File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Expression profiling identifies chemokine (C-C motif) ligand 18 as an independent prognostic indicator in gastric cancer

TitleExpression profiling identifies chemokine (C-C motif) ligand 18 as an independent prognostic indicator in gastric cancer
Authors
KeywordsACP
CCL
chemokine (C-C motif) ligand
CI
confidence interval
hazard ratio
HR
IHC
immunohistochemistry
matrix metalloproteinase
MMP
reverse-transcription polymerase chain reaction
RT-PCR
SSC
standard saline citrate
tartrate-resistant acid phosphatase
Issue Date2004
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Gastroenterology, 2004, v. 127 n. 2, p. 457-469 How to Cite?
AbstractBackground & Aims: Gastric cancer is one of the major cancers worldwide. Expression profiling has proven useful in delineating novel prognostic markers in various cancer types. We previously analyzed gene-expression patterns in 90 gastric adenocarcinomas by using complementary DNA microarrays and prioritized a list of genes whose expression levels predict patient outcome. Methods: We identified a specific gene of interest, chemokine (C-C motif) ligand 18 (CCL18), on the basis of a high absolute standardized log Cox hazard ratio, a high variance in expression among all tumor samples, and putative biologic function. Detailed analysis of CCL18 expression with clinicopathologic and survival data was performed (n = 89). Quantitative reverse-transcription polymerase chain reaction was used to verify the microarray expression data and was further applied to analyze an independent cohort of tumor samples (n = 59). The cellular source of CCL18 was determined with immunohistochemistry and in situ hybridization. Results: High CCL18 expression levels were associated with prolonged overall (P = 0.001; hazard ratio, 0.586) and disease-free (P = 0.002; hazard ratio, 0.416) patient survival in the array-based data set by univariate analysis. The observations were confirmed in an independent set of 59 patients by using quantitative reverse-transcription polymerase chain reaction. In multivariate analysis, tumor stage and CCL18 levels were independent prognostic factors for predicting both overall and disease-free survival. We found that CCL18 was expressed by a subpopulation of tumor-associated macrophages that were preferentially located at the tumor invasion front. Conclusions: Macrophage-derived CCL18 may function as a local antitumor immunomodulator that affects patient outcome. Our study suggests CCL18 as a novel candidate for antitumor therapeutics and risk stratification in gastric cancer patients.
Persistent Identifierhttp://hdl.handle.net/10722/88625
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, SYen_HK
dc.contributor.authorYuen, STen_HK
dc.contributor.authorChu, KMen_HK
dc.contributor.authorMathy, JAen_HK
dc.contributor.authorLi, Ren_HK
dc.contributor.authorChan, ASYen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorWong, Jen_HK
dc.contributor.authorChen, Xen_HK
dc.contributor.authorSo, Sen_HK
dc.date.accessioned2010-09-06T09:45:50Z-
dc.date.available2010-09-06T09:45:50Z-
dc.date.issued2004en_HK
dc.identifier.citationGastroenterology, 2004, v. 127 n. 2, p. 457-469en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88625-
dc.description.abstractBackground & Aims: Gastric cancer is one of the major cancers worldwide. Expression profiling has proven useful in delineating novel prognostic markers in various cancer types. We previously analyzed gene-expression patterns in 90 gastric adenocarcinomas by using complementary DNA microarrays and prioritized a list of genes whose expression levels predict patient outcome. Methods: We identified a specific gene of interest, chemokine (C-C motif) ligand 18 (CCL18), on the basis of a high absolute standardized log Cox hazard ratio, a high variance in expression among all tumor samples, and putative biologic function. Detailed analysis of CCL18 expression with clinicopathologic and survival data was performed (n = 89). Quantitative reverse-transcription polymerase chain reaction was used to verify the microarray expression data and was further applied to analyze an independent cohort of tumor samples (n = 59). The cellular source of CCL18 was determined with immunohistochemistry and in situ hybridization. Results: High CCL18 expression levels were associated with prolonged overall (P = 0.001; hazard ratio, 0.586) and disease-free (P = 0.002; hazard ratio, 0.416) patient survival in the array-based data set by univariate analysis. The observations were confirmed in an independent set of 59 patients by using quantitative reverse-transcription polymerase chain reaction. In multivariate analysis, tumor stage and CCL18 levels were independent prognostic factors for predicting both overall and disease-free survival. We found that CCL18 was expressed by a subpopulation of tumor-associated macrophages that were preferentially located at the tumor invasion front. Conclusions: Macrophage-derived CCL18 may function as a local antitumor immunomodulator that affects patient outcome. Our study suggests CCL18 as a novel candidate for antitumor therapeutics and risk stratification in gastric cancer patients.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.subjectACPen_HK
dc.subjectCCLen_HK
dc.subjectchemokine (C-C motif) liganden_HK
dc.subjectCIen_HK
dc.subjectconfidence intervalen_HK
dc.subjecthazard ratioen_HK
dc.subjectHRen_HK
dc.subjectIHCen_HK
dc.subjectimmunohistochemistryen_HK
dc.subjectmatrix metalloproteinaseen_HK
dc.subjectMMPen_HK
dc.subjectreverse-transcription polymerase chain reactionen_HK
dc.subjectRT-PCRen_HK
dc.subjectSSCen_HK
dc.subjectstandard saline citrateen_HK
dc.subjecttartrate-resistant acid phosphataseen_HK
dc.subject.meshAdenocarcinoma - diagnosis - genetics - mortalityen_HK
dc.subject.meshAlgorithmsen_HK
dc.subject.meshAntibodies, Bispecificen_HK
dc.subject.meshChemokines, CC - genetics - immunologyen_HK
dc.subject.meshGastric Mucosa - physiologyen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - immunologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMacrophages - physiologyen_HK
dc.subject.meshPrognosisen_HK
dc.subject.meshStomach Neoplasms - diagnosis - genetics - mortalityen_HK
dc.subject.meshSurvival Analysisen_HK
dc.subject.meshT-Lymphocytes - immunologyen_HK
dc.subject.meshTumor Markers, Biological - geneticsen_HK
dc.titleExpression profiling identifies chemokine (C-C motif) ligand 18 as an independent prognostic indicator in gastric canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=127&issue=2&spage=457&epage=469&date=2004&atitle=Expression+profiling+identifies+chemokine+(C-C+motif)+ligand+18+as+an+independent+prognostic+indicator+in+gastric+canceren_HK
dc.identifier.emailLeung, SY: suetyi@hku.hken_HK
dc.identifier.emailChu, KM: chukm@hkucc.hku.hken_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.authorityLeung, SY=rp00359en_HK
dc.identifier.authorityChu, KM=rp00435en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1053/j.gastro.2004.05.031en_HK
dc.identifier.pmid15300578-
dc.identifier.scopuseid_2-s2.0-4143110195en_HK
dc.identifier.hkuros94921en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4143110195&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume127en_HK
dc.identifier.issue2en_HK
dc.identifier.spage457en_HK
dc.identifier.epage469en_HK
dc.identifier.isiWOS:000223431200017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, SY=7202044886en_HK
dc.identifier.scopusauthoridYuen, ST=7103160927en_HK
dc.identifier.scopusauthoridChu, KM=7402453538en_HK
dc.identifier.scopusauthoridMathy, JA=6701808424en_HK
dc.identifier.scopusauthoridLi, R=7404723915en_HK
dc.identifier.scopusauthoridChan, ASY=7403168075en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK
dc.identifier.scopusauthoridChen, X=8978110800en_HK
dc.identifier.scopusauthoridSo, S=35238727400en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats