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Conference Paper: Binding activity of serum immunoglobulin G to renal proximal tubular epithelial cells and its clinical correlation in lupus nephritis
Title | Binding activity of serum immunoglobulin G to renal proximal tubular epithelial cells and its clinical correlation in lupus nephritis |
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Authors | |
Issue Date | 2015 |
Publisher | Oxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/ |
Citation | The 52th European Renal Association/European Dialysis Transplantation Association (ERA-EDTA) Congress, London, UK., 28-31 May 2015. In Nephrology Dialysis Transplantation, 2015, v. 30 suppl. 3, no. FP149 How to Cite? |
Abstract | INTRODUCTION AND AIMS: In vitro data suggested that immunoglobulin G (IgG) from lupus nephritis (LN) patients could bind to proximal renal tubular epithelial cells (PTEC), but its clinical relevance remained to be defined. METHODS: The binding activities of IgG and its subclasses to PTEC were measured with cellular ELISA in 189 serial serum samples from 23 Class III/IV±V LN patients (48 during renal flares, 141 during remission), and compared with samples from 64 patients with non-lupus glomerular diseases (NLGD) and 23 healthy controls. The binding indices of serum IgG to PTEC were expressed as OD. RESULTS: Total IgG PTEC-binding indices were 0.34±0.16, 0.29±0.16, 0.62±0.27 and 0.83±0.38 in healthy controls, NLGD, LN patients during remission, and LN patients during renal flare (P<0.001, remission vs. renal flare; P<0.001, healthy controls or NLGD vs. LN during remission or renal flare). PTEC-binding index for IgG1 was 0.09 ±0.05, 0.16±0.12, 0.44±0.34 and 0.71±0.46 for the corresponding groups (P<0.001, remission vs. renal flare; P<0.001, healthy controls or NLGD vs. LN during remission or renal flare). No significant binding to PTEC was observed for IgG2, IgG3 or IgG4. Sixteen of 48 episodes (33.3%) of nephritic flares, despite clinical response to immunosuppressive therapies, showed persistent seropositivity (mean duration 9.4±3.1 months) for PTEC-binding IgG. Total IgG and IgG1 PTEC-binding demonstrated a positive association with anti-dsDNA titres (r=0.34 and 0.52 respectively, P<0.001 for both), but a negative correlation with C3 level (r=-0.26 and -0.50 respectively, P=0.002 and <0.001). Sensitivity/specificity of PTEC-binding index in predicting renal flares was 45.8%/80.1% for total IgG (ROC AUC 0.630, P=0.007) and 87.5%/35.5% for IgG1 (ROC AUC 0.615, P=0.018). IgG1 PTEC-binding index correlated with tubulo-interstitial inflammation score in renal biopsies from corresponding patients. CONCLUSIONS: Total IgG and IgG1 PTEC-binding index in LN patients correlated with clinical and serological activity, and in combination could predict LN flares. |
Description | Session - Clinical Nephrology, Primary and Secondary Glomerulonephritis - 1: no. FP149 This free journal suppl. entitled: 52nd ERA-EDTA Congress, May 28th-31st, 2015, London, United Kingdom |
Persistent Identifier | http://hdl.handle.net/10722/211455 |
ISSN | 2023 Impact Factor: 4.8 2023 SCImago Journal Rankings: 1.414 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Yap, DYH | - |
dc.contributor.author | Yung, S | - |
dc.contributor.author | Zhang, Q | - |
dc.contributor.author | Chan, TM | - |
dc.date.accessioned | 2015-07-14T06:42:23Z | - |
dc.date.available | 2015-07-14T06:42:23Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The 52th European Renal Association/European Dialysis Transplantation Association (ERA-EDTA) Congress, London, UK., 28-31 May 2015. In Nephrology Dialysis Transplantation, 2015, v. 30 suppl. 3, no. FP149 | - |
dc.identifier.issn | 0931-0509 | - |
dc.identifier.uri | http://hdl.handle.net/10722/211455 | - |
dc.description | Session - Clinical Nephrology, Primary and Secondary Glomerulonephritis - 1: no. FP149 | - |
dc.description | This free journal suppl. entitled: 52nd ERA-EDTA Congress, May 28th-31st, 2015, London, United Kingdom | - |
dc.description.abstract | INTRODUCTION AND AIMS: In vitro data suggested that immunoglobulin G (IgG) from lupus nephritis (LN) patients could bind to proximal renal tubular epithelial cells (PTEC), but its clinical relevance remained to be defined. METHODS: The binding activities of IgG and its subclasses to PTEC were measured with cellular ELISA in 189 serial serum samples from 23 Class III/IV±V LN patients (48 during renal flares, 141 during remission), and compared with samples from 64 patients with non-lupus glomerular diseases (NLGD) and 23 healthy controls. The binding indices of serum IgG to PTEC were expressed as OD. RESULTS: Total IgG PTEC-binding indices were 0.34±0.16, 0.29±0.16, 0.62±0.27 and 0.83±0.38 in healthy controls, NLGD, LN patients during remission, and LN patients during renal flare (P<0.001, remission vs. renal flare; P<0.001, healthy controls or NLGD vs. LN during remission or renal flare). PTEC-binding index for IgG1 was 0.09 ±0.05, 0.16±0.12, 0.44±0.34 and 0.71±0.46 for the corresponding groups (P<0.001, remission vs. renal flare; P<0.001, healthy controls or NLGD vs. LN during remission or renal flare). No significant binding to PTEC was observed for IgG2, IgG3 or IgG4. Sixteen of 48 episodes (33.3%) of nephritic flares, despite clinical response to immunosuppressive therapies, showed persistent seropositivity (mean duration 9.4±3.1 months) for PTEC-binding IgG. Total IgG and IgG1 PTEC-binding demonstrated a positive association with anti-dsDNA titres (r=0.34 and 0.52 respectively, P<0.001 for both), but a negative correlation with C3 level (r=-0.26 and -0.50 respectively, P=0.002 and <0.001). Sensitivity/specificity of PTEC-binding index in predicting renal flares was 45.8%/80.1% for total IgG (ROC AUC 0.630, P=0.007) and 87.5%/35.5% for IgG1 (ROC AUC 0.615, P=0.018). IgG1 PTEC-binding index correlated with tubulo-interstitial inflammation score in renal biopsies from corresponding patients. CONCLUSIONS: Total IgG and IgG1 PTEC-binding index in LN patients correlated with clinical and serological activity, and in combination could predict LN flares. | - |
dc.language | eng | - |
dc.publisher | Oxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/ | - |
dc.relation.ispartof | Nephrology Dialysis Transplantation | - |
dc.title | Binding activity of serum immunoglobulin G to renal proximal tubular epithelial cells and its clinical correlation in lupus nephritis | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yap, DYH: desmondy@hku.hk | - |
dc.identifier.email | Yung, S: ssyyung@hku.hk | - |
dc.identifier.email | Chan, TM: dtmchan@hkucc.hku.hk | - |
dc.identifier.authority | Yap, DYH=rp01607 | - |
dc.identifier.authority | Yung, S=rp00455 | - |
dc.identifier.authority | Chan, TM=rp00394 | - |
dc.identifier.doi | 10.1093/ndt/gfv171.38 | - |
dc.identifier.hkuros | 245024 | - |
dc.identifier.volume | 30 | - |
dc.identifier.issue | suppl. 3 | - |
dc.identifier.isi | WOS:000361215100198 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0931-0509 | - |