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Article: Hepatic stress gene expression and ultrastructural features under intermittent Pringle manoeuvre

TitleHepatic stress gene expression and ultrastructural features under intermittent Pringle manoeuvre
Authors
KeywordsGene expression
Pringle manoeuvre
Stress
Ultrastructure
Issue Date2002
PublisherThe First Affiliated Hospital, Zhejiang University School of Medicine. The Journal's web site is located at http://www.hbpdint.com/
Citation
Hepatobiliary And Pancreatic Diseases International, 2002, v. 1 n. 2, p. 249-257 How to Cite?
AbstractBackground: Intermittent Pringle manoeuvre resulted in less blood loss and better preservation of liver function when it was applied for fewer than 120 minutes. The mechanism of better preservation of liver function under intermittent Pringle manoeuvre at molecular level remains unclear. Furthermore, the ultrastructural features in the liver with chronic diseases under intermittent Pringle manoeuvre have not been studied. The aim of the study is to investigate the expression of stress genes and ultrastructural change of the liver under intermittent Pringle manoeuvre. Methods: From July 1995 to February 1998, 131 patients underwent hepatectomy for liver tumours (61 patients without Pringle manoeuvre and 70 patients with intermittent Pringle manoeuvre). Twenty-five patients (15 with Pringle manoeuvre and 10 without Pringle manoeuvre) were included in the study of hepatic stress gene expression during hepatectomy. Twenty-two patients (18 patients with intermittent Pringle manoeuvre and four patients without Pringle manoeuvre) were randomly assigned for electron microscopic examination. Results: For the expression of stress genes, both the heat shock genes (HSP 70A and HSC 70) and acute phase genes (TNF-α and interleukin-6) were detected simultaneously in the patients with or without intermittent Pringle manoeuvre. The patients under intermittent Pringle manoeuvre had relatively higher mRNA levels of heat shock gene 70 family, which is related to intracellular repair and protection. Induction of TNF-α and interleukin-6 genes, which contributed to ischaemia-reperfusion injury and postoperative complication, was not found in the patients under intermittent Pringle manoeuvre. Under the electron microscopy, the hepatic ultrastructure was well maintained under intermittent Pringle manoeuvre whatever the liver status, even when the accumulated ischaemic duration was extended to 120 min. Conclusion: Intermittent Pringle manoeuvre induced relatively higher expression of heat shock genes, which are related to intracellular homeostasis, and is consistent with the well maintenance of liver ultrastructure.
Persistent Identifierhttp://hdl.handle.net/10722/88451
ISSN
2015 Impact Factor: 1.724
2015 SCImago Journal Rankings: 0.717
References

 

DC FieldValueLanguage
dc.contributor.authorMan, Ken_HK
dc.contributor.authorLiang, TBen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorLiu, CLen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorYu, WCen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-06T09:43:34Z-
dc.date.available2010-09-06T09:43:34Z-
dc.date.issued2002en_HK
dc.identifier.citationHepatobiliary And Pancreatic Diseases International, 2002, v. 1 n. 2, p. 249-257en_HK
dc.identifier.issn1499-3872en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88451-
dc.description.abstractBackground: Intermittent Pringle manoeuvre resulted in less blood loss and better preservation of liver function when it was applied for fewer than 120 minutes. The mechanism of better preservation of liver function under intermittent Pringle manoeuvre at molecular level remains unclear. Furthermore, the ultrastructural features in the liver with chronic diseases under intermittent Pringle manoeuvre have not been studied. The aim of the study is to investigate the expression of stress genes and ultrastructural change of the liver under intermittent Pringle manoeuvre. Methods: From July 1995 to February 1998, 131 patients underwent hepatectomy for liver tumours (61 patients without Pringle manoeuvre and 70 patients with intermittent Pringle manoeuvre). Twenty-five patients (15 with Pringle manoeuvre and 10 without Pringle manoeuvre) were included in the study of hepatic stress gene expression during hepatectomy. Twenty-two patients (18 patients with intermittent Pringle manoeuvre and four patients without Pringle manoeuvre) were randomly assigned for electron microscopic examination. Results: For the expression of stress genes, both the heat shock genes (HSP 70A and HSC 70) and acute phase genes (TNF-α and interleukin-6) were detected simultaneously in the patients with or without intermittent Pringle manoeuvre. The patients under intermittent Pringle manoeuvre had relatively higher mRNA levels of heat shock gene 70 family, which is related to intracellular repair and protection. Induction of TNF-α and interleukin-6 genes, which contributed to ischaemia-reperfusion injury and postoperative complication, was not found in the patients under intermittent Pringle manoeuvre. Under the electron microscopy, the hepatic ultrastructure was well maintained under intermittent Pringle manoeuvre whatever the liver status, even when the accumulated ischaemic duration was extended to 120 min. Conclusion: Intermittent Pringle manoeuvre induced relatively higher expression of heat shock genes, which are related to intracellular homeostasis, and is consistent with the well maintenance of liver ultrastructure.en_HK
dc.languageengen_HK
dc.publisherThe First Affiliated Hospital, Zhejiang University School of Medicine. The Journal's web site is located at http://www.hbpdint.com/en_HK
dc.relation.ispartofHepatobiliary and Pancreatic Diseases Internationalen_HK
dc.subjectGene expressionen_HK
dc.subjectPringle manoeuvreen_HK
dc.subjectStressen_HK
dc.subjectUltrastructureen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshHSP70 Heat-Shock Proteins - genetics - metabolismen_HK
dc.subject.meshHemostasis, Surgicalen_HK
dc.subject.meshHepatectomyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInterleukin-6 - genetics - metabolismen_HK
dc.subject.meshLiver - metabolism - pathologyen_HK
dc.subject.meshLiver Neoplasms - metabolism - pathology - surgeryen_HK
dc.subject.meshMicroscopy, Electronen_HK
dc.subject.meshTumor Necrosis Factor-alpha - genetics - metabolismen_HK
dc.titleHepatic stress gene expression and ultrastructural features under intermittent Pringle manoeuvreen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1499-3872&volume=1&issue=2&spage=249&epage=257&date=2002&atitle=Hepatic+stress+gene+expression+and+ultrastructural+features+under+intermittent+pringle+manoeuvreen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailNg, IOL: iolng@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid14612278-
dc.identifier.scopuseid_2-s2.0-0037868065en_HK
dc.identifier.hkuros68934en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037868065&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1en_HK
dc.identifier.issue2en_HK
dc.identifier.spage249en_HK
dc.identifier.epage257en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridLiang, TB=7202019213en_HK
dc.identifier.scopusauthoridLo, CM=7401771672en_HK
dc.identifier.scopusauthoridLiu, CL=7409789712en_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.scopusauthoridYu, WC=37022285400en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK

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