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- Publisher Website: 10.1007/s00705-009-0542-2
- Scopus: eid_2-s2.0-75049083912
- PMID: 19911251
- WOS: WOS:000273588000005
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Article: Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins
Title | Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins | ||||||||||
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Authors | |||||||||||
Issue Date | 2010 | ||||||||||
Publisher | Springer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705 | ||||||||||
Citation | Archives Of Virology, 2010, v. 155 n. 1, p. 19-26 How to Cite? | ||||||||||
Abstract | Several peptide mimics of a conserved H5N1 avian influenza virus neutralization site recognized by 8H5 mAb have been reported previously. In this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8H5 mAb. These two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. HEV structural protein p239 and HBc were more suitable than the outer membrane protein T47 of the Treponema pallidum particle for the recovery of reactivity. The increase in the copy number of peptide mimics was important for the recovery of antibody-binding activity and the interaction between peptide and carrier protein may affect the spatial structure of both the peptide and the carrier protein. These results are likely to be of relevance for conformational peptide mimics in diagnostic tests, vaccine and inhibitors. © Springer-Verlag 2009. | ||||||||||
Persistent Identifier | http://hdl.handle.net/10722/125139 | ||||||||||
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.590 | ||||||||||
ISI Accession Number ID |
Funding Information: We acknowledge grants from the Science and Technology Foundation of Fujian Province ( Grant No. 2008Y0059, F2006BAI01B06), the Key Project of Chinese Ministry of Education ( Grant No. 108157), the Foundation from the Ministry of Science and Technology ( Grant No. 2005DC105006), and the Key Project of the Chinese Ministry of Public Health ( Grant No. 2008ZX10004-006). We thank Dr. Anthony ET Yeo for critical review of this manuscript. | ||||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zheng, Z | en_HK |
dc.contributor.author | Luo, W | en_HK |
dc.contributor.author | Song, H | en_HK |
dc.contributor.author | Chen, Y | en_HK |
dc.contributor.author | Xiong, J | en_HK |
dc.contributor.author | Chen, Y | en_HK |
dc.contributor.author | Gu, Y | en_HK |
dc.contributor.author | Chen, H | en_HK |
dc.contributor.author | Shih, JWK | en_HK |
dc.contributor.author | Zhang, J | en_HK |
dc.contributor.author | Xia, N | en_HK |
dc.date.accessioned | 2010-10-31T11:13:36Z | - |
dc.date.available | 2010-10-31T11:13:36Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | Archives Of Virology, 2010, v. 155 n. 1, p. 19-26 | en_HK |
dc.identifier.issn | 0304-8608 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/125139 | - |
dc.description.abstract | Several peptide mimics of a conserved H5N1 avian influenza virus neutralization site recognized by 8H5 mAb have been reported previously. In this study, the secondary and possibly higher structural orders of the peptide mimics 122 and 125 were investigated and found to be closely related to the specific binding with 8H5 mAb. These two peptide mimics were fused to three different carrier proteins, and the antibody binding activities were recovered in 4 of the 11 fusion proteins. HEV structural protein p239 and HBc were more suitable than the outer membrane protein T47 of the Treponema pallidum particle for the recovery of reactivity. The increase in the copy number of peptide mimics was important for the recovery of antibody-binding activity and the interaction between peptide and carrier protein may affect the spatial structure of both the peptide and the carrier protein. These results are likely to be of relevance for conformational peptide mimics in diagnostic tests, vaccine and inhibitors. © Springer-Verlag 2009. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705 | en_HK |
dc.relation.ispartof | Archives of Virology | en_HK |
dc.subject.mesh | Antibodies, Monoclonal - immunology | en_HK |
dc.subject.mesh | Binding Sites, Antibody | en_HK |
dc.subject.mesh | Influenza A Virus, H5N1 Subtype - chemistry - genetics - immunology | en_HK |
dc.subject.mesh | Neutralization Tests | en_HK |
dc.subject.mesh | Peptides - chemistry - genetics - immunology | en_HK |
dc.subject.mesh | Recombinant Fusion Proteins - chemistry - genetics - immunology | en_HK |
dc.title | Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chen, H:hlchen@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chen, H=rp00383 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00705-009-0542-2 | en_HK |
dc.identifier.pmid | 19911251 | - |
dc.identifier.scopus | eid_2-s2.0-75049083912 | en_HK |
dc.identifier.hkuros | 180632 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-75049083912&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 155 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 19 | en_HK |
dc.identifier.epage | 26 | en_HK |
dc.identifier.isi | WOS:000273588000005 | - |
dc.publisher.place | Austria | en_HK |
dc.identifier.scopusauthorid | Zheng, Z=23972049100 | en_HK |
dc.identifier.scopusauthorid | Luo, W=7202199022 | en_HK |
dc.identifier.scopusauthorid | Song, H=36148613900 | en_HK |
dc.identifier.scopusauthorid | Chen, Y=8705548300 | en_HK |
dc.identifier.scopusauthorid | Xiong, J=22837191400 | en_HK |
dc.identifier.scopusauthorid | Chen, Y=10739973700 | en_HK |
dc.identifier.scopusauthorid | Gu, Y=41761534300 | en_HK |
dc.identifier.scopusauthorid | Chen, H=26643315400 | en_HK |
dc.identifier.scopusauthorid | Shih, JWK=7201812989 | en_HK |
dc.identifier.scopusauthorid | Zhang, J=35202802400 | en_HK |
dc.identifier.scopusauthorid | Xia, N=35187953700 | en_HK |
dc.identifier.citeulike | 6186383 | - |
dc.identifier.issnl | 0304-8608 | - |