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Conference Paper: EBV-associated Histone Modifications in the Regulation of DNA Damage Repair Members in Nasopharyngeal Carcinoma.
Title | EBV-associated Histone Modifications in the Regulation of DNA Damage Repair Members in Nasopharyngeal Carcinoma. |
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Authors | |
Keywords | EBV Histone modification NPC |
Issue Date | 2017 |
Publisher | Blackwell Publishing Japan. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CAS |
Citation | The 76th Annual Meeting of the Japanese Cancer Association, Yokohama, Japan, 28-30 September 2017. Abstracts in Cancer Science, 2017, v. 109 n. Suppl. 1, p. 184 How to Cite? |
Abstract | Infection of an oncovirus, Epstein - Barr virus (EBV), is associated with certain cancers including nasopharygeal carcinoma (NPC). However, the possible roles of EBV in NPC development are still poorly understood. In this study, we aimed to elucidate the roles of EBV in the regulation of DNA damage repair members via histone modifications H3K4me3. Two pairs of EBV-positive and EBV-negative nasopharygeal epithelial (NPE) cell lines were used for chromatin immunoprecipitation sequencing (ChIP-Seq) to identify EBV-regulated genes in the host cells. Totally, 18 DNA damage repair signalling members showed significant losses of H3K4me3 in EBV-infected NPE cells. Among all the candidate genes, 7 base excision repair (BER) members, APEX, POLB , POLD1 , PCNA, TDG, OGG1, and NEIL3 , and a mismatch repair (MMR) member, MLH1 , showed significant downregulation in the EBV-infected cells. The clinical significance was further confirmed by detection of significant down-regulation of the BER members and MLH1 in NPC paired biopsies. Our results suggested EBV infection induces loss of H3K4me3 in the BER members and MLH1 , resulting in downregulation in the EBV-infected cells and NPC tumor biopsies. |
Description | Poster Sessions (P3-2): EBV - no. P-1037 |
Persistent Identifier | http://hdl.handle.net/10722/258496 |
ISSN | 2023 Impact Factor: 4.5 2023 SCImago Journal Rankings: 1.625 |
DC Field | Value | Language |
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dc.contributor.author | Leong, ML | - |
dc.contributor.author | Cheung, AKL | - |
dc.contributor.author | Dai, W | - |
dc.contributor.author | Tsao, GSW | - |
dc.contributor.author | Lung, ML | - |
dc.date.accessioned | 2018-08-22T01:39:27Z | - |
dc.date.available | 2018-08-22T01:39:27Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | The 76th Annual Meeting of the Japanese Cancer Association, Yokohama, Japan, 28-30 September 2017. Abstracts in Cancer Science, 2017, v. 109 n. Suppl. 1, p. 184 | - |
dc.identifier.issn | 1347-9032 | - |
dc.identifier.uri | http://hdl.handle.net/10722/258496 | - |
dc.description | Poster Sessions (P3-2): EBV - no. P-1037 | - |
dc.description.abstract | Infection of an oncovirus, Epstein - Barr virus (EBV), is associated with certain cancers including nasopharygeal carcinoma (NPC). However, the possible roles of EBV in NPC development are still poorly understood. In this study, we aimed to elucidate the roles of EBV in the regulation of DNA damage repair members via histone modifications H3K4me3. Two pairs of EBV-positive and EBV-negative nasopharygeal epithelial (NPE) cell lines were used for chromatin immunoprecipitation sequencing (ChIP-Seq) to identify EBV-regulated genes in the host cells. Totally, 18 DNA damage repair signalling members showed significant losses of H3K4me3 in EBV-infected NPE cells. Among all the candidate genes, 7 base excision repair (BER) members, APEX, POLB , POLD1 , PCNA, TDG, OGG1, and NEIL3 , and a mismatch repair (MMR) member, MLH1 , showed significant downregulation in the EBV-infected cells. The clinical significance was further confirmed by detection of significant down-regulation of the BER members and MLH1 in NPC paired biopsies. Our results suggested EBV infection induces loss of H3K4me3 in the BER members and MLH1 , resulting in downregulation in the EBV-infected cells and NPC tumor biopsies. | - |
dc.language | eng | - |
dc.publisher | Blackwell Publishing Japan. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CAS | - |
dc.relation.ispartof | Cancer Science | - |
dc.relation.ispartof | The 76th Annual Meeting of the Japanese Cancer Association | - |
dc.rights | The definitive version is available at www.blackwell-synergy.com | - |
dc.subject | EBV | - |
dc.subject | Histone modification | - |
dc.subject | NPC | - |
dc.title | EBV-associated Histone Modifications in the Regulation of DNA Damage Repair Members in Nasopharyngeal Carcinoma. | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Cheung, AKL: arthurhk@hku.hk | - |
dc.identifier.email | Dai, W: weidai2@hku.hk | - |
dc.identifier.email | Tsao, GSW: gswtsao@hku.hk | - |
dc.identifier.email | Lung, ML: mlilung@hku.hk | - |
dc.identifier.authority | Cheung, AKL=rp01769 | - |
dc.identifier.authority | Dai, W=rp02146 | - |
dc.identifier.authority | Tsao, GSW=rp00399 | - |
dc.identifier.authority | Lung, ML=rp00300 | - |
dc.identifier.hkuros | 286588 | - |
dc.identifier.volume | 109 | - |
dc.identifier.issue | Suppl. 1 | - |
dc.identifier.spage | 184 | - |
dc.identifier.epage | 184 | - |
dc.publisher.place | Japan | - |
dc.identifier.issnl | 1347-9032 | - |