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Conference Paper: Annexin II-binding immunoglobulin level correlates with renal histological features in lupus nephritis

TitleAnnexin II-binding immunoglobulin level correlates with renal histological features in lupus nephritis
Authors
Issue Date2016
PublisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org
Citation
The 2016 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2016), Chicago, IL., 15-20 November 2016. In Journal of the American Society of Nephrology, 2016, v. 27 abstract suppl., p. 392A, abstract FR-PO119 How to Cite?
AbstractBACKGROUND: Annexin II on the surface of mesangial cells mediates anti-dsDNA antibody binding and downstream inflammatory and fibrotic processes in lupus nephritis. We investigated the relationship between annexin II-binding immunoglobulins and renal histology in lupus nephritis. METHODS: Archived serial serum samples from 28 patients with Class III/IV lupus nephritis were retrieved, and annexin II-binding immunoglobulin level was measured with an in-house ELISA. Glomeruli were isolated from NZBWF1 mice, and annexin II gene and protein expression was investigated by real-time PCR and cytochemical staining respectively. Ultrastructural localization of annexin II was determined by immunogold staining and electron microscopy. RESULTS: Associations between serum annexin II-binding IgG level, anti-dsDNA level, and disease activity were observed in 42% of lupus nephritis patients. Annexin II-binding IgG level correlated with activity index (r=0.44, p=0.04), leukocyte infiltration score (r=0.52, p=0.01), and karyorrhexis/fibrinoid necrosis score (r=0.66, p=0.002) as stated in the patients’ renal biopsy reports, and also the semi-quantitative mesangial electron-dense deposit score (r=0.63, p=0.009). Glomerular annexin II expression increased with active nephritis and decreased with glomerulosclerosis in NZBWF1 mice. Annexin II expression co-localized with electron-dense deposits in the mesangium, along the glomerular basement membrane and around podocytes. CONCLUSIONS: The association between annexin II-binding IgG level and histological features of severe nephritis and the co-localization of annexin II with electron-dense deposits both implicate annexin II in the pathogenesis of lupus nephritis. Funding: Government Support ‑ Non-U.S.
DescriptionPoster/Friday: Basic/Experimental Immunology - 1: no. FR-PO119
Persistent Identifierhttp://hdl.handle.net/10722/235033
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699

 

DC FieldValueLanguage
dc.contributor.authorCheung, KF-
dc.contributor.authorYung, SSY-
dc.contributor.authorChau, KM-
dc.contributor.authorYap, YHD-
dc.contributor.authorChan, DTM-
dc.date.accessioned2016-10-14T13:50:50Z-
dc.date.available2016-10-14T13:50:50Z-
dc.date.issued2016-
dc.identifier.citationThe 2016 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2016), Chicago, IL., 15-20 November 2016. In Journal of the American Society of Nephrology, 2016, v. 27 abstract suppl., p. 392A, abstract FR-PO119-
dc.identifier.issn1046-6673-
dc.identifier.urihttp://hdl.handle.net/10722/235033-
dc.descriptionPoster/Friday: Basic/Experimental Immunology - 1: no. FR-PO119-
dc.description.abstractBACKGROUND: Annexin II on the surface of mesangial cells mediates anti-dsDNA antibody binding and downstream inflammatory and fibrotic processes in lupus nephritis. We investigated the relationship between annexin II-binding immunoglobulins and renal histology in lupus nephritis. METHODS: Archived serial serum samples from 28 patients with Class III/IV lupus nephritis were retrieved, and annexin II-binding immunoglobulin level was measured with an in-house ELISA. Glomeruli were isolated from NZBWF1 mice, and annexin II gene and protein expression was investigated by real-time PCR and cytochemical staining respectively. Ultrastructural localization of annexin II was determined by immunogold staining and electron microscopy. RESULTS: Associations between serum annexin II-binding IgG level, anti-dsDNA level, and disease activity were observed in 42% of lupus nephritis patients. Annexin II-binding IgG level correlated with activity index (r=0.44, p=0.04), leukocyte infiltration score (r=0.52, p=0.01), and karyorrhexis/fibrinoid necrosis score (r=0.66, p=0.002) as stated in the patients’ renal biopsy reports, and also the semi-quantitative mesangial electron-dense deposit score (r=0.63, p=0.009). Glomerular annexin II expression increased with active nephritis and decreased with glomerulosclerosis in NZBWF1 mice. Annexin II expression co-localized with electron-dense deposits in the mesangium, along the glomerular basement membrane and around podocytes. CONCLUSIONS: The association between annexin II-binding IgG level and histological features of severe nephritis and the co-localization of annexin II with electron-dense deposits both implicate annexin II in the pathogenesis of lupus nephritis. Funding: Government Support ‑ Non-U.S.-
dc.languageeng-
dc.publisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org-
dc.relation.ispartofJournal of the American Society of Nephrology-
dc.titleAnnexin II-binding immunoglobulin level correlates with renal histological features in lupus nephritis-
dc.typeConference_Paper-
dc.identifier.emailCheung, KF: skfc819@hku.hk-
dc.identifier.emailYung, SSY: ssyyung@hku.hk-
dc.identifier.emailChau, KM: melchau@hkucc.hku.hk-
dc.identifier.emailYap, YHD: desmondy@hku.hk-
dc.identifier.emailChan, DTM: dtmchan@hkucc.hku.hk-
dc.identifier.authorityYung, SSY=rp00455-
dc.identifier.authorityYap, YHD=rp01607-
dc.identifier.authorityChan, DTM=rp00394-
dc.identifier.hkuros269038-
dc.identifier.volume27-
dc.identifier.issueabstract suppl.-
dc.identifier.spage392A, abstract FR-PO119-
dc.identifier.epage392A, abstract FR-PO119-
dc.publisher.placeUnited States-

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