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- Publisher Website: 10.1038/s41416-020-0922-7
- Scopus: eid_2-s2.0-85086028011
- PMID: 32507856
- WOS: WOS:000538706900004
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Article: CD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma
Title | CD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma |
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Authors | |
Keywords | animal experiment animal modelanimal tissue C-33 A cell line C-4-I cell line |
Issue Date | 2020 |
Publisher | Springer Nature, published in association with Cancer Research UK. The Journal's web site is located at http://www.nature.com/bjc |
Citation | British Journal of Cancer, 2020, v. 123 n. 5, p. 833-843 How to Cite? |
Abstract | Background:
CD109 was involved in the tumorigenesis and progression of various cancers via TGF-β1 signalling and STAT3 activation. As CD109 is strongly expressed in cervical squamous cell carcinoma, this study was conducted to investigate its functional characteristics in cervical cancer.
Methods:
CD109 expression was examined by immunohistochemistry (IHC) with cervical tissue microarray. The effects of CD109 expression were examined on migration, cell proliferation, spheroid formation and soft-agar colony-formation assay. Meanwhile, cervical cancer cell lines with high CD109 expression were chosen for the functional study using siRNA knockdown and CRISPR/Cas9 knockout.
Results:
IHC demonstrated an upregulation of CD109 in the cell membrane of cervical squamous cell carcinoma. CD109( + ) cells isolated by flow-cytometric sorting displayed enhanced migration, cell proliferation, sphere-forming and anchorage-independent cell growth ability. In contrast, silencing of CD109 expression could reverse the in vitro and in vivo tumorigenic and aggressive properties. Furthermore, CD109 induced EGFR-mediated STAT3 phosphorylation known to be responsible for cell migration, proliferation and maintenance of CSC phenotype.
Conclusion:
Abundant CD109( + ) populations in cervical cancer cells potentially contributed to carcinogenesis and aggressiveness, whereas silencing of CD109 expression could reverse those properties. CD109 mediates cervical tumorigenicity and aggressiveness via CD109/EGFR/STAT3 signalling. |
Persistent Identifier | http://hdl.handle.net/10722/289345 |
ISSN | 2023 Impact Factor: 6.4 2023 SCImago Journal Rankings: 3.000 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | MO, XT | - |
dc.contributor.author | Leung, THY | - |
dc.contributor.author | Tang, HWM | - |
dc.contributor.author | Siu, MKY | - |
dc.contributor.author | WAN, PKT | - |
dc.contributor.author | Chan, KKL | - |
dc.contributor.author | Cheung, ANY | - |
dc.contributor.author | Ngan, HYS | - |
dc.date.accessioned | 2020-10-22T08:11:19Z | - |
dc.date.available | 2020-10-22T08:11:19Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | British Journal of Cancer, 2020, v. 123 n. 5, p. 833-843 | - |
dc.identifier.issn | 0007-0920 | - |
dc.identifier.uri | http://hdl.handle.net/10722/289345 | - |
dc.description.abstract | Background: CD109 was involved in the tumorigenesis and progression of various cancers via TGF-β1 signalling and STAT3 activation. As CD109 is strongly expressed in cervical squamous cell carcinoma, this study was conducted to investigate its functional characteristics in cervical cancer. Methods: CD109 expression was examined by immunohistochemistry (IHC) with cervical tissue microarray. The effects of CD109 expression were examined on migration, cell proliferation, spheroid formation and soft-agar colony-formation assay. Meanwhile, cervical cancer cell lines with high CD109 expression were chosen for the functional study using siRNA knockdown and CRISPR/Cas9 knockout. Results: IHC demonstrated an upregulation of CD109 in the cell membrane of cervical squamous cell carcinoma. CD109( + ) cells isolated by flow-cytometric sorting displayed enhanced migration, cell proliferation, sphere-forming and anchorage-independent cell growth ability. In contrast, silencing of CD109 expression could reverse the in vitro and in vivo tumorigenic and aggressive properties. Furthermore, CD109 induced EGFR-mediated STAT3 phosphorylation known to be responsible for cell migration, proliferation and maintenance of CSC phenotype. Conclusion: Abundant CD109( + ) populations in cervical cancer cells potentially contributed to carcinogenesis and aggressiveness, whereas silencing of CD109 expression could reverse those properties. CD109 mediates cervical tumorigenicity and aggressiveness via CD109/EGFR/STAT3 signalling. | - |
dc.language | eng | - |
dc.publisher | Springer Nature, published in association with Cancer Research UK. The Journal's web site is located at http://www.nature.com/bjc | - |
dc.relation.ispartof | British Journal of Cancer | - |
dc.subject | animal experiment | - |
dc.subject | animal modelanimal tissue | - |
dc.subject | C-33 A cell line | - |
dc.subject | C-4-I cell line | - |
dc.title | CD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma | - |
dc.type | Article | - |
dc.identifier.email | Siu, MKY: mkysiu@hku.hk | - |
dc.identifier.email | Chan, KKL: kklchan@hkucc.hku.hk | - |
dc.identifier.email | Cheung, ANY: anycheun@hkucc.hku.hk | - |
dc.identifier.email | Ngan, HYS: hysngan@hkucc.hku.hk | - |
dc.identifier.authority | Siu, MKY=rp00275 | - |
dc.identifier.authority | Chan, KKL=rp00499 | - |
dc.identifier.authority | Cheung, ANY=rp00542 | - |
dc.identifier.authority | Ngan, HYS=rp00346 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1038/s41416-020-0922-7 | - |
dc.identifier.pmid | 32507856 | - |
dc.identifier.pmcid | PMC7463003 | - |
dc.identifier.scopus | eid_2-s2.0-85086028011 | - |
dc.identifier.hkuros | 316300 | - |
dc.identifier.volume | 123 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 833 | - |
dc.identifier.epage | 843 | - |
dc.identifier.isi | WOS:000538706900004 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0007-0920 | - |