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- Scopus: eid_2-s2.0-0025332587
- PMID: 2156066
- WOS: WOS:A1990CT22200063
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Article: Peripheral type benzodiazepine receptors in human and guinea pig lung: Characterization and autoradiographic mapping
Title | Peripheral type benzodiazepine receptors in human and guinea pig lung: Characterization and autoradiographic mapping |
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Authors | |
Issue Date | 1990 |
Publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org |
Citation | Journal Of Pharmacology And Experimental Therapeutics, 1990, v. 252 n. 2, p. 880-885 How to Cite? |
Abstract | Binding of [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to human and guinea pig lung membranes was characterized and the binding sites localized in lung sections by light microscopic autoradiograpy. Inhibition of [3H]Ro5-4864 binding by unlabeled Ro5-4864, PK11195, midazolam, diazepam, clonazepam and flumazenil indicated that [3H]Ro5-4864 bound to specific receptors and binding was reversible. Scatchard analysis indicated a single class of binding site, with a dissociation constant (K(d)) of 12.8 ± 2.1 nM with maximum binding capacity of 2.6 ± 0.6 pmol/mg of protein for human lung and K(d) of 8.4 ± 1.5 nM and maximum binding capacity of 3.5 ± 0.3 pmol/mg of protein for guinea pig lung. Autoradiograms showed specific labeling over discrete structures in both human and guinea pig lung. Labeling was particularly dense over submucosal glands in intrapulmonary bronchi of human. Airways were also labeled, with epithelium having a higher grain density than smooth muscle. Labeling over smooth muscle was denser in small compared to large airways. Vascular smooth muscle was not labeled in either human and guinea pig. The alveolar walls were uniformly labeled in both species. The functional significance of pulmonary peripheral-type benzodiazepine receptors remains to be determined. |
Persistent Identifier | http://hdl.handle.net/10722/161841 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.829 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Mak, JCW | en_US |
dc.contributor.author | Barnes, PJ | en_US |
dc.date.accessioned | 2012-09-05T05:15:25Z | - |
dc.date.available | 2012-09-05T05:15:25Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Journal Of Pharmacology And Experimental Therapeutics, 1990, v. 252 n. 2, p. 880-885 | en_US |
dc.identifier.issn | 0022-3565 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/161841 | - |
dc.description.abstract | Binding of [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to human and guinea pig lung membranes was characterized and the binding sites localized in lung sections by light microscopic autoradiograpy. Inhibition of [3H]Ro5-4864 binding by unlabeled Ro5-4864, PK11195, midazolam, diazepam, clonazepam and flumazenil indicated that [3H]Ro5-4864 bound to specific receptors and binding was reversible. Scatchard analysis indicated a single class of binding site, with a dissociation constant (K(d)) of 12.8 ± 2.1 nM with maximum binding capacity of 2.6 ± 0.6 pmol/mg of protein for human lung and K(d) of 8.4 ± 1.5 nM and maximum binding capacity of 3.5 ± 0.3 pmol/mg of protein for guinea pig lung. Autoradiograms showed specific labeling over discrete structures in both human and guinea pig lung. Labeling was particularly dense over submucosal glands in intrapulmonary bronchi of human. Airways were also labeled, with epithelium having a higher grain density than smooth muscle. Labeling over smooth muscle was denser in small compared to large airways. Vascular smooth muscle was not labeled in either human and guinea pig. The alveolar walls were uniformly labeled in both species. The functional significance of pulmonary peripheral-type benzodiazepine receptors remains to be determined. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org | en_US |
dc.relation.ispartof | Journal of Pharmacology and Experimental Therapeutics | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Aged | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Autoradiography | en_US |
dc.subject.mesh | Benzodiazepinones - Metabolism | en_US |
dc.subject.mesh | Guinea Pigs | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Isoquinolines - Pharmacology | en_US |
dc.subject.mesh | Lung - Analysis | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Middle Aged | en_US |
dc.subject.mesh | Receptors, Gaba-A - Analysis | en_US |
dc.title | Peripheral type benzodiazepine receptors in human and guinea pig lung: Characterization and autoradiographic mapping | en_US |
dc.type | Article | en_US |
dc.identifier.email | Mak, JCW:judymak@hku.hk | en_US |
dc.identifier.authority | Mak, JCW=rp00352 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 2156066 | - |
dc.identifier.scopus | eid_2-s2.0-0025332587 | en_US |
dc.identifier.volume | 252 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 880 | en_US |
dc.identifier.epage | 885 | en_US |
dc.identifier.isi | WOS:A1990CT22200063 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Mak, JCW=7103323094 | en_US |
dc.identifier.scopusauthorid | Barnes, PJ=36064679400 | en_US |
dc.identifier.issnl | 0022-3565 | - |