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Article: Overestimation of the prevalence of antibody to hepatitis C virus in retrospective studies on stored sera

TitleOverestimation of the prevalence of antibody to hepatitis C virus in retrospective studies on stored sera
Authors
Issue Date1991
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
Hepatology, 1991, v. 14 n. 5, p. 756-762 How to Cite?
AbstractSerum samples (1,428) from 1,149 patients with chronic liver diseases and polytransfused subjects were tested for antibody to hepatitis C virus by first-generation enzyme immunoassays. Antibody to hepatitis C virus was detected in 87% of patients with transfusion-related chronic non-A, non-B hepatitis; 17.6% of patients with nonmalignant, chronic hepatitis B virus infection; 37.3% of patients with hepatocellular carcinoma; 14.3% of patients with alcoholic liver diseases; 22.2% of patients with cryptogenic cirrhosis; 76% of intravenous drug abusers; 16.4% of patients on hemodialysis; 1.8% of patients on peritoneal dialysis; 6.2% of kidney transplant recipients; and 3.1% of normal subjects. A high frequency of weakly positive results was found in ''aged'' samples: 61.9% of antibody to hepatitis C virus-positive patients whose sera had been stored for more than 2 yr had optical densities less than two times the cut-off values in contrast to 7.9% of those whose sera had been stored for less than 2 yr (p < 0.0001). A significantly lower proportion of patients who had optical densities less than two times the cut-off values were reactive in subsequent samples, 27.5% vs. 87.5% (p < 0.0001). On retests, only 70% and 56% of sera that were initially positive for antibody to hepatitis C virus remained antibody to hepatitis C virus positive using second-generation enzyme immunoassays and neutralization enzyme immunoassays, respectively. Our results suggest that retrospective studies on stored sera may have overestimated the prevalence of antibody to hepatitis C virus. We recommend that care be exercised in the interpretation of the results of the first-generation enzyme immunoassays, especially with weakly positive results on ''aged'' sera.
Persistent Identifierhttp://hdl.handle.net/10722/161878
ISSN
2015 Impact Factor: 11.711
2015 SCImago Journal Rankings: 4.752

 

DC FieldValueLanguage
dc.contributor.authorLok, ASFen_US
dc.contributor.authorMa, OCKen_US
dc.contributor.authorChan, TMen_US
dc.contributor.authorLai, CLen_US
dc.contributor.authorChung, HTen_US
dc.contributor.authorNg, CPLen_US
dc.contributor.authorLam, JSCen_US
dc.date.accessioned2012-09-05T05:15:43Z-
dc.date.available2012-09-05T05:15:43Z-
dc.date.issued1991en_US
dc.identifier.citationHepatology, 1991, v. 14 n. 5, p. 756-762en_US
dc.identifier.issn0270-9139en_US
dc.identifier.urihttp://hdl.handle.net/10722/161878-
dc.description.abstractSerum samples (1,428) from 1,149 patients with chronic liver diseases and polytransfused subjects were tested for antibody to hepatitis C virus by first-generation enzyme immunoassays. Antibody to hepatitis C virus was detected in 87% of patients with transfusion-related chronic non-A, non-B hepatitis; 17.6% of patients with nonmalignant, chronic hepatitis B virus infection; 37.3% of patients with hepatocellular carcinoma; 14.3% of patients with alcoholic liver diseases; 22.2% of patients with cryptogenic cirrhosis; 76% of intravenous drug abusers; 16.4% of patients on hemodialysis; 1.8% of patients on peritoneal dialysis; 6.2% of kidney transplant recipients; and 3.1% of normal subjects. A high frequency of weakly positive results was found in ''aged'' samples: 61.9% of antibody to hepatitis C virus-positive patients whose sera had been stored for more than 2 yr had optical densities less than two times the cut-off values in contrast to 7.9% of those whose sera had been stored for less than 2 yr (p < 0.0001). A significantly lower proportion of patients who had optical densities less than two times the cut-off values were reactive in subsequent samples, 27.5% vs. 87.5% (p < 0.0001). On retests, only 70% and 56% of sera that were initially positive for antibody to hepatitis C virus remained antibody to hepatitis C virus positive using second-generation enzyme immunoassays and neutralization enzyme immunoassays, respectively. Our results suggest that retrospective studies on stored sera may have overestimated the prevalence of antibody to hepatitis C virus. We recommend that care be exercised in the interpretation of the results of the first-generation enzyme immunoassays, especially with weakly positive results on ''aged'' sera.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/en_US
dc.relation.ispartofHepatologyen_US
dc.subject.meshAntibodies, Viral - Analysisen_US
dc.subject.meshBlood - Immunologyen_US
dc.subject.meshBlood Preservationen_US
dc.subject.meshBlood Transfusionen_US
dc.subject.meshChronic Diseaseen_US
dc.subject.meshHepacivirus - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshLiver Diseases - Immunologyen_US
dc.subject.meshRetrospective Studiesen_US
dc.subject.meshTime Factorsen_US
dc.titleOverestimation of the prevalence of antibody to hepatitis C virus in retrospective studies on stored seraen_US
dc.typeArticleen_US
dc.identifier.emailChan, TM:dtmchan@hku.hken_US
dc.identifier.emailLai, CL:hrmelcl@hku.hken_US
dc.identifier.authorityChan, TM=rp00394en_US
dc.identifier.authorityLai, CL=rp00314en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/hep.1840140503en_US
dc.identifier.pmid1657751-
dc.identifier.scopuseid_2-s2.0-0025889073en_US
dc.identifier.volume14en_US
dc.identifier.issue5en_US
dc.identifier.spage756en_US
dc.identifier.epage762en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLok, ASF=35379868500en_US
dc.identifier.scopusauthoridMa, OCK=7004452841en_US
dc.identifier.scopusauthoridChan, TM=7402687700en_US
dc.identifier.scopusauthoridLai, CL=7403086396en_US
dc.identifier.scopusauthoridChung, HT=7404007053en_US
dc.identifier.scopusauthoridNg, CPL=7401705360en_US
dc.identifier.scopusauthoridLam, JSC=16151639300en_US

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