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Article: Metastatic Trophoblastic Disease after an Initial Diagnosis of Partial Hydatidiform Mole: Genotyping and Chromosome In Situ Hybridization Analysis

TitleMetastatic Trophoblastic Disease after an Initial Diagnosis of Partial Hydatidiform Mole: Genotyping and Chromosome In Situ Hybridization Analysis
Authors
KeywordsChromosome in situ hybridization
Genotyping
Gestational trophoblastic disease
Metastatic partial mole
Issue Date2004
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 2004, v. 100 n. 7, p. 1411-1417 How to Cite?
AbstractBACKGROUND. Hydatidiform mole (HM) is classified into partial (PHM) and complete (CHM) subtypes according to histopathologic and genetic criteria. Traditionally, it is believed that PHM carries a better prognosis and rarely develops metastasis. However, making a distinction between PHM and CHM using histologic criteria alone may be difficult. METHODS. The authors used fluorescent microsatellite genotyping following laser-capture microdissection and chromosome in situ hybridization (CISH) to perform a genetic analysis of six patients with histologically diagnosed PHM who subsequently developed metastatic gestational trophoblastic neoplasia. RESULTS. Patients ranged in age from 25 years to 44 years (mean, 33.2 years). The gestational age of the molar pregnancies varied from 6 weeks to 20 weeks. All six patients had pulmonary metastases, with additional liver metastasis in two patients. Among the six patients with histologically diagnosed PHM, it was found that four patients had a diploid karyotype and no maternal alleles; thus, their neoplasms actually were CHM. Maternal genome was detected in the remaining two patients consistent with a biparental origin, and these patients had a triploid karyotype. CISH findings in all patients correlated with the genotyping findings. Triploid HM had maternally derived alleles, whereas diploid HMs were purely androgenetic. CONCLUSIONS. In the current study, which may be the largest series of genetically analyzed metastatic PHMs to date, the difficulty of histologic distinction between PHM and CHM was confirmed. Molecular analysis may help to refine the classification of HM. Although the current findings support the belief that most aggressive trophoblastic diseases are derived from CHM, a small number of PHMs do progress to metastatic disease. Thus, the current study reaffirmed that all patients with HM should be followed closely irrespective of histologic subclassification. © 2004 American Cancer Society.
Persistent Identifierhttp://hdl.handle.net/10722/67604
ISSN
2015 Impact Factor: 5.649
2015 SCImago Journal Rankings: 3.188
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorKhoo, USen_HK
dc.contributor.authorLai, CYLen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorXue, WCen_HK
dc.contributor.authorCheng, DKLen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T05:56:36Z-
dc.date.available2010-09-06T05:56:36Z-
dc.date.issued2004en_HK
dc.identifier.citationCancer, 2004, v. 100 n. 7, p. 1411-1417en_HK
dc.identifier.issn0008-543Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67604-
dc.description.abstractBACKGROUND. Hydatidiform mole (HM) is classified into partial (PHM) and complete (CHM) subtypes according to histopathologic and genetic criteria. Traditionally, it is believed that PHM carries a better prognosis and rarely develops metastasis. However, making a distinction between PHM and CHM using histologic criteria alone may be difficult. METHODS. The authors used fluorescent microsatellite genotyping following laser-capture microdissection and chromosome in situ hybridization (CISH) to perform a genetic analysis of six patients with histologically diagnosed PHM who subsequently developed metastatic gestational trophoblastic neoplasia. RESULTS. Patients ranged in age from 25 years to 44 years (mean, 33.2 years). The gestational age of the molar pregnancies varied from 6 weeks to 20 weeks. All six patients had pulmonary metastases, with additional liver metastasis in two patients. Among the six patients with histologically diagnosed PHM, it was found that four patients had a diploid karyotype and no maternal alleles; thus, their neoplasms actually were CHM. Maternal genome was detected in the remaining two patients consistent with a biparental origin, and these patients had a triploid karyotype. CISH findings in all patients correlated with the genotyping findings. Triploid HM had maternally derived alleles, whereas diploid HMs were purely androgenetic. CONCLUSIONS. In the current study, which may be the largest series of genetically analyzed metastatic PHMs to date, the difficulty of histologic distinction between PHM and CHM was confirmed. Molecular analysis may help to refine the classification of HM. Although the current findings support the belief that most aggressive trophoblastic diseases are derived from CHM, a small number of PHMs do progress to metastatic disease. Thus, the current study reaffirmed that all patients with HM should be followed closely irrespective of histologic subclassification. © 2004 American Cancer Society.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_HK
dc.relation.ispartofCanceren_HK
dc.rightsCancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectChromosome in situ hybridizationen_HK
dc.subjectGenotypingen_HK
dc.subjectGestational trophoblastic diseaseen_HK
dc.subjectMetastatic partial moleen_HK
dc.subject.meshAdulten_HK
dc.subject.meshCytogenetic Analysis - methodsen_HK
dc.subject.meshDiagnosis, Differentialen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydatidiform Mole - genetics - pathology - surgeryen_HK
dc.subject.meshMicrosatellite Repeats - geneticsen_HK
dc.subject.meshNeoplasm Metastasisen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshRetrospective Studiesen_HK
dc.subject.meshUterine Neoplasms - genetics - pathology - surgeryen_HK
dc.titleMetastatic Trophoblastic Disease after an Initial Diagnosis of Partial Hydatidiform Mole: Genotyping and Chromosome In Situ Hybridization Analysisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-543X&volume=100&issue=7&spage=1411&epage=1417&date=2004&atitle=Metastatic+trophoblastic+disease+after+an+initial+diagnosis+of+partial+hydatidiform+mole:+genotyping+and+chromosome+in+situ+hybridization+analysisen_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailKhoo, US: uskhoo@hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityKhoo, US=rp00362en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/cncr.20107en_HK
dc.identifier.pmid15042675-
dc.identifier.scopuseid_2-s2.0-1642348690en_HK
dc.identifier.hkuros86825en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1642348690&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume100en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1411en_HK
dc.identifier.epage1417en_HK
dc.identifier.isiWOS:000220341000013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridKhoo, US=7004195799en_HK
dc.identifier.scopusauthoridLai, CYL=9276337700en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridXue, WC=7103165268en_HK
dc.identifier.scopusauthoridCheng, DKL=7402806161en_HK
dc.identifier.scopusauthoridChiu, PM=7103182596en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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