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Article: CD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma

TitleCD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma
Authors
Keywordsanimal experiment
animal modelanimal tissue
C-33 A cell line
C-4-I cell line
Issue Date2020
PublisherSpringer Nature, published in association with Cancer Research UK. The Journal's web site is located at http://www.nature.com/bjc
Citation
British Journal of Cancer, 2020, v. 123 n. 5, p. 833-843 How to Cite?
AbstractBackground: CD109 was involved in the tumorigenesis and progression of various cancers via TGF-β1 signalling and STAT3 activation. As CD109 is strongly expressed in cervical squamous cell carcinoma, this study was conducted to investigate its functional characteristics in cervical cancer. Methods: CD109 expression was examined by immunohistochemistry (IHC) with cervical tissue microarray. The effects of CD109 expression were examined on migration, cell proliferation, spheroid formation and soft-agar colony-formation assay. Meanwhile, cervical cancer cell lines with high CD109 expression were chosen for the functional study using siRNA knockdown and CRISPR/Cas9 knockout. Results: IHC demonstrated an upregulation of CD109 in the cell membrane of cervical squamous cell carcinoma. CD109( + ) cells isolated by flow-cytometric sorting displayed enhanced migration, cell proliferation, sphere-forming and anchorage-independent cell growth ability. In contrast, silencing of CD109 expression could reverse the in vitro and in vivo tumorigenic and aggressive properties. Furthermore, CD109 induced EGFR-mediated STAT3 phosphorylation known to be responsible for cell migration, proliferation and maintenance of CSC phenotype. Conclusion: Abundant CD109( + ) populations in cervical cancer cells potentially contributed to carcinogenesis and aggressiveness, whereas silencing of CD109 expression could reverse those properties. CD109 mediates cervical tumorigenicity and aggressiveness via CD109/EGFR/STAT3 signalling.
Persistent Identifierhttp://hdl.handle.net/10722/289345
ISSN
2020 Impact Factor: 7.64
2020 SCImago Journal Rankings: 2.833
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMO, XT-
dc.contributor.authorLeung, THY-
dc.contributor.authorTang, HWM-
dc.contributor.authorSiu, MKY-
dc.contributor.authorWAN, PKT-
dc.contributor.authorChan, KKL-
dc.contributor.authorCheung, ANY-
dc.contributor.authorNgan, HYS-
dc.date.accessioned2020-10-22T08:11:19Z-
dc.date.available2020-10-22T08:11:19Z-
dc.date.issued2020-
dc.identifier.citationBritish Journal of Cancer, 2020, v. 123 n. 5, p. 833-843-
dc.identifier.issn0007-0920-
dc.identifier.urihttp://hdl.handle.net/10722/289345-
dc.description.abstractBackground: CD109 was involved in the tumorigenesis and progression of various cancers via TGF-β1 signalling and STAT3 activation. As CD109 is strongly expressed in cervical squamous cell carcinoma, this study was conducted to investigate its functional characteristics in cervical cancer. Methods: CD109 expression was examined by immunohistochemistry (IHC) with cervical tissue microarray. The effects of CD109 expression were examined on migration, cell proliferation, spheroid formation and soft-agar colony-formation assay. Meanwhile, cervical cancer cell lines with high CD109 expression were chosen for the functional study using siRNA knockdown and CRISPR/Cas9 knockout. Results: IHC demonstrated an upregulation of CD109 in the cell membrane of cervical squamous cell carcinoma. CD109( + ) cells isolated by flow-cytometric sorting displayed enhanced migration, cell proliferation, sphere-forming and anchorage-independent cell growth ability. In contrast, silencing of CD109 expression could reverse the in vitro and in vivo tumorigenic and aggressive properties. Furthermore, CD109 induced EGFR-mediated STAT3 phosphorylation known to be responsible for cell migration, proliferation and maintenance of CSC phenotype. Conclusion: Abundant CD109( + ) populations in cervical cancer cells potentially contributed to carcinogenesis and aggressiveness, whereas silencing of CD109 expression could reverse those properties. CD109 mediates cervical tumorigenicity and aggressiveness via CD109/EGFR/STAT3 signalling.-
dc.languageeng-
dc.publisherSpringer Nature, published in association with Cancer Research UK. The Journal's web site is located at http://www.nature.com/bjc-
dc.relation.ispartofBritish Journal of Cancer-
dc.subjectanimal experiment-
dc.subjectanimal modelanimal tissue-
dc.subjectC-33 A cell line-
dc.subjectC-4-I cell line-
dc.titleCD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma-
dc.typeArticle-
dc.identifier.emailSiu, MKY: mkysiu@hku.hk-
dc.identifier.emailChan, KKL: kklchan@hkucc.hku.hk-
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hk-
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hk-
dc.identifier.authoritySiu, MKY=rp00275-
dc.identifier.authorityChan, KKL=rp00499-
dc.identifier.authorityCheung, ANY=rp00542-
dc.identifier.authorityNgan, HYS=rp00346-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1038/s41416-020-0922-7-
dc.identifier.pmid32507856-
dc.identifier.pmcidPMC7463003-
dc.identifier.scopuseid_2-s2.0-85086028011-
dc.identifier.hkuros316300-
dc.identifier.volume123-
dc.identifier.issue5-
dc.identifier.spage833-
dc.identifier.epage843-
dc.identifier.isiWOS:000538706900004-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0007-0920-

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