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Article: Association of MAD2 expression with mitotic checkpoint competence in hepatoma cells

TitleAssociation of MAD2 expression with mitotic checkpoint competence in hepatoma cells
Authors
KeywordsCarcinogenesis
Cell cycle control
Chromosomal instability
Genome instability
Hepatocellular carcinoma
MAD2
Microtubule toxin
Mitotic checkpoint
Spindle assembly checkpoint
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1021-7770
Citation
Journal Of Biomedical Science, 2004, v. 11 n. 6, p. 920-927 How to Cite?
AbstractChromosomal instability (CIN) refers to high rates of chromosomal gains and losses and is a major cause of genomic instability of cells. It is thought that CIN caused by loss of mitotic checkpoint contributes to carcinogenesis. In this study, we evaluated the competence of mitotic checkpoint in hepatoma cells and investigated the cause of mitotic checkpoint defects. We found that 6 (54.5%) of the 11 hepatoma cell lines were defective in mitotic checkpoint control as monitored by mitotic indices and flow-cytometric analysis after treatment with microtubule toxins. Interestingly, all 6 hepatoma cell lines with defective mitotic checkpoint showed significant underexpression of mitotic arrest deficient 2 (MAD2), a key mitotic checkpoint protein. The level of MAD2 underexpression was significantly associated with defective mitotic checkpoint response (p < 0.001). In addition, no mutations were found in the coding sequences of MAD2 in all 11 hepatoma cell lines. Our findings suggest that MAD2 deficiency may cause a mitotic checkpoint defect in hepatoma cells. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/68037
ISSN
2015 Impact Factor: 2.935
2015 SCImago Journal Rankings: 1.280
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSze, KMFen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorJin, DYen_HK
dc.contributor.authorNg, IOLen_HK
dc.date.accessioned2010-09-06T06:00:44Z-
dc.date.available2010-09-06T06:00:44Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Biomedical Science, 2004, v. 11 n. 6, p. 920-927en_HK
dc.identifier.issn1021-7770en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68037-
dc.description.abstractChromosomal instability (CIN) refers to high rates of chromosomal gains and losses and is a major cause of genomic instability of cells. It is thought that CIN caused by loss of mitotic checkpoint contributes to carcinogenesis. In this study, we evaluated the competence of mitotic checkpoint in hepatoma cells and investigated the cause of mitotic checkpoint defects. We found that 6 (54.5%) of the 11 hepatoma cell lines were defective in mitotic checkpoint control as monitored by mitotic indices and flow-cytometric analysis after treatment with microtubule toxins. Interestingly, all 6 hepatoma cell lines with defective mitotic checkpoint showed significant underexpression of mitotic arrest deficient 2 (MAD2), a key mitotic checkpoint protein. The level of MAD2 underexpression was significantly associated with defective mitotic checkpoint response (p < 0.001). In addition, no mutations were found in the coding sequences of MAD2 in all 11 hepatoma cell lines. Our findings suggest that MAD2 deficiency may cause a mitotic checkpoint defect in hepatoma cells. Copyright © 2004 National Science Council, ROC and S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1021-7770en_HK
dc.relation.ispartofJournal of Biomedical Scienceen_HK
dc.subjectCarcinogenesisen_HK
dc.subjectCell cycle controlen_HK
dc.subjectChromosomal instabilityen_HK
dc.subjectGenome instabilityen_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subjectMAD2en_HK
dc.subjectMicrotubule toxinen_HK
dc.subjectMitotic checkpointen_HK
dc.subjectSpindle assembly checkpointen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCalcium-Binding Proteins - metabolism - physiologyen_HK
dc.subject.meshCarcinoma, Hepatocellular - metabolismen_HK
dc.subject.meshCell Cycle Proteinsen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromosomal Instabilityen_HK
dc.subject.meshDNA Mutational Analysisen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGenomeen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLiver Neoplasms - metabolismen_HK
dc.subject.meshMicroscopy, Fluorescenceen_HK
dc.subject.meshMicrotubules - metabolismen_HK
dc.subject.meshMitosisen_HK
dc.subject.meshMitotic Spindle Apparatusen_HK
dc.subject.meshRepressor Proteinsen_HK
dc.subject.meshTime Factorsen_HK
dc.titleAssociation of MAD2 expression with mitotic checkpoint competence in hepatoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1021-7770&volume=11&spage=920&epage=7&date=2004&atitle=Association+of+MAD2+expression+with+mitotic+checkpoint+competence+in+hepatoma+cellsen_HK
dc.identifier.emailChing, YP:ypching@hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000081839en_HK
dc.identifier.pmid15591789-
dc.identifier.scopuseid_2-s2.0-10844233849en_HK
dc.identifier.hkuros102270en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10844233849&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue6en_HK
dc.identifier.spage920en_HK
dc.identifier.epage927en_HK
dc.identifier.isiWOS:000225547000024-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridSze, KMF=36828094800en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK

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