File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Effects of Lactic Acid Bacteria-Fermented Soymilk on Isoflavone Metabolites and Short-Chain Fatty Acids Excretion and Their Modulating Effects on Gut Microbiota

TitleEffects of Lactic Acid Bacteria-Fermented Soymilk on Isoflavone Metabolites and Short-Chain Fatty Acids Excretion and Their Modulating Effects on Gut Microbiota
Authors
Keywordsbioavailability
fecal enzyme
gut microbiota
L. rhamnosus
soy isoflavone
Issue Date2019
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147
Citation
Journal of Food Science, 2019, v. 84 n. 7, p. 1854-1863 How to Cite?
AbstractLactobacillus rhamnosus strain ASCC 1520 with high soy isoflavone transformation ability was used to ferment soymilk and added to the diet of mice. The impact of L. rhamnosus fermentation on soy isoflavone metabolites and intestinal bacterial community, in conjunction with fecal enzyme activity and short‐chain fatty acids (SCFA) excretion was evaluated. Antibiotics intervention resulted in a decrease in fecal enzyme activities and SCFA. Although long‐term intake of soymilk or L. rhamnosus‐fermented soymilk did not affect the fecal β‐glucuronidase and β‐galactosidase activities, it improved the β‐glucosidase activity when antibiotics were concomitantly administered. Soymilk or fermented soymilk administration increased the isoflavone metabolites (O‐DMA and equol) excreted in urine. Antibiotics decreased the daidzein excretion and its metabolites but showed little effect on glycitein and genistein excretion. Principal coordinates analysis (PCoA) of the 16s rRNA gene sequencing data found a remarkable shift in gut microbiota after soymilk administration and antibiotics treatment. Matastats test of the relative abundance of bacterial taxa revealed Odoribacter (Bacteroidales family), Lactobacillus (Lactobacillales order), and Alistipes (Rikenellaceae family) were enriched in soymilk while bacterial taxa from Bacteroides and Lactobacillus were enriched in L. rhamnosus‐fermented soymilk. Furthermore, there was less decrease in bacterial taxa with fermented soymilk group even when antibiotics were concomitantly administered. Overall, this study revealed that the gut microbiota of a healthy host is enough for the whole isoflavone metabolism under normal conditions. Feeding mice with L. rhamnosus‐fermented soymilk improved fecal enzyme activity and kept the balance of the gut mirobiota when antibiotics were used. Practical Application: Feeding mice with L. rhamnosus‐fermented soymilk improved fecal enzyme activity and kept the balance of the gut mirobiota when antibiotics were used.
Persistent Identifierhttp://hdl.handle.net/10722/271359
ISSN
2023 Impact Factor: 3.2
2023 SCImago Journal Rankings: 0.783
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDai, S-
dc.contributor.authorPan, M-
dc.contributor.authorEl-Nezamy, HS-
dc.contributor.authorWan, JMF-
dc.contributor.authorWang, MF-
dc.contributor.authorHabimana, O-
dc.contributor.authorLee, JCY-
dc.contributor.authorLouie, JCY-
dc.contributor.authorShah, NP-
dc.date.accessioned2019-06-24T01:08:20Z-
dc.date.available2019-06-24T01:08:20Z-
dc.date.issued2019-
dc.identifier.citationJournal of Food Science, 2019, v. 84 n. 7, p. 1854-1863-
dc.identifier.issn0022-1147-
dc.identifier.urihttp://hdl.handle.net/10722/271359-
dc.description.abstractLactobacillus rhamnosus strain ASCC 1520 with high soy isoflavone transformation ability was used to ferment soymilk and added to the diet of mice. The impact of L. rhamnosus fermentation on soy isoflavone metabolites and intestinal bacterial community, in conjunction with fecal enzyme activity and short‐chain fatty acids (SCFA) excretion was evaluated. Antibiotics intervention resulted in a decrease in fecal enzyme activities and SCFA. Although long‐term intake of soymilk or L. rhamnosus‐fermented soymilk did not affect the fecal β‐glucuronidase and β‐galactosidase activities, it improved the β‐glucosidase activity when antibiotics were concomitantly administered. Soymilk or fermented soymilk administration increased the isoflavone metabolites (O‐DMA and equol) excreted in urine. Antibiotics decreased the daidzein excretion and its metabolites but showed little effect on glycitein and genistein excretion. Principal coordinates analysis (PCoA) of the 16s rRNA gene sequencing data found a remarkable shift in gut microbiota after soymilk administration and antibiotics treatment. Matastats test of the relative abundance of bacterial taxa revealed Odoribacter (Bacteroidales family), Lactobacillus (Lactobacillales order), and Alistipes (Rikenellaceae family) were enriched in soymilk while bacterial taxa from Bacteroides and Lactobacillus were enriched in L. rhamnosus‐fermented soymilk. Furthermore, there was less decrease in bacterial taxa with fermented soymilk group even when antibiotics were concomitantly administered. Overall, this study revealed that the gut microbiota of a healthy host is enough for the whole isoflavone metabolism under normal conditions. Feeding mice with L. rhamnosus‐fermented soymilk improved fecal enzyme activity and kept the balance of the gut mirobiota when antibiotics were used. Practical Application: Feeding mice with L. rhamnosus‐fermented soymilk improved fecal enzyme activity and kept the balance of the gut mirobiota when antibiotics were used.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147-
dc.relation.ispartofJournal of Food Science-
dc.rightsThis is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectbioavailability-
dc.subjectfecal enzyme-
dc.subjectgut microbiota-
dc.subjectL. rhamnosus-
dc.subjectsoy isoflavone-
dc.titleEffects of Lactic Acid Bacteria-Fermented Soymilk on Isoflavone Metabolites and Short-Chain Fatty Acids Excretion and Their Modulating Effects on Gut Microbiota-
dc.typeArticle-
dc.identifier.emailEl-Nezamy, HS: elnezami@hkucc.hku.hk-
dc.identifier.emailWan, JMF: jmfwan@hku.hk-
dc.identifier.emailWang, MF: mfwang@hku.hk-
dc.identifier.emailHabimana, O: ohabim@hku.hk-
dc.identifier.emailLee, JCY: jettylee@hku.hk-
dc.identifier.emailLouie, JCY: jimmyl@hku.hk-
dc.identifier.emailShah, NP: npshah@hku.hk-
dc.identifier.authorityEl-Nezamy, HS=rp00694-
dc.identifier.authorityWan, JMF=rp00798-
dc.identifier.authorityWang, MF=rp00800-
dc.identifier.authorityHabimana, O=rp02169-
dc.identifier.authorityLee, JCY=rp01511-
dc.identifier.authorityLouie, JCY=rp02118-
dc.identifier.authorityShah, NP=rp01571-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/1750-3841.14661-
dc.identifier.scopuseid_2-s2.0-85067839030-
dc.identifier.hkuros298199-
dc.identifier.volume84-
dc.identifier.issue7-
dc.identifier.spage1854-
dc.identifier.epage1863-
dc.identifier.isiWOS:000482259600011-
dc.publisher.placeUnited States-
dc.identifier.issnl0022-1147-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats