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Article: Expression of aquaporin-3 in human peritoneal mesothelial cells and its up-regulation by glucose in vitro

TitleExpression of aquaporin-3 in human peritoneal mesothelial cells and its up-regulation by glucose in vitro
Authors
KeywordsAQP-3
CAPD
Human peritoneal mesothelial cell
Osmolality
Peritoneum
Three pore model
Ultrasmall pores
Water channel
Issue Date2002
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ki/index.html
Citation
Kidney International, 2002, v. 62 n. 4, p. 1431-1439 How to Cite?
AbstractBackground. Aquaporin-3 (AQP3) is a member of the water channel family that is selective for the passage of not only water, but also glycerol and urea. Our recent study demonstrated the presence of aquaporin-1 in human peritoneal mesothelial cells (HPMC). Although transcripts encoding for AQP3 has been detected by reverse transcription-polymerase chain reaction (RT-PCR) in murine peritoneal mesothelium, to date there is no documentation of protein expression on peritoneal mesothelial cells. Method. Our present study was designed to explore the gene and protein expression of AQP3 in HPMC and its regulation under different concentrations of glucose. Results. AQP3 protein was detected in the human peritoneal tissue by immunohistological staining using specific, affinity-purified polyclonal anti-AQP3 antibodies. AQ3 transcripts and protein expression in cultured HPMC were investigated by RT-PCR and immunoblotting analysis respectively. Cell permeability to glycerol (flux) was measured using [14C]glycerol incorporation. AQP3 transcript and protein were weakly expressed in HPMC constitutively. The gene expression of AQP3 and its protein biosynthesis in HPMC were inducible following exposure to glucose in a dose- and time-dependent manner (P < 0.0001). Glucose at a concentration of 200 mmol induced glycerol flux by 4.82-fold above the control value (P < 0.0001) and its effect was significantly inhibited by mercuric chloride (P ± 0.01). Conclusion. Our novel observation demonstrated the AQP3 expression and biosynthesis in HPMC and in vitro studies revealed that glycerol permeability in HPMC was up-regulated by glucose. Further study is warranted to elucidate the role of AQP3 in HPMC for maintaining the ultrafiltration of the peritoneal membrane.
Persistent Identifierhttp://hdl.handle.net/10722/78692
ISSN
2015 Impact Factor: 7.683
2015 SCImago Journal Rankings: 3.181
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, LYYen_HK
dc.contributor.authorTang, Sen_HK
dc.contributor.authorLi, FKen_HK
dc.contributor.authorLui, SLen_HK
dc.contributor.authorChan, TMen_HK
dc.date.accessioned2010-09-06T07:45:41Z-
dc.date.available2010-09-06T07:45:41Z-
dc.date.issued2002en_HK
dc.identifier.citationKidney International, 2002, v. 62 n. 4, p. 1431-1439en_HK
dc.identifier.issn0085-2538en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78692-
dc.description.abstractBackground. Aquaporin-3 (AQP3) is a member of the water channel family that is selective for the passage of not only water, but also glycerol and urea. Our recent study demonstrated the presence of aquaporin-1 in human peritoneal mesothelial cells (HPMC). Although transcripts encoding for AQP3 has been detected by reverse transcription-polymerase chain reaction (RT-PCR) in murine peritoneal mesothelium, to date there is no documentation of protein expression on peritoneal mesothelial cells. Method. Our present study was designed to explore the gene and protein expression of AQP3 in HPMC and its regulation under different concentrations of glucose. Results. AQP3 protein was detected in the human peritoneal tissue by immunohistological staining using specific, affinity-purified polyclonal anti-AQP3 antibodies. AQ3 transcripts and protein expression in cultured HPMC were investigated by RT-PCR and immunoblotting analysis respectively. Cell permeability to glycerol (flux) was measured using [14C]glycerol incorporation. AQP3 transcript and protein were weakly expressed in HPMC constitutively. The gene expression of AQP3 and its protein biosynthesis in HPMC were inducible following exposure to glucose in a dose- and time-dependent manner (P < 0.0001). Glucose at a concentration of 200 mmol induced glycerol flux by 4.82-fold above the control value (P < 0.0001) and its effect was significantly inhibited by mercuric chloride (P ± 0.01). Conclusion. Our novel observation demonstrated the AQP3 expression and biosynthesis in HPMC and in vitro studies revealed that glycerol permeability in HPMC was up-regulated by glucose. Further study is warranted to elucidate the role of AQP3 in HPMC for maintaining the ultrafiltration of the peritoneal membrane.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ki/index.htmlen_HK
dc.relation.ispartofKidney Internationalen_HK
dc.subjectAQP-3en_HK
dc.subjectCAPDen_HK
dc.subjectHuman peritoneal mesothelial cellen_HK
dc.subjectOsmolalityen_HK
dc.subjectPeritoneumen_HK
dc.subjectThree pore modelen_HK
dc.subjectUltrasmall poresen_HK
dc.subjectWater channelen_HK
dc.subject.meshAquaporin 3en_HK
dc.subject.meshAquaporins - analysis - geneticsen_HK
dc.subject.meshCarbon Radioisotopes - diagnostic useen_HK
dc.subject.meshCell Membrane Permeability - drug effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDiuretics, Osmotic - pharmacologyen_HK
dc.subject.meshEpithelial Cells - chemistry - cytology - physiologyen_HK
dc.subject.meshGene Expression - drug effectsen_HK
dc.subject.meshGlucose - pharmacologyen_HK
dc.subject.meshGlycerol - pharmacokineticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoblottingen_HK
dc.subject.meshMannitol - pharmacologyen_HK
dc.subject.meshPeritoneum - cytologyen_HK
dc.subject.meshUp-Regulation - drug effectsen_HK
dc.titleExpression of aquaporin-3 in human peritoneal mesothelial cells and its up-regulation by glucose in vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0085-2538&volume=62&spage=1431&epage=1439&date=2002&atitle=Expression+of+Aquaporin-3+in+Human+Peritoneal+Mesothelial+Cells+and+Its+Up-regulation+by+Glucose+in+Vitroen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.emailTang, S: scwtang@hku.hken_HK
dc.identifier.emailChan, TM: dtmchan@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityTang, S=rp00480en_HK
dc.identifier.authorityChan, TM=rp00394en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1523-1755.2002.00564.xen_HK
dc.identifier.pmid12234316-
dc.identifier.scopuseid_2-s2.0-0036378211en_HK
dc.identifier.hkuros81136en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036378211&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume62en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1431en_HK
dc.identifier.epage1439en_HK
dc.identifier.isiWOS:000178042000036-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridChan, LYY=8108378300en_HK
dc.identifier.scopusauthoridTang, S=7403437082en_HK
dc.identifier.scopusauthoridLi, FK=8219093900en_HK
dc.identifier.scopusauthoridLui, SL=7102379130en_HK
dc.identifier.scopusauthoridChan, TM=7402687700en_HK

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