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  Patent History
  • Application
    PC T/CN2008001435 2008-08-08
  • Publication
    WO 2009024019 2009-02-26
Supplementary

published patent: METHODS AND COMPOSITIONS FOR HIGH-THROUGHPUT BISULPHITE DNA-SEQUENCING AND UTILITIES

TitleMETHODS AND COMPOSITIONS FOR HIGH-THROUGHPUT BISULPHITE DNA-SEQUENCING AND UTILITIES
Priority Date2007-08-15 US 11/935472P
2007-09-05 US 11/935867P
Inventors
Issue Date2009
Citation
WO Published patent application WO 2009024019. World Intellectual Property Organization (WIPO), PatentScope, 2009 How to Cite?
AbstractThe novel methods and compositions to produce DNA templates suitable for chemical modifications and high-throughput DNA-sequencing are disclosed. A method of a DNA adaptor design where constituent cytosines are substituted with 5-methylcytosines rendering the resulting adaptor resistant to bisulphite mediated deamination is also disclosed. When said adaptor is ligated onto double stranded DNA template, subsequent DNA denaturation and bisulphite treatment deaminates template DNA cytosine differentially to uracil whilst the 5-methylcytosines of the ligated adaptor resist chemical conversion resulting in the adaptor sequence remaining unaltered. Both strands of bisulphite treated DNA can thus be amplified with a single primer set that hybridizes to the unaltered adaptor sequence. Also the methods to produce control template of a defined methylation composition to optimize conditions for the bisulphite reaction are disclosed. In a preferred embodiment, the present invent can be used to produce templates suitable for genome-wide bisulphite-DNA sequencing using conventional, SolexaTM, SOLiDTM or 454TM_ type DNA sequencing platforms to study DNA methylation.
Persistent Identifierhttp://hdl.handle.net/10722/177133
References

 

DC FieldValueLanguage
dc.date.accessioned2012-11-30T08:39:11Z-
dc.date.available2012-11-30T08:39:11Z-
dc.date.issued2009-
dc.identifier.citationWO Published patent application WO 2009024019. World Intellectual Property Organization (WIPO), PatentScope, 2009en_HK
dc.identifier.urihttp://hdl.handle.net/10722/177133-
dc.description.abstractThe novel methods and compositions to produce DNA templates suitable for chemical modifications and high-throughput DNA-sequencing are disclosed. A method of a DNA adaptor design where constituent cytosines are substituted with 5-methylcytosines rendering the resulting adaptor resistant to bisulphite mediated deamination is also disclosed. When said adaptor is ligated onto double stranded DNA template, subsequent DNA denaturation and bisulphite treatment deaminates template DNA cytosine differentially to uracil whilst the 5-methylcytosines of the ligated adaptor resist chemical conversion resulting in the adaptor sequence remaining unaltered. Both strands of bisulphite treated DNA can thus be amplified with a single primer set that hybridizes to the unaltered adaptor sequence. Also the methods to produce control template of a defined methylation composition to optimize conditions for the bisulphite reaction are disclosed. In a preferred embodiment, the present invent can be used to produce templates suitable for genome-wide bisulphite-DNA sequencing using conventional, SolexaTM, SOLiDTM or 454TM_ type DNA sequencing platforms to study DNA methylation.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License For Public Patent Documentsen_US
dc.titleMETHODS AND COMPOSITIONS FOR HIGH-THROUGHPUT BISULPHITE DNA-SEQUENCING AND UTILITIESen_HK
dc.typePatenten_US
dc.description.naturepublished_or_final_versionen_US
dc.contributor.inventorLok, Sen_HK
patents.identifier.applicationPC T/CN2008001435en_HK
patents.description.assigneeUNIV HONG KONG [CN]; LOK SI [CN]en_HK
patents.description.countryWorld Intellectual Property Organization (WIPO)en_HK
patents.date.publication2009-02-26en_HK
dc.relation.referencesWO 2005090607 (A1) 2005-09-29en_HK
dc.relation.referencesWO 2008096146 (A1) 2008-08-14en_HK
dc.relation.referencesEP 1568786 (A2) 2005-08-31en_HK
dc.relation.referencesEP 1568786 (A3) 2007-08-29en_HK
dc.relation.referencesUS 2005009059 (A1) 2005-01-13en_HK
dc.relation.referencesUS 2005153347 (A1) 2005-07-14en_HK
dc.relation.referencesCN 1415761 (A) 2003-05-07en_HK
patents.date.application2008-08-08en_HK
patents.date.priority2007-08-15 US 11/935472Pen_HK
patents.date.priority2007-09-05 US 11/935867Pen_HK
patents.description.ccWOen_HK
patents.identifier.publicationWO 2009024019en_HK
patents.relation.familyCN 101802223 (A) 2010-08-11en_HK
patents.relation.familyEP 2188389 (A1) 2010-05-26en_HK
patents.relation.familyJP 2010535513 (A) 2010-11-25en_HK
patents.relation.familyUS 2009047680 (A1) 2009-02-19en_HK
patents.relation.familyWO 2009024019 (A1) 2009-02-26en_HK
patents.description.kindA1en_HK
patents.typePatent_publisheden_HK

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