File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Binding of serum IgG to human mesangial cells and its correlation with disease activity in patients with lupus nephritis

TitleBinding of serum IgG to human mesangial cells and its correlation with disease activity in patients with lupus nephritis
Authors
Issue Date2011
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2326-5205
Citation
The 75th ACR/ARHP Annual Scientific Meeting, Chicago, IL., 4-9 November 2011. In Arthritis & Rheumatism, 2011, v. 63 n. 10 suppl., p. S561, abstract no. 1435 How to Cite?
AbstractBACKGROUND/PURPOSE: Lupus nephritis is hallmarked by mesangial deposition of immunoglobulins, which result in subsequent glomerular injury and altered renal functions. Our group had previously demonstrated that human anti-dsDNA antibodies could bind to human mesangial cells (HMC) and this binding activity correlated with disease activity. In this study we assessed the binding activity to HMC by the serum IgG and its subclasses in lupus nephritis patients. Their associations with clinical and laboratory parameters in lupus nephritis patients were also evaluated. METHODS: Serial serum samples were retrieved from 23 patients with biopsy-proven diffuse proliferative lupus nephritis over a mean follow-up of 74 months. Binding activity (expressed as OD) of total serum IgG and its subclasses (IgG1, IgG2, IgG3, IgG4) to HMC was ascertained with a cellular ELISA and its correlation with clinical or laboratory parameters examined. Sera from 23 healthy individuals were used as controls. Sensitivity/specificity of total IgG or IgG1 binding to HMC in the prediction of renal flare was calculated and ROC curves constructed. RESULTS: A total of 189 samples were analyzed - 48 samples during active and 141 during inactive disease, defined according to clinical assessment (SLEDAI > =10 as active and < = 4 as inactive). Binding of serum total IgG to HMC was 0.12±0.09, 0.59±0.37 and 0.74±0.42 OD for healthy controls, inactive lupus, and active lupus respectively (P = 0.023 active vs inactive, P < 0.001 controls vs active or inactive disease). Binding of serum IgG1 to HMC was 0.05±0.05, 0.41±0.38 and 0.55±0.40 OD for the three groups respectively (P = 0.037 active vs inactive, P < 0.001 controls vs active or inactive disease). Controls and lupus patients did not vary in the binding of serum IgG2, IgG3 or IgG4 to HMC. Total IgG and IgG1 HMC-binding activity correlated with anti-dsDNA levels (r_0.26 and 0.39 respectively, P < 0.001 for both), and inversely with C3 levels (r = -0.17 and -0.45 respectively, P < 0.05 for both). No correlation was observed between IgG binding to HMC and clinical parameters such as serum creatinine, albumin, or proteinuria. Sensitivity/specificity of total IgG or IgG1 binding to HMC in the prediction of renal flare was 81.3%/39.7% (ROC AUC 0.61, P = 0.03) and 83.8%/41.8% (AUC 0.63, P = 0.009) respectively. CONCLUSION: There is significant total IgG and IgG1 mesangial cell-binding activity in the sera of patients with lupus nephritis, especially during active disease, and this binding correlated with the anti-dsDNA antibodies levels.
DescriptionACR/ARHP Poster Session B: Systemic Lupus Erythematosus - Human Etiology and Pathogenesis 2: Innate Immune System and Organ Damage: no. 1435
Persistent Identifierhttp://hdl.handle.net/10722/160351
ISSN
2015 SCImago Journal Rankings: 1.984

 

