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Article: Multiphoton photochemical crosslinking-based fabrication of protein micropatterns with controllable mechanical properties for single cell traction force measurements

TitleMultiphoton photochemical crosslinking-based fabrication of protein micropatterns with controllable mechanical properties for single cell traction force measurements
Authors
Keywordsanimal
atomic force microscopy
biomechanics
bovine
cell line
Issue Date2016
PublisherNature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2016, v. 6, p. article no. 20063 How to Cite?
AbstractEngineering 3D microstructures with predetermined properties is critical for stem cell niche studies. We have developed a multiphoton femtosecond laser-based 3D printing platform, which generates complex protein microstructures in minutes. Here, we used the platform to test a series of fabrication and reagent parameters in precisely controlling the mechanical properties of protein micropillars. Atomic force microscopy was utilized to measure the reduced elastic modulus of the micropillars and transmission electron microscopy was used to visualize the porosity of the structures. The reduced elastic modulus of the micropillars associated positively and linearly with the scanning power. On the other hand, the porosity and pore size of the micropillars associated inversely and linearly with the scanning power and reagent concentrations. While keeping the elastic modulus constant, the stiffness of the micropillars was controlled by varying their height. Subsequently, the single cell traction forces of rabbit chondrocytes, human dermal fibroblasts, human mesenchymal stem cells and bovine nucleus pulposus cells (bNPCs) were successfully measured by culturing the cells on micropillar arrays of different stiffness. Our results showed that the traction forces of all groups showed positive relationship with stiffness and that the chondrocytes and bNPCs generated the highest and lowest traction forces, respectively.
Persistent Identifierhttp://hdl.handle.net/10722/284087
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 0.900
PubMed Central ID
ISI Accession Number ID
Grants

 

DC FieldValueLanguage
dc.contributor.authorTONG, MH-
dc.contributor.authorHUANG, N-
dc.contributor.authorZHANG, W-
dc.contributor.authorZHOU, ZL-
dc.contributor.authorNgan, AHW-
dc.contributor.authorDu, Y-
dc.contributor.authorChan, BP-
dc.date.accessioned2020-07-20T05:55:59Z-
dc.date.available2020-07-20T05:55:59Z-
dc.date.issued2016-
dc.identifier.citationScientific Reports, 2016, v. 6, p. article no. 20063-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/284087-
dc.description.abstractEngineering 3D microstructures with predetermined properties is critical for stem cell niche studies. We have developed a multiphoton femtosecond laser-based 3D printing platform, which generates complex protein microstructures in minutes. Here, we used the platform to test a series of fabrication and reagent parameters in precisely controlling the mechanical properties of protein micropillars. Atomic force microscopy was utilized to measure the reduced elastic modulus of the micropillars and transmission electron microscopy was used to visualize the porosity of the structures. The reduced elastic modulus of the micropillars associated positively and linearly with the scanning power. On the other hand, the porosity and pore size of the micropillars associated inversely and linearly with the scanning power and reagent concentrations. While keeping the elastic modulus constant, the stiffness of the micropillars was controlled by varying their height. Subsequently, the single cell traction forces of rabbit chondrocytes, human dermal fibroblasts, human mesenchymal stem cells and bovine nucleus pulposus cells (bNPCs) were successfully measured by culturing the cells on micropillar arrays of different stiffness. Our results showed that the traction forces of all groups showed positive relationship with stiffness and that the chondrocytes and bNPCs generated the highest and lowest traction forces, respectively.-
dc.languageeng-
dc.publisherNature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectanimal-
dc.subjectatomic force microscopy-
dc.subjectbiomechanics-
dc.subjectbovine-
dc.subjectcell line-
dc.titleMultiphoton photochemical crosslinking-based fabrication of protein micropatterns with controllable mechanical properties for single cell traction force measurements-
dc.typeArticle-
dc.identifier.emailNgan, AHW: hwngan@hku.hk-
dc.identifier.emailChan, BP: bpchan@hku.hk-
dc.identifier.authorityNgan, AHW=rp00225-
dc.identifier.authorityChan, BP=rp00087-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep20063-
dc.identifier.pmid26817674-
dc.identifier.pmcidPMC4730236-
dc.identifier.scopuseid_2-s2.0-84956536404-
dc.identifier.hkuros311454-
dc.identifier.volume6-
dc.identifier.spagearticle no. 20063-
dc.identifier.epagearticle no. 20063-
dc.identifier.isiWOS:000368779500002-
dc.publisher.placeUnited Kingdom-
dc.relation.projectRationalizing scaffold design with optimal cell niche for mesenchymal stem cell-based therapy in disc degeneration-
dc.identifier.issnl2045-2322-

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