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Article: HPV and p53 in cervical cancer

TitleHPV and p53 in cervical cancer
Authors
Issue Date1994
PublisherB M J Publishing Group. The Journal's web site is located at http://sti.bmjjournals.com/
Citation
Genitourinary Medicine, 1994, v. 70 n. 3, p. 167-170 How to Cite?
AbstractObjective - To determine the prevalence of HPV 16 and 18 E6 by DNA detection and p53 abnormal protein expression in cervical cancers in Hong Kong. Materials and methods - Seventy-three squamous cell cervical cancer biopsy were analysed. Detection of HPV DNA was carried out by the polymerase chain reaction and Southern blotting (PCR/SB) technique using primers to the HPV16 and 18 E6 region and consensus primers to the L1 region. Abnormal expression of the p53 protein was detected by immunohistochemical staining (IHS) using the antibody CM1 on frozen sections of 55 cervical cancer samples. Forty-six samples were analysed for both the presence of HPV DNA and abnormal expression of p53. Results - 67.2% of the 64 samples showed the presence of HPV 16 E6 DNA and 39.1% showed the presence of HPV 18 E6 DNA. 32.8% showed the presence of both HPV 16 and 18 E6 DNA. No HPV DNA was shown in 10.9% of samples. Only 3.6% (2) of 55 samples showed positive IHS with CM1. One occurred in a HPV negative sample and the other in a HPV positive sample. Conclusion - A high prevalence of HPV DNA was detected in cervical cancer in Hong Kong using the PCR/SB technique. However, abnormal expression of p53 was uncommon amongst patients with or without HPV 16 or 18 infection.
Persistent Identifierhttp://hdl.handle.net/10722/45489
ISSN
1999 Impact Factor: 1.776
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorStanley, Men_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorMa, HKen_HK
dc.date.accessioned2007-10-30T06:27:13Z-
dc.date.available2007-10-30T06:27:13Z-
dc.date.issued1994en_HK
dc.identifier.citationGenitourinary Medicine, 1994, v. 70 n. 3, p. 167-170en_HK
dc.identifier.issn0266-4348en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45489-
dc.description.abstractObjective - To determine the prevalence of HPV 16 and 18 E6 by DNA detection and p53 abnormal protein expression in cervical cancers in Hong Kong. Materials and methods - Seventy-three squamous cell cervical cancer biopsy were analysed. Detection of HPV DNA was carried out by the polymerase chain reaction and Southern blotting (PCR/SB) technique using primers to the HPV16 and 18 E6 region and consensus primers to the L1 region. Abnormal expression of the p53 protein was detected by immunohistochemical staining (IHS) using the antibody CM1 on frozen sections of 55 cervical cancer samples. Forty-six samples were analysed for both the presence of HPV DNA and abnormal expression of p53. Results - 67.2% of the 64 samples showed the presence of HPV 16 E6 DNA and 39.1% showed the presence of HPV 18 E6 DNA. 32.8% showed the presence of both HPV 16 and 18 E6 DNA. No HPV DNA was shown in 10.9% of samples. Only 3.6% (2) of 55 samples showed positive IHS with CM1. One occurred in a HPV negative sample and the other in a HPV positive sample. Conclusion - A high prevalence of HPV DNA was detected in cervical cancer in Hong Kong using the PCR/SB technique. However, abnormal expression of p53 was uncommon amongst patients with or without HPV 16 or 18 infection.en_HK
dc.format.extent1030912 bytes-
dc.format.extent3017 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://sti.bmjjournals.com/en_HK
dc.relation.ispartofGenitourinary Medicineen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsGenitourinary Medicine. Copyright © B M J Publishing Group.en_HK
dc.subject.meshCarcinoma,-Squamous-Cell-microbiologyen_HK
dc.subject.meshCervix-Uteri-microbiologyen_HK
dc.subject.meshPapillomaviridae-isolation-and-purificationen_HK
dc.subject.meshTumor-Suppressor-Protein-p53-analysisen_HK
dc.subject.meshUterine-Cervical-Neoplasms-microbiologyen_HK
dc.titleHPV and p53 in cervical canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0266-4348&volume=70&issue=3&spage=167&epage=170&date=1994&atitle=HPV+and+p53+in+cervical+canceren_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.emailLiu, SS:stephasl@hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8039779-
dc.identifier.pmcidPMC1195224-
dc.identifier.scopuseid_2-s2.0-0028364998en_HK
dc.identifier.volume70en_HK
dc.identifier.issue3en_HK
dc.identifier.spage167en_HK
dc.identifier.epage170en_HK
dc.identifier.isiWOS:A1994NR80000003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridStanley, M=7202497085en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridMa, HK=7403095603en_HK

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