DC FieldValueLanguage
dc.contributor.authorYap, DYHen_US
dc.contributor.authorYung, Sen_US
dc.contributor.authorChan, Oen_US
dc.contributor.authorZhang, FQen_US
dc.contributor.authorChan, TMen_US
dc.date.accessioned2012-08-16T06:08:10Z-
dc.date.available2012-08-16T06:08:10Z-
dc.date.issued2011en_US
dc.identifier.citationThe 75th ACR/ARHP Annual Scientific Meeting, Chicago, IL., 4-9 November 2011. In Arthritis & Rheumatism, 2011, v. 63 n. 10 suppl., p. S561, abstract no. 1435en_US
dc.identifier.issn2326-5205-
dc.identifier.urihttp://hdl.handle.net/10722/160351-
dc.descriptionACR/ARHP Poster Session B: Systemic Lupus Erythematosus - Human Etiology and Pathogenesis 2: Innate Immune System and Organ Damage: no. 1435-
dc.description.abstractBACKGROUND/PURPOSE: Lupus nephritis is hallmarked by mesangial deposition of immunoglobulins, which result in subsequent glomerular injury and altered renal functions. Our group had previously demonstrated that human anti-dsDNA antibodies could bind to human mesangial cells (HMC) and this binding activity correlated with disease activity. In this study we assessed the binding activity to HMC by the serum IgG and its subclasses in lupus nephritis patients. Their associations with clinical and laboratory parameters in lupus nephritis patients were also evaluated. METHODS: Serial serum samples were retrieved from 23 patients with biopsy-proven diffuse proliferative lupus nephritis over a mean follow-up of 74 months. Binding activity (expressed as OD) of total serum IgG and its subclasses (IgG1, IgG2, IgG3, IgG4) to HMC was ascertained with a cellular ELISA and its correlation with clinical or laboratory parameters examined. Sera from 23 healthy individuals were used as controls. Sensitivity/specificity of total IgG or IgG1 binding to HMC in the prediction of renal flare was calculated and ROC curves constructed. RESULTS: A total of 189 samples were analyzed - 48 samples during active and 141 during inactive disease, defined according to clinical assessment (SLEDAI > =10 as active and < = 4 as inactive). Binding of serum total IgG to HMC was 0.12±0.09, 0.59±0.37 and 0.74±0.42 OD for healthy controls, inactive lupus, and active lupus respectively (P = 0.023 active vs inactive, P < 0.001 controls vs active or inactive disease). Binding of serum IgG1 to HMC was 0.05±0.05, 0.41±0.38 and 0.55±0.40 OD for the three groups respectively (P = 0.037 active vs inactive, P < 0.001 controls vs active or inactive disease). Controls and lupus patients did not vary in the binding of serum IgG2, IgG3 or IgG4 to HMC. Total IgG and IgG1 HMC-binding activity correlated with anti-dsDNA levels (r_0.26 and 0.39 respectively, P < 0.001 for both), and inversely with C3 levels (r = -0.17 and -0.45 respectively, P < 0.05 for both). No correlation was observed between IgG binding to HMC and clinical parameters such as serum creatinine, albumin, or proteinuria. Sensitivity/specificity of total IgG or IgG1 binding to HMC in the prediction of renal flare was 81.3%/39.7% (ROC AUC 0.61, P = 0.03) and 83.8%/41.8% (AUC 0.63, P = 0.009) respectively. CONCLUSION: There is significant total IgG and IgG1 mesangial cell-binding activity in the sera of patients with lupus nephritis, especially during active disease, and this binding correlated with the anti-dsDNA antibodies levels.-
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2326-5205-
dc.relation.ispartofArthritis & Rheumatismen_US
dc.rightsArthritis & Rheumatism. © John Wiley & Sons, Inc.-
dc.titleBinding of serum IgG to human mesangial cells and its correlation with disease activity in patients with lupus nephritisen_US
dc.typeConference_Paperen_US
dc.identifier.emailYap, DYH: desmondy@hku.hken_US
dc.identifier.emailYung, S: ssyyung@hku.hken_US
dc.identifier.emailChan, O: owenchan@hku.hken_US
dc.identifier.emailZhang, FQ: zhjhr@hkucc.hku.hken_US
dc.identifier.emailChan, TM: dtmchan@hku.hken_US
dc.identifier.authorityYap, DYH=rp01607en_US
dc.identifier.authorityYung, S=rp00455en_US
dc.identifier.authorityChan, TM=rp00394en_US
dc.identifier.hkuros205079en_US
dc.identifier.volume63-
dc.identifier.issue10 suppl.-
dc.identifier.spageS561, abstract no. 1435-
dc.identifier.epageS561, abstract no. 1435-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats