 865, p. article no. 172731-
dc.identifier.issn0014-2999-
dc.identifier.urihttp://hdl.handle.net/10722/290933-
dc.description.abstractFlavonoids are polyphenolic compounds that are widely present in food and Chinese medicine. The aim of the present study was to identify the flavonoids with anti-inflammatory effects in the airway; and to determine the role of anti-oxidant and cyclic adenosine monophosphate (cAMP) in the anti-inflammatory effect. Human bronchial epithelial BEAS-2B cells were exposed to bacterial endotoxin lipopolysaccharide (LPS) in the absence or presence of different flavonoids, which are categorized according to their chemical structures in seven subclasses [anthocyanidins, chalcones, flavanes, flavanones, flavones, flavonols, isoflavones]. Among the 17 flavonoids tested, only apigenin (flavones), luteolin (flavones), daidzein (isoflavones) and genistein (isoflavones) reduced LPS-induced release of inflammatory cytokines/chemokines interleukin (IL)-6, IL-8 and monocyte chemoattractant protein-1 in BEAS-2B cells. Quercetin caused further increase in LPS-induced IL-6 and IL-8 levels. It alone significantly increased nuclear factor-kappa B (NF-κB) p65 activity and the cellular oxidative stress marker malondialdehyde (MDA) level in BEAS-2B cells. By contrast, apigenin and genistein reduced LPS-induced increases in nuclear NF-κB activity and MDA level. Apigenin and genistein, but not quercetin, increased the cAMP level in BEAS-2B cells, and the cell-permeable cAMP analogue, 8-Br-cAMP, inhibited LPS-induced increase of IL-8 level. These findings suggest that the presence of C5-OH, C7-OH, C2=C3 and C4=O functional groups in the flavonoids is associated with greater anti-inflammatory effect, while that of C3-OH or glycosylation group at the A-ring greatly decreased the anti-inflammatory effect. The anti-inflammatory effect of these flavonoids may be related to their anti-oxidant properties, and partly to their ability in increasing cAMP level.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar-
dc.relation.ispartofEuropean Journal of Pharmacology-
dc.subjectBronchial epithelial cells-
dc.subjectCyclic adenosine monophosphate-
dc.subjectFlavonoids-
dc.subjectInflammation-
dc.subjectLipopolysaccharide-
dc.titleFlavonoids reduces lipopolysaccharide-induced release of inflammatory mediators in human bronchial epithelial cells: Structure-activity relationship-
dc.typeArticle-
dc.identifier.emailMak, JCW: judithmak@hku.hk-
dc.identifier.emailLeung, SWS: swsleung@hku.hk-
dc.identifier.authorityMak, JCW=rp00352-
dc.identifier.authorityMan, RYK=rp00236-
dc.identifier.authorityLeung, SWS=rp00235-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ejphar.2019.172731-
dc.identifier.pmid31610186-
dc.identifier.scopuseid_2-s2.0-85073223949-
dc.identifier.hkuros317945-
dc.identifier.volume865-
dc.identifier.spagearticle no. 172731-
dc.identifier.epagearticle no. 172731-
dc.identifier.isiWOS:000500178300016-
dc.publisher.placeNetherlands-
dc.identifier.issnl0014-2999-

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