File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Increased survival of mesothelial cells from the peritoneum in peritoneal dialysis fluid

TitleIncreased survival of mesothelial cells from the peritoneum in peritoneal dialysis fluid
Authors
KeywordsCAPD
Cell viability
Glucose
Mesothelial cells
Peritoneum
PH
Issue Date2001
PublisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm
Citation
Cell Biology International, 2001, v. 25 n. 5, p. 445-450 How to Cite?
AbstractPeritonitis remains the most important factor in patient morbidity and technical failure associated with continuous ambulatory peritoneal dialysis (CAPD). In vitro examination of bacterial infection of cultured human peritoneal mesothelial cells (HPMC) is an attractive approach to the study of peritonitis in CAPD, yet there are few reports on this subject. Previous studies have shown two limitations: (i) cell cultures of HPMC lasted for days only when incubated in culture medium and (ii) short-term studies of < 30 min were done in HPMC when incubated with peritoneal dialysis fluid (PDF). Human peritoneal mesothelial cells, maintained in a conventional single chamber culture system with PDF alone, were unable to survive more than 40 min. The present study was designed to prolong the viability of HPMC cultured in PDF, with the object of using cells under different conditions, such as that of simulating CAPD. HPMC were cultured using plastic microtiter plates, where they were grown to confluence and growth was arrested. PDF containing different concentrations of NaHCO 3 and human serum albumin was added. Cell viability after exposure for up to 24 h was measured by trypan blue, Cell Death Detection ELISA and Annex-V flow cytometry. The data confirmed the 'toxic' effect of PDF, with cell viability being <40% after 2 h incubation in 4.25% glucose in PDF. However, the survival time of HPMC increased significantly in 4.25% glucose PDF at a physiological pH and even further after the addition of human albumin. These experimental conditions simulating CAPD may allow future in vitro studies of mesothelial physiology and peritonitis related to CAPD treatment. © 2001 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/76230
ISSN
2015 Impact Factor: 1.663
2015 SCImago Journal Rankings: 0.705
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorHo, SKNen_HK
dc.contributor.authorLeung, Jen_HK
dc.contributor.authorTang, SCWen_HK
dc.contributor.authorChan, TMen_HK
dc.contributor.authorLi, FKen_HK
dc.date.accessioned2010-09-06T07:19:00Z-
dc.date.available2010-09-06T07:19:00Z-
dc.date.issued2001en_HK
dc.identifier.citationCell Biology International, 2001, v. 25 n. 5, p. 445-450en_HK
dc.identifier.issn1065-6995en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76230-
dc.description.abstractPeritonitis remains the most important factor in patient morbidity and technical failure associated with continuous ambulatory peritoneal dialysis (CAPD). In vitro examination of bacterial infection of cultured human peritoneal mesothelial cells (HPMC) is an attractive approach to the study of peritonitis in CAPD, yet there are few reports on this subject. Previous studies have shown two limitations: (i) cell cultures of HPMC lasted for days only when incubated in culture medium and (ii) short-term studies of < 30 min were done in HPMC when incubated with peritoneal dialysis fluid (PDF). Human peritoneal mesothelial cells, maintained in a conventional single chamber culture system with PDF alone, were unable to survive more than 40 min. The present study was designed to prolong the viability of HPMC cultured in PDF, with the object of using cells under different conditions, such as that of simulating CAPD. HPMC were cultured using plastic microtiter plates, where they were grown to confluence and growth was arrested. PDF containing different concentrations of NaHCO 3 and human serum albumin was added. Cell viability after exposure for up to 24 h was measured by trypan blue, Cell Death Detection ELISA and Annex-V flow cytometry. The data confirmed the 'toxic' effect of PDF, with cell viability being <40% after 2 h incubation in 4.25% glucose in PDF. However, the survival time of HPMC increased significantly in 4.25% glucose PDF at a physiological pH and even further after the addition of human albumin. These experimental conditions simulating CAPD may allow future in vitro studies of mesothelial physiology and peritonitis related to CAPD treatment. © 2001 Academic Press.en_HK
dc.languageengen_HK
dc.publisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htmen_HK
dc.relation.ispartofCell Biology Internationalen_HK
dc.subjectCAPDen_HK
dc.subjectCell viabilityen_HK
dc.subjectGlucoseen_HK
dc.subjectMesothelial cellsen_HK
dc.subjectPeritoneumen_HK
dc.subjectPHen_HK
dc.subject.meshCell Culture Techniques - methodsen_HK
dc.subject.meshCell Survival - drug effectsen_HK
dc.subject.meshColoring Agentsen_HK
dc.subject.meshDialysis Solutions - pharmacologyen_HK
dc.subject.meshEpithelial Cells - cytologyen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshGlucose - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydrogen-Ion Concentrationen_HK
dc.subject.meshKidney Failure, Chronic - pathology - therapyen_HK
dc.subject.meshPeritoneal Dialysis, Continuous Ambulatoryen_HK
dc.subject.meshPeritoneum - cytologyen_HK
dc.subject.meshPeritonitis - pathologyen_HK
dc.subject.meshTrypan Blueen_HK
dc.titleIncreased survival of mesothelial cells from the peritoneum in peritoneal dialysis fluiden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1065-6995&volume=25&issue=5&spage=445&epage=450&date=2001&atitle=Increased+survival+of+mesothelial+cells+from+the+peritoneum+in+peritoneal+dialysis+fluiden_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailTang, SCW: scwtang@hku.hken_HK
dc.identifier.emailChan, TM: dtmchan@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityTang, SCW=rp00480en_HK
dc.identifier.authorityChan, TM=rp00394en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/cbir.2000.0664en_HK
dc.identifier.pmid11401332-
dc.identifier.scopuseid_2-s2.0-0034982573en_HK
dc.identifier.hkuros59914en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034982573&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue5en_HK
dc.identifier.spage445en_HK
dc.identifier.epage450en_HK
dc.identifier.isiWOS:000169053700007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridHo, SKN=36839065300en_HK
dc.identifier.scopusauthoridLeung, J=7202180353en_HK
dc.identifier.scopusauthoridTang, SCW=7403437082en_HK
dc.identifier.scopusauthoridChan, TM=7402687700en_HK
dc.identifier.scopusauthoridLi, FK=8219093900en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats