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Patent History
- ApplicationUS 10/892579 2004-07-16
- PublicationUS 2005084970 2005-04-21
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published patent: Method for constructing and modifying large DNA molecules
Title | Method for constructing and modifying large DNA molecules |
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Priority Date | 2004-07-16 US 10/892579 2003-07-24 US 10/490025P |
Inventors | |
Issue Date | 2005 |
Citation | US Published patent application US 2005084970. Washington, DC: US Patent and Trademark Office (USPTO), 2005 How to Cite? |
Abstract | This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule. |
Persistent Identifier | http://hdl.handle.net/10722/176892 |
DC Field | Value | Language |
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dc.date.accessioned | 2012-11-30T08:38:41Z | - |
dc.date.available | 2012-11-30T08:38:41Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | US Published patent application US 2005084970. Washington, DC: US Patent and Trademark Office (USPTO), 2005 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/176892 | - |
dc.description.abstract | This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule. | en_HK |
dc.relation.isreferencedby | US 7781190 (B2) 2010-08-24 | en_HK |
dc.relation.isreferencedby | US 2005084970 (A1) 2005-04-21 | en_HK |
dc.title | Method for constructing and modifying large DNA molecules | en_HK |
dc.type | Patent | en_US |
dc.description.nature | published_or_final_version | en_US |
dc.contributor.inventor | Huang, J | en_HK |
dc.contributor.inventor | Zhang Xin-Mei | en_HK |
dc.contributor.inventor | Tanner, JA | en_HK |
patents.identifier.application | US 10/892579 | en_HK |
patents.description.assignee | HUANG JIAN-DONG, ; ZHANG XIN-MEI, ; TANNER JULIAN A, ; THE UNIVERSITY OF HONG KONG | en_HK |
patents.description.country | United States of America | en_HK |
patents.date.publication | 2005-04-21 | en_HK |
patents.date.application | 2004-07-16 | en_HK |
patents.date.priority | 2004-07-16 US 10/892579 | en_HK |
patents.date.priority | 2003-07-24 US 10/490025P | en_HK |
patents.description.cc | US | en_HK |
patents.identifier.publication | US 2005084970 | en_HK |
patents.relation.family | US 2005084970 (A1) 2005-04-21 | en_HK |
patents.relation.family | US 7781190 (B2) 2010-08-24 | en_HK |
patents.relation.family | WO 2005010179 (A1) 2005-02-03 | en_HK |
patents.description.kind | A1 | en_HK |
patents.type | Patent_published | en_HK |