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Conference Paper: Serum IgG binding to human mesangial cells in patients with lupus nephritis and its correlation with disease activity

TitleSerum IgG binding to human mesangial cells in patients with lupus nephritis and its correlation with disease activity
Authors
Issue Date2011
PublisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org
Citation
The 45th Annual Meeting of the American Society of Nephrology (Kidney Week 2012), San Diego, CA., 30 October-4 November 4 2012. In Journal of the American Society of Nephrology, 2012, v. 23 abstract suppl., p. 623A, SA-PO2191 How to Cite?
AbstractBACKGROUND: Mesangial immunoglobulin deposition is a hallmark in lupus nephritis. We previously demonstrated that human anti-dsDNA antibodies could bind to human mesangial cells (HMC). In this study we investigated the binding activity of serum IgG to HMC and its correlation with clinical parameters in lupus patients. METHODS: Serial serum samples were obtained from 23 patients with biopsy-proven diffuse proliferative lupus nephritis over a mean follow-up of 74 months. Binding activity (expressed as OD) of total serum IgG and its subclasses (IgG1, IgG2, IgG3, IgG4) to HMC was measured using a cellular ELISA and its correlation with clinical or laboratory parameters investigated. Sera from 23 healthy individuals were used as controls. RESULTS: A total of 189 samples were collected - 48 samples during active and 141 during inactive disease, defined according to clinical assessment. Binding of serum total IgG to HMC was 0.12±0.09, 0.59±0.37 and 0.74±0.43 OD for healthy controls, inactive lupus, and active lupus respectively (P=0.023 active vs inactive, P<0.001 controls vs active or inactive disease). Binding of serum IgG1 to HMC was 0.05±0.05, 0.41±0.38 and 0.55±0.40 OD for the three groups respectively (P=0.037 active vs inactive, P<0.001 controls vs active or inactive disease). Controls and lupus patients did not differ in the binding of serum IgG2, IgG3 or IgG4 to HMC. HMC-binding activity of total IgG and IgG1 correlated with anti-dsDNA antibody levels (r=0.26 and 0.39 respectively, P<0.001 for both), and inversely correlated with C3 levels (r=-0.17 and -0.45 respectively, P<0.05 for both). No correlation was observed between IgG binding to HMC and clinical parameters such as serum creatinine, albumin, or proteinuria. Sensitivity/specificity of total IgG or IgG1 binding to HMC in the prediction of renal flare in the patients was 81.3%/39.7% (ROC AUC 0.61, P=0.03) and 83.3%/41.8% (AUC 0.63, P=0.009) respectively. CONCLUSIONS: We conclude that total IgG and IgG1 in the serum of patients with lupus nephritis bind significantly to mesangial cells, especially during flare, and this binding correlated with the level of anti-dsDNA antibodies.
DescriptionBasic/Experimental Immunology 2 (Poster): SA-PO2191
Persistent Identifierhttp://hdl.handle.net/10722/160347
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699

 

DC FieldValueLanguage
dc.contributor.authorYap, DYHen_US
dc.contributor.authorYung, Sen_US
dc.contributor.authorChan, Oen_US
dc.contributor.authorZhang, Qen_US
dc.contributor.authorChan, DTMen_US
dc.date.accessioned2012-08-16T06:08:09Z-
dc.date.available2012-08-16T06:08:09Z-
dc.date.issued2011en_US
dc.identifier.citationThe 45th Annual Meeting of the American Society of Nephrology (Kidney Week 2012), San Diego, CA., 30 October-4 November 4 2012. In Journal of the American Society of Nephrology, 2012, v. 23 abstract suppl., p. 623A, SA-PO2191en_US
dc.identifier.issn1046-6673-
dc.identifier.urihttp://hdl.handle.net/10722/160347-
dc.descriptionBasic/Experimental Immunology 2 (Poster): SA-PO2191-
dc.description.abstractBACKGROUND: Mesangial immunoglobulin deposition is a hallmark in lupus nephritis. We previously demonstrated that human anti-dsDNA antibodies could bind to human mesangial cells (HMC). In this study we investigated the binding activity of serum IgG to HMC and its correlation with clinical parameters in lupus patients. METHODS: Serial serum samples were obtained from 23 patients with biopsy-proven diffuse proliferative lupus nephritis over a mean follow-up of 74 months. Binding activity (expressed as OD) of total serum IgG and its subclasses (IgG1, IgG2, IgG3, IgG4) to HMC was measured using a cellular ELISA and its correlation with clinical or laboratory parameters investigated. Sera from 23 healthy individuals were used as controls. RESULTS: A total of 189 samples were collected - 48 samples during active and 141 during inactive disease, defined according to clinical assessment. Binding of serum total IgG to HMC was 0.12±0.09, 0.59±0.37 and 0.74±0.43 OD for healthy controls, inactive lupus, and active lupus respectively (P=0.023 active vs inactive, P<0.001 controls vs active or inactive disease). Binding of serum IgG1 to HMC was 0.05±0.05, 0.41±0.38 and 0.55±0.40 OD for the three groups respectively (P=0.037 active vs inactive, P<0.001 controls vs active or inactive disease). Controls and lupus patients did not differ in the binding of serum IgG2, IgG3 or IgG4 to HMC. HMC-binding activity of total IgG and IgG1 correlated with anti-dsDNA antibody levels (r=0.26 and 0.39 respectively, P<0.001 for both), and inversely correlated with C3 levels (r=-0.17 and -0.45 respectively, P<0.05 for both). No correlation was observed between IgG binding to HMC and clinical parameters such as serum creatinine, albumin, or proteinuria. Sensitivity/specificity of total IgG or IgG1 binding to HMC in the prediction of renal flare in the patients was 81.3%/39.7% (ROC AUC 0.61, P=0.03) and 83.3%/41.8% (AUC 0.63, P=0.009) respectively. CONCLUSIONS: We conclude that total IgG and IgG1 in the serum of patients with lupus nephritis bind significantly to mesangial cells, especially during flare, and this binding correlated with the level of anti-dsDNA antibodies.-
dc.languageengen_US
dc.publisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org-
dc.relation.ispartofJournal of the American Society of Nephrologyen_US
dc.relation.ispartofKidney Week 2012-
dc.titleSerum IgG binding to human mesangial cells in patients with lupus nephritis and its correlation with disease activityen_US
dc.typeConference_Paperen_US
dc.identifier.emailYap, DYH: desmondy@hku.hken_US
dc.identifier.emailYung, S: ssyyung@hku.hken_US
dc.identifier.emailChan, O: owenchan@hku.hken_US
dc.identifier.emailZhang, Q: zhjhr@hkucc.hku.hken_US
dc.identifier.emailChan, DTM: dtmchan@hku.hken_US
dc.identifier.authorityYap, DYH=rp01607en_US
dc.identifier.authorityYung, S=rp00455en_US
dc.identifier.authorityChan, DTM=rp00394en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros205064en_US
dc.identifier.volume2011-
dc.identifier.volume23-
dc.identifier.issuemeeting abstracts-
dc.identifier.spage623A, SA-PO2191-
dc.identifier.epage623A, SA-PO2191-
dc.publisher.placeUnited States-

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