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Article: Differential metabolic and inflammatory responses to intermittent hypoxia in substrains of lean and obese C57BL/6 mice

TitleDifferential metabolic and inflammatory responses to intermittent hypoxia in substrains of lean and obese C57BL/6 mice
Authors
KeywordsAdipose tissue
Genetic background
Intermittent hypoxia (IH)
Obesity
Obstructive sleep apnea (OSA)
Issue Date2019
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2019, v. 238, article no. 116959 How to Cite?
AbstractAims: This study was to investigate the degree of susceptibility to intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), between the two mice inbred lines C57BL/6N (6N) and C57BL/6J (6J). Materials and methods: Four-week old male mice of 6N and 6J substrains (n = 8) were randomized to standard diet (SD) group or high fat (HF) diet group. At the age of 13-week, all two groups of mice were subjected to either air or IH (IH30; thirty hypoxic events per hour) for one week. Key findings: All mice fed with HF diet exhibited obesity with more body weight and fat mass (percentage to body weight) gain. IH reduced serum LDL, HDL and total cholesterol levels in lean 6J mice. In obese mice, IH lowered obesity-induced serum total cholesterol level in 6J substrain but raised further in 6N substrain. Furthermore, IH caused elevation of serum FFA and MDA levels, and pro-inflammatory cytokines MCP-1 and IL-6 levels in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) of lean 6J but not lean 6N mice. There was reduced number of adipocytes and elevation of macrophages in SAT and VAT of HF-induced obese mice of both substrains. IH led to increased number of adipocytes and macrophages in SAT of lean 6J mice. Significance: The genetic difference between 6N and 6J mice may have direct impact on metabolic and inflammatory responses after IH. Therefore, attention must be given for the selection of C57BL mice substrains in the experimental IH-exposed mouse model.
Persistent Identifierhttp://hdl.handle.net/10722/286237
ISSN
2023 Impact Factor: 5.2
2023 SCImago Journal Rankings: 1.257
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGe, MQ-
dc.contributor.authorYeung, SC-
dc.contributor.authorMak, JCW-
dc.contributor.authorIp, MSM-
dc.date.accessioned2020-08-31T07:01:06Z-
dc.date.available2020-08-31T07:01:06Z-
dc.date.issued2019-
dc.identifier.citationLife Sciences, 2019, v. 238, article no. 116959-
dc.identifier.issn0024-3205-
dc.identifier.urihttp://hdl.handle.net/10722/286237-
dc.description.abstractAims: This study was to investigate the degree of susceptibility to intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), between the two mice inbred lines C57BL/6N (6N) and C57BL/6J (6J). Materials and methods: Four-week old male mice of 6N and 6J substrains (n = 8) were randomized to standard diet (SD) group or high fat (HF) diet group. At the age of 13-week, all two groups of mice were subjected to either air or IH (IH30; thirty hypoxic events per hour) for one week. Key findings: All mice fed with HF diet exhibited obesity with more body weight and fat mass (percentage to body weight) gain. IH reduced serum LDL, HDL and total cholesterol levels in lean 6J mice. In obese mice, IH lowered obesity-induced serum total cholesterol level in 6J substrain but raised further in 6N substrain. Furthermore, IH caused elevation of serum FFA and MDA levels, and pro-inflammatory cytokines MCP-1 and IL-6 levels in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) of lean 6J but not lean 6N mice. There was reduced number of adipocytes and elevation of macrophages in SAT and VAT of HF-induced obese mice of both substrains. IH led to increased number of adipocytes and macrophages in SAT of lean 6J mice. Significance: The genetic difference between 6N and 6J mice may have direct impact on metabolic and inflammatory responses after IH. Therefore, attention must be given for the selection of C57BL mice substrains in the experimental IH-exposed mouse model.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie-
dc.relation.ispartofLife Sciences-
dc.subjectAdipose tissue-
dc.subjectGenetic background-
dc.subjectIntermittent hypoxia (IH)-
dc.subjectObesity-
dc.subjectObstructive sleep apnea (OSA)-
dc.titleDifferential metabolic and inflammatory responses to intermittent hypoxia in substrains of lean and obese C57BL/6 mice-
dc.typeArticle-
dc.identifier.emailYeung, SC: flag@hkucc.hku.hk-
dc.identifier.emailMak, JCW: judithmak@hku.hk-
dc.identifier.emailIp, MSM: msmip@hku.hk-
dc.identifier.authorityMak, JCW=rp00352-
dc.identifier.authorityIp, MSM=rp00347-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.lfs.2019.116959-
dc.identifier.pmid31628916-
dc.identifier.scopuseid_2-s2.0-85074393697-
dc.identifier.hkuros313198-
dc.identifier.volume238-
dc.identifier.spagearticle no. 116959-
dc.identifier.epagearticle no. 116959-
dc.identifier.isiWOS:000498852700014-
dc.publisher.placeUnited States-
dc.identifier.issnl0024-3205-

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