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Patent History
- ApplicationUS 10/449801 2003-05-29
- PublicationUS 2004058314 2004-03-25
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published patent: Assay for the detection and quantification of HBV cccDNA by real-time PCR
| Title | Assay for the detection and quantification of HBV cccDNA by real-time PCR |
|---|---|
| Priority Date | 2003-05-29 US 10/449801 2002-05-29 US 10/383953P |
| Inventors | |
| Issue Date | 2004 |
| Citation | US Published patent application US 2004058314. Washington, DC: US Patent and Trademark Office (USPTO), 2004 How to Cite? |
| Abstract | The persistence of covalently closed circular (ccc) DNA of Hepatitis B Virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. A set of primers were designed to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100 to 10<7 >copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy. |
| Persistent Identifier | http://hdl.handle.net/10722/176862 |
| DC Field | Value | Language |
|---|---|---|
| dc.date.accessioned | 2012-11-30T08:38:36Z | - |
| dc.date.available | 2012-11-30T08:38:36Z | - |
| dc.date.issued | 2004 | - |
| dc.identifier.citation | US Published patent application US 2004058314. Washington, DC: US Patent and Trademark Office (USPTO), 2004 | en_HK |
| dc.identifier.uri | http://hdl.handle.net/10722/176862 | - |
| dc.description.abstract | The persistence of covalently closed circular (ccc) DNA of Hepatitis B Virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. A set of primers were designed to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100 to 10<7 >copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy. | en_HK |
| dc.relation.isreferencedby | EP 2396428 (A4) 2012-07-25 | en_HK |
| dc.relation.isreferencedby | EP 2396428 (A2) 2011-12-21 | en_HK |
| dc.relation.isreferencedby | JP 2006217864 (A) 2006-08-24 | en_HK |
| dc.relation.isreferencedby | JP 4708045 (B2) 2011-06-22 | en_HK |
| dc.title | Assay for the detection and quantification of HBV cccDNA by real-time PCR | en_HK |
| dc.type | Patent | en_US |
| dc.description.nature | published_or_final_version | en_US |
| dc.identifier.hkuros | 92190 | - |
| dc.contributor.inventor | He Ming Liang | en_HK |
| dc.contributor.inventor | Kung Hsiang-Fu | en_HK |
| dc.contributor.inventor | Lin, MC | en_HK |
| patents.identifier.application | US 10/449801 | en_HK |
| patents.description.assignee | HE MING LIANG, ; KUNG HSIANG-FU, ; LIN MARIE CHIA MI | en_HK |
| patents.description.country | United States of America | en_HK |
| patents.date.publication | 2004-03-25 | en_HK |
| patents.date.application | 2003-05-29 | en_HK |
| patents.date.priority | 2003-05-29 US 10/449801 | en_HK |
| patents.date.priority | 2002-05-29 US 10/383953P | en_HK |
| patents.description.cc | US | en_HK |
| patents.identifier.publication | US 2004058314 | en_HK |
| patents.relation.family | US 2004058314 (A1) 2004-03-25 | en_HK |
| patents.description.kind | A1 | en_HK |
| patents.type | Patent_published | en_HK |