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Article: Expression of aquaporin-1 in human peritoneal mesothelial cells and its upregulation by glucose In vitro

TitleExpression of aquaporin-1 in human peritoneal mesothelial cells and its upregulation by glucose In vitro
Authors
Issue Date2001
PublisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org
Citation
Journal Of The American Society Of Nephrology, 2001, v. 12 n. 5, p. 1036-1045 How to Cite?
AbstractAquaporin (AQP) is a family of water channels that are highly selective for the passage of water and occasionally glycerol. In previous studies, only AQP1 was found in human peritoneal endothelial cells in both control subjects and patients on peritoneal dialysis. As human peritoneal mesothelial cells (HPMC) play an important role in dialysis adequacy and fluid balance in continuous ambulatory peritoneal dialysis patients, this study examined whether AQP1 is present in HPMC. It was found that AQP1 mRNA and protein are present in HPMC constitutively. The localization of AQP1 protein in peritoneal mesothelial cells was confirmed by double immunohistochemical staining of the mesothelial lining of human peritoneal membrane. More important, the expression of AQP1 in HPMC is not constitutive and the transcription and biosynthesis of AQP1 in HPMC is inducible by osmotic agents such as glucose and mannitol. There was significant enhancement of AQP1 biosynthesis upon exposure to glucose in a time- and dose-dependent manner (P < 0.0001). Similar findings were observed in the AQP1 biosynthesis by an endothelial cell line, EA.hy 926. Of particular interest, the upregulation in AQP1 mRNA or biosynthesis in mesothelial cells was always significantly higher than that of endothelial cells when the experiments were conducted under identical settings (P < 0.001). AQP1 expression in HPMC was demonstrated for the first time. Osmotic agents upregulate both mRNA and protein expression of this aquaporin. The role of AQP1 in HPMC in maintaining the ultrafiltration of the peritoneal membrane is potentially of clinical interest.
Persistent Identifierhttp://hdl.handle.net/10722/77343
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorFu Keung Lien_HK
dc.contributor.authorHao Yui Lanen_HK
dc.contributor.authorTang, Sen_HK
dc.contributor.authorTsang, AWLen_HK
dc.contributor.authorChan, DTMen_HK
dc.contributor.authorLeung, JCen_HK
dc.date.accessioned2010-09-06T07:30:53Z-
dc.date.available2010-09-06T07:30:53Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of The American Society Of Nephrology, 2001, v. 12 n. 5, p. 1036-1045en_HK
dc.identifier.issn1046-6673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77343-
dc.description.abstractAquaporin (AQP) is a family of water channels that are highly selective for the passage of water and occasionally glycerol. In previous studies, only AQP1 was found in human peritoneal endothelial cells in both control subjects and patients on peritoneal dialysis. As human peritoneal mesothelial cells (HPMC) play an important role in dialysis adequacy and fluid balance in continuous ambulatory peritoneal dialysis patients, this study examined whether AQP1 is present in HPMC. It was found that AQP1 mRNA and protein are present in HPMC constitutively. The localization of AQP1 protein in peritoneal mesothelial cells was confirmed by double immunohistochemical staining of the mesothelial lining of human peritoneal membrane. More important, the expression of AQP1 in HPMC is not constitutive and the transcription and biosynthesis of AQP1 in HPMC is inducible by osmotic agents such as glucose and mannitol. There was significant enhancement of AQP1 biosynthesis upon exposure to glucose in a time- and dose-dependent manner (P < 0.0001). Similar findings were observed in the AQP1 biosynthesis by an endothelial cell line, EA.hy 926. Of particular interest, the upregulation in AQP1 mRNA or biosynthesis in mesothelial cells was always significantly higher than that of endothelial cells when the experiments were conducted under identical settings (P < 0.001). AQP1 expression in HPMC was demonstrated for the first time. Osmotic agents upregulate both mRNA and protein expression of this aquaporin. The role of AQP1 in HPMC in maintaining the ultrafiltration of the peritoneal membrane is potentially of clinical interest.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.orgen_HK
dc.relation.ispartofJournal of the American Society of Nephrologyen_HK
dc.subject.meshAquaporin 1en_HK
dc.subject.meshAquaporins - genetics - metabolismen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBlood Group Antigensen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDNA Primers - geneticsen_HK
dc.subject.meshEpithelial Cells - drug effects - metabolismen_HK
dc.subject.meshGlucose - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPeritoneal Cavity - cytologyen_HK
dc.subject.meshPeritoneum - drug effects - metabolismen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshUp-Regulation - drug effectsen_HK
dc.titleExpression of aquaporin-1 in human peritoneal mesothelial cells and its upregulation by glucose In vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1046-6673&volume=12&issue=5&spage=1036&epage=1045&date=2001&atitle=Expression+of+aquaporin-1+in+human+peritoneal+mesothelial+cells+and+its+upregulation+by+glucose+in+vitroen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailTang, S: scwtang@hku.hken_HK
dc.identifier.emailChan, DTM: dtmchan@hku.hken_HK
dc.identifier.emailLeung, JC: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityTang, S=rp00480en_HK
dc.identifier.authorityChan, DTM=rp00394en_HK
dc.identifier.authorityLeung, JC=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11316863-
dc.identifier.scopuseid_2-s2.0-0035035681en_HK
dc.identifier.hkuros59916en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035035681&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1036en_HK
dc.identifier.epage1045en_HK
dc.identifier.isiWOS:000168307600020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridFu Keung Li=55210612400en_HK
dc.identifier.scopusauthoridHao Yui Lan=7409625014en_HK
dc.identifier.scopusauthoridTang, S=7403437082en_HK
dc.identifier.scopusauthoridTsang, AWL=7006979244en_HK
dc.identifier.scopusauthoridChan, DTM=7402687700en_HK
dc.identifier.scopusauthoridLeung, JC=7202180349en_HK

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