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- Publisher Website: 10.1385/BTER:86:2:123
- Scopus: eid_2-s2.0-0036240909
- PMID: 12008975
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Article: Immunoregulatory and antioxidant performance of α-tocopherol and selenium on human lymphocytes
Title | Immunoregulatory and antioxidant performance of α-tocopherol and selenium on human lymphocytes |
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Authors | |
Keywords | Cell-mediated immunity Flow cytometer Glutathione Human lymphocyte proliferation Hydrogen peroxide Selenium Vitamin E |
Issue Date | 2002 |
Citation | Biological Trace Element Research, 2002, v. 86 n. 2, p. 123-136 How to Cite? |
Abstract | The role of α-tocopherol (α-toco) and selenium (Se) on human lymphocyte oxidative stress and T-cells proliferation were studied by flow cytometry. We measured the hydrogen peroxide and glutathione levels in cultured human T-lymphocytes and the proliferation of their subsets: T-helper/inducer, T-suppressor/cytotoxic, and natural killer and interleukin-2 receptors upon stimulation by the mitogens phytohemaglutinin (PHA) and lipopolysaccharide (LPS). The results indicate that early stimulation by mitogens is affected by the glutathione and hydrogen peroxide status of the T-lymphocytes. The addition of 100 μM or 500 μM α-toco or 0.5 μM Se alone shows weak antioxidant and immunostimulant properties. When combined, an enhanced antioxidant and immunoregulatory effect was observed. The present findings indicate that α-toco and Se have interactive effects as oxygen radical scavengers, thus promoting human lymphocyte response to antigens. This suggests that micronutrient status is an important factor in considering when interpreting the results of in vitro assays of lymphocyte function. |
Persistent Identifier | http://hdl.handle.net/10722/178764 |
ISSN | 2023 Impact Factor: 3.4 2023 SCImago Journal Rankings: 0.764 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, CYJ | en_US |
dc.contributor.author | Wan, JMF | en_US |
dc.date.accessioned | 2012-12-19T09:49:36Z | - |
dc.date.available | 2012-12-19T09:49:36Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Biological Trace Element Research, 2002, v. 86 n. 2, p. 123-136 | en_US |
dc.identifier.issn | 0163-4984 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178764 | - |
dc.description.abstract | The role of α-tocopherol (α-toco) and selenium (Se) on human lymphocyte oxidative stress and T-cells proliferation were studied by flow cytometry. We measured the hydrogen peroxide and glutathione levels in cultured human T-lymphocytes and the proliferation of their subsets: T-helper/inducer, T-suppressor/cytotoxic, and natural killer and interleukin-2 receptors upon stimulation by the mitogens phytohemaglutinin (PHA) and lipopolysaccharide (LPS). The results indicate that early stimulation by mitogens is affected by the glutathione and hydrogen peroxide status of the T-lymphocytes. The addition of 100 μM or 500 μM α-toco or 0.5 μM Se alone shows weak antioxidant and immunostimulant properties. When combined, an enhanced antioxidant and immunoregulatory effect was observed. The present findings indicate that α-toco and Se have interactive effects as oxygen radical scavengers, thus promoting human lymphocyte response to antigens. This suggests that micronutrient status is an important factor in considering when interpreting the results of in vitro assays of lymphocyte function. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Biological Trace Element Research | en_US |
dc.subject | Cell-mediated immunity | - |
dc.subject | Flow cytometer | - |
dc.subject | Glutathione | - |
dc.subject | Human lymphocyte proliferation | - |
dc.subject | Hydrogen peroxide | - |
dc.subject | Selenium | - |
dc.subject | Vitamin E | - |
dc.subject.mesh | Antigens, Cd4 - Drug Effects | en_US |
dc.subject.mesh | Antigens, Cd56 - Drug Effects | en_US |
dc.subject.mesh | Antigens, Cd8 - Drug Effects | en_US |
dc.subject.mesh | Antioxidants - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Cell Differentiation - Drug Effects | en_US |
dc.subject.mesh | Cell Division - Drug Effects | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Drug Synergism | en_US |
dc.subject.mesh | Glutathione - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Hydrogen Peroxide - Metabolism | en_US |
dc.subject.mesh | Lipopolysaccharides - Toxicity | en_US |
dc.subject.mesh | Lymphocyte Subsets - Drug Effects | en_US |
dc.subject.mesh | Lymphocytes - Drug Effects - Immunology | en_US |
dc.subject.mesh | Mitogens - Toxicity | en_US |
dc.subject.mesh | Phytohemagglutinins - Toxicity | en_US |
dc.subject.mesh | Receptors, Interleukin-2 - Drug Effects | en_US |
dc.subject.mesh | Selenium - Pharmacology | en_US |
dc.subject.mesh | Alpha-Tocopherol - Pharmacology | en_US |
dc.title | Immunoregulatory and antioxidant performance of α-tocopherol and selenium on human lymphocytes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, CYJ: jettylee@hku.hk | en_US |
dc.identifier.email | Wan, JMF: jmfwan@hku.hk | en_US |
dc.identifier.authority | Lee, CYJ=rp01511 | en_US |
dc.identifier.authority | Wan, JMF=rp00798 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1385/BTER:86:2:123 | en_US |
dc.identifier.pmid | 12008975 | - |
dc.identifier.scopus | eid_2-s2.0-0036240909 | en_US |
dc.identifier.hkuros | 66208 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036240909&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 86 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 123 | en_US |
dc.identifier.epage | 136 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lee, CYJ=13104265200 | en_US |
dc.identifier.scopusauthorid | Wan, JMF=8930305000 | en_US |
dc.identifier.issnl | 0163-4984 | - |