Cystic fibrosis gene

Abstract

A purified RNA molecule comprises an RNA sequence corresponding to a DNA molecule comprising an intronless DNA sequence selected from: (a) DNA sequences which correspond to the DNA sequence of Figure 1 from amino acid residue position 1 to position 1480; (b) DNA sequences encoding normal CFTR polypeptide having the sequence according to Figure 1 for amino acid residue positions from 1 to 1480; (c) DNA sequences which correspond to a fragment of the sequence of Figure 1 including at least 16 sequential nucleotides between amino acid residue positions 1 and 1480; (d) DNA sequences which comprise at least 16 nucleotides and encode a fragment of amino acid sequence of Figure 1; and (e) DNA sequences encoding an epitope encoded by at least 18 sequential nucleotides in the sequence of Figure 1 between amino acid residue positions 1 and 1480. Similar RNA molecules are also disclosed, as are nucleic acid probes and polypeptides based on the RNA sequences. A method of screening for cystic fibrosis includes use of the nucleotide probes described.