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Article: In Vitro Salivary Protein Adsorption Profile on Titanium and Ceramic Surfaces and the Corresponding Putative Immunological Implications

TitleIn Vitro Salivary Protein Adsorption Profile on Titanium and Ceramic Surfaces and the Corresponding Putative Immunological Implications
Authors
Keywordsceramic
dental implants
immunology
salivary proteins and peptides
surface properties
Issue Date2020
PublisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms
Citation
International Journal of Molecular Sciences, 2020, v. 21 n. 9, p. article no. 3083 How to Cite?
AbstractImmune responses triggered by implant abutment surfaces contributed by surface-adsorbed proteins are critical in clinical implant integration. How material surface-adsorbed proteins relate to host immune responses remain unclear. This study aimed to profile and address the immunological roles of surface-adsorbed salivary proteins on conventional implant abutment materials. Standardized polished bocks (5 × 5 × 1 mm3) were prepared from titanium and feldspathic ceramic. Salivary acquired pellicle formed in vitro was examined by liquid chromatography-tandem mass spectrometry and gene ontology (GO) analysis to identify and characterize the adsorbed proteins. Out of 759 proteins identified from pooled saliva samples, 396 were found to be attached to the two materials tested—369 on titanium and 298 on ceramic, with 281 common to both. GO annotation of immune processes was undertaken to form a protein–protein interaction network, and 14 hub proteins (≥6 interaction partners) (coding genes: B2M, C3, CLU, DEFA1, HSP90AA1, HSP90AB1, LTF, PIGR, PSMA2, RAC1, RAP1A, S100A8, S100A9, and SLP1) were identified as the key proteins connecting multiple (6–9) immune processes. The results offered putative immunological prospects of implant abutment material surface-adsorbed salivary proteins, which could potentially underpin the dynamic nature of implant–mucosal/implant–microbial interactions.
Persistent Identifierhttp://hdl.handle.net/10722/282501
ISSN
2023 Impact Factor: 4.9
2023 SCImago Journal Rankings: 1.179
ISI Accession Number ID
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DC FieldValueLanguage
dc.contributor.authorWei, C-X-
dc.contributor.authorBurrow, MF-
dc.contributor.authorBotelho, MG-
dc.contributor.authorLam, H-
dc.contributor.authorLeung, WK-
dc.date.accessioned2020-05-15T05:28:56Z-
dc.date.available2020-05-15T05:28:56Z-
dc.date.issued2020-
dc.identifier.citationInternational Journal of Molecular Sciences, 2020, v. 21 n. 9, p. article no. 3083-
dc.identifier.issn1661-6596-
dc.identifier.urihttp://hdl.handle.net/10722/282501-
dc.description.abstractImmune responses triggered by implant abutment surfaces contributed by surface-adsorbed proteins are critical in clinical implant integration. How material surface-adsorbed proteins relate to host immune responses remain unclear. This study aimed to profile and address the immunological roles of surface-adsorbed salivary proteins on conventional implant abutment materials. Standardized polished bocks (5 × 5 × 1 mm3) were prepared from titanium and feldspathic ceramic. Salivary acquired pellicle formed in vitro was examined by liquid chromatography-tandem mass spectrometry and gene ontology (GO) analysis to identify and characterize the adsorbed proteins. Out of 759 proteins identified from pooled saliva samples, 396 were found to be attached to the two materials tested—369 on titanium and 298 on ceramic, with 281 common to both. GO annotation of immune processes was undertaken to form a protein–protein interaction network, and 14 hub proteins (≥6 interaction partners) (coding genes: B2M, C3, CLU, DEFA1, HSP90AA1, HSP90AB1, LTF, PIGR, PSMA2, RAC1, RAP1A, S100A8, S100A9, and SLP1) were identified as the key proteins connecting multiple (6–9) immune processes. The results offered putative immunological prospects of implant abutment material surface-adsorbed salivary proteins, which could potentially underpin the dynamic nature of implant–mucosal/implant–microbial interactions.-
dc.languageeng-
dc.publisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms-
dc.relation.ispartofInternational Journal of Molecular Sciences-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectceramic-
dc.subjectdental implants-
dc.subjectimmunology-
dc.subjectsalivary proteins and peptides-
dc.subjectsurface properties-
dc.titleIn Vitro Salivary Protein Adsorption Profile on Titanium and Ceramic Surfaces and the Corresponding Putative Immunological Implications-
dc.typeArticle-
dc.identifier.emailBurrow, MF: mfburr58@hku.hk-
dc.identifier.emailBotelho, MG: botelho@hkucc.hku.hk-
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hk-
dc.identifier.authorityBurrow, MF=rp01306-
dc.identifier.authorityBotelho, MG=rp00033-
dc.identifier.authorityLeung, WK=rp00019-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/ijms21093083-
dc.identifier.pmid32349305-
dc.identifier.scopuseid_2-s2.0-85083960637-
dc.identifier.hkuros309951-
dc.identifier.volume21-
dc.identifier.issue9-
dc.identifier.spagearticle no. 3083-
dc.identifier.epagearticle no. 3083-
dc.identifier.isiWOS:000535581700054-
dc.publisher.placeSwitzerland-
dc.relation.projectDental and restorative materials acquired pellicle proteomics and microbiomics: A key battle front to defense against recurrent oral disease.-
dc.identifier.issnl1422-0067-

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