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  Patent History
  • Application
    CN 20088103541 2008-08-08
  • Publication
    CN 101802223 2010-08-11
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published patent: Methods and compositions for high-throughput bisulphite DNA-sequencing and utilities

TitleMethods and compositions for high-throughput bisulphite DNA-sequencing and utilities
Priority Date2008-08-08 PCT/CN2008001435
2007-08-15 US 11/935472P
2007-09-05 US 11/935867P
Inventors
Issue Date2010
Citation
China Published Patent Application CN 101802223. Beijing, PRC: State Intellectual Property Office (SIPO) of the P.R.C., 2010 How to Cite?
AbstractThe novel methods and compositions to produce DNA templates suitable for chemical modifications and high-throughput DNA-sequencing are disclosed. A method of a DNA adaptor design where constituent cytosines are substituted with 5-methylcytosines rendering the resulting adaptor resistant to bisulphite mediated deamination is also disclosed. When said adaptor is ligated onto double stranded DNA template, subsequent DNA denaturation and bisulphite treatment deaminates template DNA cytosine differentially to uracil whilst the 5-methylcytosines of the ligated adaptor resist chemical conversion resulting in the adaptor sequence remaining unaltered. Both strands of bisulphite treated DNA can thus be amplified with a single primer set that hybridizes to the unaltered adaptor sequence. Also the methods to produce control template of a defined methylation composition to optimize conditions for the bisulphite reaction are disclosed. In a preferred embodiment, the present invent can be used to produce templates suitable for genome-wide bisulphite-DNA sequencing using conventional, SolexaTM, SOLiDTM or 454TM_ type DNA sequencing platforms to study DNA methylation.
Persistent Identifierhttp://hdl.handle.net/10722/176630

 

DC FieldValueLanguage
dc.date.accessioned2012-11-30T08:38:16Z-
dc.date.available2012-11-30T08:38:16Z-
dc.date.issued2010-
dc.identifier.citationChina Published Patent Application CN 101802223. Beijing, PRC: State Intellectual Property Office (SIPO) of the P.R.C., 2010en_HK
dc.identifier.urihttp://hdl.handle.net/10722/176630-
dc.description.abstractThe novel methods and compositions to produce DNA templates suitable for chemical modifications and high-throughput DNA-sequencing are disclosed. A method of a DNA adaptor design where constituent cytosines are substituted with 5-methylcytosines rendering the resulting adaptor resistant to bisulphite mediated deamination is also disclosed. When said adaptor is ligated onto double stranded DNA template, subsequent DNA denaturation and bisulphite treatment deaminates template DNA cytosine differentially to uracil whilst the 5-methylcytosines of the ligated adaptor resist chemical conversion resulting in the adaptor sequence remaining unaltered. Both strands of bisulphite treated DNA can thus be amplified with a single primer set that hybridizes to the unaltered adaptor sequence. Also the methods to produce control template of a defined methylation composition to optimize conditions for the bisulphite reaction are disclosed. In a preferred embodiment, the present invent can be used to produce templates suitable for genome-wide bisulphite-DNA sequencing using conventional, SolexaTM, SOLiDTM or 454TM_ type DNA sequencing platforms to study DNA methylation.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License For Public Patent Documentsen_US
dc.titleMethods and compositions for high-throughput bisulphite DNA-sequencing and utilitiesen_HK
dc.typePatenten_US
dc.description.naturepublished_or_final_versionen_US
dc.contributor.inventorLok, Sen_HK
patents.identifier.applicationCN 20088103541en_HK
patents.description.assigneeUNIV HONG KONGen_HK
patents.description.countryChina, PRCen_HK
patents.date.publication2010-08-11en_HK
patents.date.application2008-08-08en_HK
patents.date.priority2008-08-08 PCT/CN2008001435en_HK
patents.date.priority2007-08-15 US 11/935472Pen_HK
patents.date.priority2007-09-05 US 11/935867Pen_HK
patents.description.ccCNen_HK
patents.identifier.publicationCN 101802223en_HK
patents.relation.familyCN 101802223 (A) 2010-08-11en_HK
patents.relation.familyEP 2188389 (A1) 2010-05-26en_HK
patents.relation.familyJP 2010535513 (A) 2010-11-25en_HK
patents.relation.familyUS 2009047680 (A1) 2009-02-19en_HK
patents.relation.familyWO 2009024019 (A1) 2009-02-26en_HK
patents.description.kindAen_HK
patents.typePatent_publisheden_HK

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